1. A Guide to Microscopy

2. The Light Microscope Purpose: History Flea glasses Anton van Leeuwenhoek Robert Hooke

3. Anton “The Great” Van Leeuwenhoek Father of microscopy Apprenticeship Created the first light microscope

4. Robert Hooke First to see cells Observed cells in cork

5. The Compound Light Microscope Uses 2 or more lenses to view a sample Objective lens Ocular lens Light Source Advantages and Limitations

7. Parts of Interest See homework for functions Diaphragm Revolving nose piece Course adjustment knob Fine adjustment knob Which knob to use during high power?

8. Magnificaiton Definition: Eye Piece: _____ Scanning Power: ____ Low Power: ____ High Power: ____ How do you calculate total magnification?

9. Resolution Definition: Bad MicroscopeGood Microscope

10. Limitations of Light Microscope Poor Magnification Poor Resolution WHY???

11. Stereomicroscope Dissecting microscope: Why? Reflects light off of the surface Results in a ___ Image

12. Electron Microscopes 2 types: Transmission Electron Microscope (TEM) Scanning Electron Microscope (SEM) Electrons either pass through a specimen or bounce off of the surface to provide an image Resolution and Magnification?

14. Images obtained from EM’s

15. Electron Microscopes Advantages? Limitations?