1. 1 One-Minute NMR: E-Seminar Protocols Press *6 to mute (listen) Press #6 to un-mute (ask a question) As a courtesy to others, please mute your phone line while listening

2. 2 One-Minute NMR 2007 Demo Tour University of Buffalo: Buffalo, NY; January 8 TriMR Regional Meeting: Research Triangle Park, NC; Feb 15-16 Southwest Regional Demonstration, Kalypsys San Diego; March 27 ENC: Daytona Beach, FL; April 22 – 26 Schedule a live demo in your area! Call Protasis for details.

3. 3 One-Minute NMR Winter 2006-07

4. 12 th International Symposium on Marine Products Queenstown, New Zealand, February 2007 58 Speakers and Many Poster Presentations - Sponsors Included Protasis Corporation - Sessions: Discovery Kirk Gustafson, National Cancer Institute, Frederick, MD “Exploring the inherent chemical diversity of marine invertebrates: New compounds from familiar sources” Environment Genome Development Barbara Potts, Neureus, San Diego, CA “Marine microbe derived anti-cancer agents in Phase I clinical trials” New Frontiers William Fenical, Scripps Institute, San Diego, CA “Marine natural products: Where we’ve been and where we’re going”

5. Integrating NMR into Modern Laboratory Workflow The Wellplate and Microvial as Efficient Transport Media Among Automated Analytical Methods  Professor John Blunt University of Canterbury Christchurch, New Zealand  Dr. David Detlefsen Novatia LLC Monmouth Station, NJ USA  Dr. Mike Frey JEOL, Peabody, MA USA  Mark O’Neil-Johnson Sequoia Sciences St. Louis, MO USA Data and Application Examples Courtesy of:

6. 6 HPLC Fract Collect DAD ELSD Vacuum Centrifuge Mass Spec NMR Bioassay Wellplate-Centric Workflow An Example from the University of Canterbury

7. E9,E10,E 11 G4 HPLC with ELSD detection of 600  g extract of unidentified endophytic fungus 250  L/well

8. 500 MHz 8  g in 6  L CD 3 OD PRESAT 1.5 min Recognisable features : 5 CH 3 groups, of which 1 methoxyl, 3 singlet CH 3 -C, 1 doublet CH 3 -C; 1 aldehyde

9. only 1 hit 12

10. 10 MicroFlow NMR Literature www.microNMR.com

11. 11 Your MicroFlow NMR Product Source www.protasis.com