1. Clinical Haematology & Cell Counting
D-Cell 60
Clinical Haematology & Cell Counting
Prepared By
IHSAN M. Said
Dubai
DIAGON® Ltd.

2. CLINICAL HAEMATOLOGY
Clinical haematology plays a primary role in any laboratory. Haematological test are one of the first type ordered by the doctor to determine if his patient has any type of disease
To help you understand haematology we will look at what blood is, how it is best measured and what it looks like under the microscope
Dubai
DIAGON® Ltd.

3. CLINICAL HAEMATOLOGY Various blood cells on a slide
Eosiniphil
Lymphocyte
Monocyte
Neutrophil
Platelet
Basophil
Red Blood Cell
Dubai
DIAGON® Ltd.

4. What is Blood
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Dubai

5. Volumetric % in whole blood
Red Blood Cells
White Blood Cells
Platelets
Normal density
4.5 – 5.5 x1012 cells/l
5 – 10 x109 cells/l
150 – 450 x109 cells/l
Nucleated?
No*
Yes No
Sub-populations
and their %
RBC: 99.9%
*NRBC (Nucleated RBC) 0.1%
GRAnulocytes: 65%
NEUtrophil: 60%
EOSinophil: 4%
BASophil: 1%
LYMphocytes: 30%
MONOcytes: 5%
Shape, size
Biconcave (donut) shape diameter: µm thickness: µm
GRAnulocytes: µm
LYMphocytes: 8-15 µm
MONocytes: µm
Fragments with a
diameter of 2-4 µm
Volume of cell
80 – 100 fl
50 – 1500 fl (native)
5 – 15 fl
Volumetric % in whole blood
45 %
0.1 %
0.3 %
Cell composition of whole human blood
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DIAGON® Ltd.

6. Red Blood Cells Clinical Relevance
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DIAGON® Ltd.

7. White Blood Cells 5 Types
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DIAGON® Ltd.

8. Platelets Clinical relevance
Dubai
DIAGON® Ltd.

9. D-Cell 60 Cell counting principle
White Blood Cell, Red Blood Cell and Platelet are counted and sized by the Electrical Impedance Method.
DIAGON® Ltd.

10. D-Cell 60 Counting Principle electrical Impedance Method
Volumetric Impedance Method
Dubai
DIAGON® Ltd.

11. DIAGON® Ltd.

12. The Counting principle
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A red cell passes through RBC aperture
Red Blood Cell
Sensing Zone
Oscilloscope
Oscilloscope
Dubai

13. Counting principle A White cell passes through WBC aperture Neutrophil
DIAGON® Ltd.
Counting principle
A White cell passes through WBC aperture
Neutrophil
Oscilloscope
Sensing Zone
Oscilloscope
Dubai
DIAGON® Ltd.

14. Applying Thresholds to separate Plt from RBC
20 fl
2 fl
Base line
Platelets
RBC of various
sizes
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DIAGON® Ltd.

15. RBC Histogram Normal Distribution Histogram in Normal samples
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Normal Distribution Histogram in Normal samples

16. RBC histogram 256 channel high resolution RBC histogram
RBC population
256 channel high resolution RBC histogram
display cell population data between 24 and 350 fl
Dubai
DIAGON® Ltd.

17. Platelet Histogram
DIAGON® Ltd.
Normal Log Histogram in Normal samples

18. WBC Histogram
DIAGON® Ltd.
3 Part Diff

19. WBC histogram 256 channel high resolution WBC histogram
DIAGON® Ltd.
WBC histogram
Lymphocyte
Monocyte
Granulocyte
256 channel high resolution WBC histogram
display cell population data between 35 and 450 fl

20. WBC histogram
Mono
Lymp
Neut
Baso
Eos
Dubai
DIAGON® Ltd.

21. D-Cell 60
HGB Measurement Method
Dubai
DIAGON® Ltd.

22. D-Cell 60
Fully automatic 3-part diff
haematology analyser
DIAGON®

23. D-Cell 60 General Introduction: Which parameters are determined?
What are the main features and specifications?
What makes D-CELL 60 convenient for the customer ?
DIAGON®

24. D-Cell 60 Parameters 3 p a r t d i f f , 1 9 p a r a m e t e r s
Measured parameters
RBC, WBC, HGB, MCV, PLT
Derived parameters
LYM#, MON#, GRN#, LYM%, MON%, GRN%
HCT, MCH, MCHC, RDW-CV, RDW-SV
PCT, MPV, PDW
DIAGON®

25. D-Cell 60 Features Measuring system: DIAGON®
Particle counting: Impedance methode
2 channels (RBC+PLT / WBC)
HGB measurement: Photometric methode (525 nm)
photometer incl. in WBC channel
3-part differentiation: derived from histogram
Direct volumetric system for determination of count volume
Throughput: 60 samples / hour
DIAGON®

26. D-Cell 60 Features Sampling system: DIAGON®
Open tube sampling: pipettor system
Sample volume: 13 µl
Pre-dilute mode: for capillary samples
diluent dispensing for 1st dilution step
sample volume: 20 µl
DIAGON®

27. D-Cell 60 Features Reagent system: DIAGON®
Diluent: Diaton-D-Diluent (20 ltr.)
Lyse: Dia-Lyse-D (500 mL)
Detergent: Dia-Rinse-D (5 ltr.)
Enzymatic Cleaner: Dia-Clean-D (100 mL)
Probe-Cleaner: Dia-Probe-Clean-D (50mL)
Fill level monitoring for each reagent container

28. D-Cell 60 Features User Interface: 10“ Colour LCD screen, 256 colours
Results and 3 histograms on one screen
Numeric keyboard with 9 function keys
External keyboard and barcode scanner optional
Built-in thermo or external printer
Data communication port
Large internal memory ( samples with histogram)
DIAGON®

29. D-Cell 60 Features X-B Analysis available (Dr. Brian Bull)
Quality Control
Control Blood data input / edit
9 separate files
Each file stores 31 QC mesurment
Graphical presentation ( 4 par. on one screen )
Table presentation
X-B Analysis available (Dr. Brian Bull)
Monitoring MCH , MCHC and MCV
Graph and Table
DIAGON®

30. D-Cell 60 Features Calibration: Data review DIAGON®
Auto Calibration ( up to 5 measurements )
Manual Calibration
Data review
Several search options ( by name, data, ID …)
Review both results and histograms for each patient
DIAGON®

31. D-Cell 60 Benefits Low sample volume (13 µL ) Low reagent consumption
High analytical precision
Simple operation concept
Efficient Throughput (60 sampls / hour)
Large, bright colour LCD screen
DIAGON®

32. D-Cell 60 Benefits User convenience Powerful data management DIAGON®
Only one key stroke to start measurement
Simple, Windows oriented software structure
Reagent containers with level sensor
Minimized maintenance requirements
Powerful data management
Results and histograms on one screen
results incl. histograms in memory
Efficient QC data management
DIAGON®