1. - DNA specify all kinds of proteins in the cell - DNA is NOT the direct template for the protein synthesis. There must be intermediate specified by the DNA *The flow of the genetic information DNA transcription RNA translation protein. Structure of RNA 1.Large, unbranched macromolecule, but smaller than DNA 2.Ribose sugar instead deoxy ribose 3.Uracil instead Thymine. 4.Single stranded in most cases.  %G ≠ %C - Complementary to DNA template. - Double helical regions “hairpin loop” DNA Transcription

2. *Types of RNA A.Ribosomal RNA (rRNA) 80% - Component of ribosomes / protein synthesis - Three different sizes of rRNA: 23 S, 16 S, 5 S  Prokaryotic cell, eukaryotic mitochondria - Four different sizes of rRNA: 28 S, 18 S, 5.8 S, 5 S  Eukaryotic cell. B.t-RNA - Smallest RNA (74 – 95 nucleotide) - 15% of total RNA, contains unusual bases. - Act as “adaptor” carries the specific a.a to the site of protein synthesis. C.m-RNA - 5% - Carries the genetic information from DNA to the cytosol where it used as a template for protein synthesis. D.snRNA - Plays role in RNA modification after the transcription in eukaryotic cell

3. Ribonucleases Types of RNA

4. t-RNA, m-RNA, rRNA, snRNA are synthesized by RNA polymerases. RNA synthesis 1.Needs DNA template: RNA polymerase takes instruction from DNA template. 2.Activated precursors ATP, GTP, UTP, CTP. 3.Divalent metallic ion mg +2 RNAn + ribonucleoside triphosphate  RNAn+1 + PPi 4.5’ 3’ synthesis - 3’ – OH makes nucleophilic attack to α-phosphate. - The reaction is driven by hydrolysis of PPi - No need for primer. - No exo- or endo- nuclease activity.

5. t-RNA, m-RNA, rRNA, snRNA are synthesized by RNA polymerases. RNA synthesis 1.Needs DNA template: RNA polymerase takes instruction from DNA template. 2.Activated precursors ATP, GTP, UTP, CTP. 3.Divelent metalic ion mg +2 RNAn + ribonucleoside triphosphate  RNAn+1 + PPi 4.5’ 3’ synthesis

6. - 3’ OH makes nucleophilic attack to α-phosphate. - The reaction is driven by hydrolysis of PPi - No need for primer. - No exo- or endo- nuclease activity.

7. * Transcription in prokaryotes - One type of RNA polymerase. - DNA gene contains promotor sites that specifically bind RNA polymerase and determine where the transcription begins. Strong promotor  Frequent transcription Mutation in the promotor  Impair transcription RNA polymerase is multisubunit enzyme. - Core enzyme 2αββ’ units 5’ 3’ synthesis of RNA. but, lacks the specificty, cannot recognize the promotor region on the DNA template. - Holo enzyme = Core enzyme + σ unit σ Factor enables the polymerase to recognize the promotor region.  (it will be released when transcription starts)

8. DNA Transcription

9. Coding (sense) strand Transcription unit Termination Up stream Down stream 5’3’ - 35- 10 +1 Template strand (anti sense) 3’5’ -10: 5’ TATAAT 3’ (pribnow box) -35: 5’ TTGACA 3’ +1: start site: the first nucleotide of the DNA sequence to be transcripted. eg. Coding template: 5’ GCG ATA TAA TAG 3’ Template strand: 3’ CGC TAT ATT ATC 5’ mRNA : 5’ GCG AUA UAA UAG 3’ Promotor regions in bacterial gene

10. RNA synthesis 1.Initiation RNA polymerase bind to promoter site  unwinds the double helical DNA (about two turns before starting the synthesis) DNA Transcription

12. Transcription Initiation

13. 2.Elongation RNA polymerase catalyzes the formation of phospho diester bond. And it moves unidirectional until the termination signals. - NO need for primer - RNA start with purin (A or G) - σ unit of the holoenzyme is released after the initiation. 3.Termination Elongation continues until a termination process occurs: A.P-Independent termination The transcripted DNA contains stop signals which are palindromic GC rich region followed by AT rich region. DNA Palindrome 5’ CGACTGCAGTCG 3’ 3’ GCTGACGTCAGC 5’ - The sequence on one strand reading 5’ – 3’ is the same as the sequence on the complementary strand reading in the same direction.

14. GC palindromic region stabilizes a secondary structure followed by a string of poly U Stable hair pin slow down the transcription process Poly U bonding to the DNA template is weak Palindrome dependent Termination

16. Hexamer protein Break the RNA- DNA Hybrid by pulling the RNA away RHO (P-factor) protein termination

17. DNA Transcription

18. Transcription of Eukaryotic genes: Transcription of Eukaryotic genes: - Three types of RNA polymerase: 1.RNA polymerase I: large ribosomal RNA (18S, 5.8S, 28S and it’s location in the nucleolus) 2.RNA polymerase II: mRNA precursors and it takes place in nucleoplasm, and (SnRNA) 3.RNA polymerase III: the 5S RNA (nucleoplasm) Mitochondrial RNA polymerase: like as in prokaryotes RNA polymerase in eukaryotes also catalyzes the: - The nucleophilic addition of 3’-OH of the growing RNA chain to the α-phosphat - The 5’ – 3’ addition according to the instruction of anti parallel DNA template - NO need for primer - Lock the nuclease activity RNA polymerase recognize promoter regions in the gene

19. Transcription of Eukaryotic cell

20. (CAAT) box ( TATA) box Transcription unit Termination Start site Coding strandTemplate strand 5’ 3’ 5’ +1 -70-25 Promotor regions in Eukaryotic gene

21. Post transcriptional modification Post transcriptional modification The 1º - transcript is the linear copy of the transcriptional unit. rRNAs are produced from longer precursor. In prokaryotes and eukaryotes cells. - 5S is synthesized by RNA polymerase III - m-RNA in prokaryotes is identical to the 1º - transcript. m-RNA of eukaryotic cells. 1º - transcript (hnRNA) modification m-RNA 1.Capping (translation & stabilizing) 2.Poly A- tail: by polytransferase 3.Removal of Introns Introns: the nucleotide sequences that don’t code for proteins. Exons: the nucleotide sequences that code for proteins.

22. Tail (stabilize & exit) The cap is Added by Guanylyl transferase Post transcriptional modification: capping and Tailing

23. Exon 1 Intron Exon 2 RNA polymerase II Splicing The introns are removed and the remaining exons spliced together to form the mature RNA SnRNA + proteins small nuclear ribonucleo- protein particles -SnRNP- (spliceosomes) Splicing the exon segments The 1º- transcript unmature m-RNA Removal of Introns

24. Post transcriptional modification

25. RNA inhibitors - Some antibiotics prevent cell growth by inhibiting RNA synthesis. Rifampin Inhibits the initiation of transcription by binding to β-subunit of prokaryotic RNA polymerase. Actinomycin D It binds to the DNA template and interferes with movement of RNA polymerase along the DNA (used as anticancer agent) Toxins produced by some fungi e.g. α-Amantin form a complex with RNA polymerase II so inhibits RNA synthesis inhibits protein synthesis.

26. The End