1. New Life Science Products 2011 @

2. AppliChem © 2011 2 There is another top address in Darmstadt!

3. AppliChem © 2011 3 There is another top address in Darmstadt! Colorada – Fluorescence Labels starting at page222 Laguna Colorada (Bolivia) Image: Wikipedia

4. AppliChem © 2011 4 There is another top address in Darmstadt! Colorada – Fluorescence Labels wavelength [nm] B: Emission A: Absorption Spectra of Colorada Fluorescent Dyes:

5. AppliChem © 2011 5 There is another top address in Darmstadt! o BDP derivative Dipyrrometheneboron difluoride o Cyanine dye o Coumarine dye o Acenaphthopyrrole dye Colorada – Fluorescence Labels based on 4 chemical classes:

6. AppliChem © 2011 6 There is another top address in Darmstadt! Colorada – Fluorescence Labels Schematic presentation of the product‘s variations: different charges: o anionic o cationic o zwitterionic o neutral

7. AppliChem © 2011 7 There is another top address in Darmstadt! Colorada – Fluorescence Labels

8. AppliChem © 2011 8 There is another top address in Darmstadt! Colorada – Fluorescence Labels different reactive groups: o acid (carboxylic acid, COOH), diacid o azide (N 3 ) o active ester (NHS-ester, COOX) o iodoacetamide o 2-pyridyldisulfideamine (PDA) o aliphatic amine (NH 2 ) o dichlorotriazine (DCT)

9. AppliChem © 2011 9 There is another top address in Darmstadt! Colorada Dye Selection Table Colorada Excitation λ max [nm] Laser Excitation Source Emission λ max [nm] Stoke’s shift [nm] Reactive GroupsQuencher Chemical Class Alternative Dyes 425 N425Diode V47651COOH, COOX, NH2, IODQS35COUMPF415 500 N496Ar, Diode B50610COOH, COOX, NH2, N3, PDAQSY7BDP FITC, FAM, BODIPY FL, AF488, PF488, OG488, PF505, OG514 515 N515Ar, Diode B53924COOH, COOX, NH2, N3, PDAQSY7BDPAF514, 6-JOE, 6-TET, PF520LSS 530 N529Nd:YAG; Diode G56132COOH, COOX, NH2, N3, PDAQSY9BDPBODIPY R6G, AF532 550 C550Nd:YAG; Diode G; HeNe G56717COOH, COOX KIT, NH2, IOD, PDAC5QCYAN TAMRA, BODIPY TMR, CY3, DL549, PF546, AF546, AF555, PF555 550 Z550Nd:YAG; Diode G; HeNe G56616COOH, COOX KIT, NH2, N3, IOD, PDAC5QCYAN TAMRA, BODIPY TMR, CY3, DL549, PF546, AF546, AF555, PF555 550 A553Nd:YAG; Diode G; HeNe G56815COOH, COOX KIT, NH2, IOD, PDAC5QCYAN TAMRA, BODIPY TMR, CY3, DL549, PF546, AF546, AF555, PF555 555 N551HeNe G57212COOH, COOX, NH2, N3, PDAC5QBDPRhodamine R, Cy3B 570 N573Kr161239COOH, COOX, NH2, N3, PDAC5QBDPAF568 577 N577Kr159222COOH, COOX, NH2, N3, PDAQS21ACENCy3.5, PF590 580 N580Kr159222COOH, COOX, NH2, N3, PDAQS21BDPCy3.5, PF590 600 N593HeNe Y; Diode Y62437COOH, COOX, N3, PDAQS21BDP TEXAS RED, BODIPY TR, AF594, AF610, PF 610 630 N629HeNe R, Diode R164617COOH, COOX, N3, PDAQSY21BDPBODIPY 630/650, AF633, PF633, EB 10/30 645 C641HeNe R; Diode R166322COOH, COOX (& KIT), NH2, IOD, PDAQSY21CYAN Cy5, BODIPY 650/665, DL649, AF 647, PF647 645 Z643HeNe R; Diode R166522COOH, COOX KIT, NH2, IOD, PDAQSY21CYAN Cy5, BODIPY 650/665, DL649, AF 647, PF647 645 A648Kr2, HeNe R; DiodeR1-R266721COOH, COOX (& KIT), NH2, N3, IOD, PDAQSY21CYAN Cy5, BODIPY 650/665, DL649, AF 647, PF647 678 C679Kr2, Diode R2-R370728COOH, COOX (KIT), PDAQSY21CYANCy5.5, AF660, AF680, PF680 678 Z678Kr2, Diode R2-R370527COOH, COOX KIT, NH2, N3QSY21CYANCy5.5, AF660, AF680, PF680 678 A677Kr2, Diode R2-R370326COOH, COOX KITQSY21CYANCy5.5, AF660, AF680, PF680 770 C768Diode NIR179022COOH, COOX, DCT, NH2,CYANPF780 770 A772Diode NIR180729COOH, COOX, DCT, NH2CYANPF780 800 C802Diode NIR284038COOH, COOX, DCT, NH2CYAN 800 A802Diode NIR284745COOH, COOX, DCT, NH2CYAN 830 C827Diode NIR386740COOH, COOX, DCT, NH2CYAN 830 A826Diode NIR385125COOH, COOX, DCT, NH2CYAN Cy5, Alexa Fluor 647 (Invitrogen) Cy5, Alexa Fluor 647 (Invitrogen) Cy5, Alexa Fluor 647 (Invitrogen) TAMRA (Invitrogen) TAMRA (Invitrogen) TAMRA (Invitrogen) Cy5.5, Alexa Fluor 680 (Invitrogen) Cy5.5, Alexa Fluor 680 (Invitrogen) Cy5.5, Alexa Fluor 680 (Invitrogen)

10. AppliChem © 2011 10 There is another top address in Darmstadt! Literature Colorada – Fluorescence Labels

11. AppliChem © 2011 11 There is another top address in Darmstadt! Literature request

12. AppliChem © 2011 12 There is another top address in Darmstadt! Applications o Peptide labeling Colorada – Fluorescence Labels o Amino acid labeling o Protein labeling / Antibody labeling o RNA and DNA labeling

13. AppliChem © 2011 13 There is another top address in Darmstadt! Colorada – Fluorescence Labels Merge between a normal plasma labeled with Colorada 645 Z – Iodoacetamide (C5NIASO3) and plasma from a FSGS patient (glomerulosclerosis) labeled with Colorada 550 Z – Iodoacetamide (C3NIASO3). Example:DIGE (Differential Display Gel Electrophoresis), 2-D gel of labeled plasma proteins.

14. AppliChem © 2011 14 There is another top address in Darmstadt! Colorada – Labeling Kits NEW!!!

15. AppliChem © 2011 15 There is another top address in Darmstadt! Colorada – Labeling Kits Step 1 Dye Solution Preparation Step 2 Antibody Solution Preparation

16. AppliChem © 2011 16 There is another top address in Darmstadt! Colorada – Labeling Kits Step 3 Conjugation Procedure Step 4 Isolation of the Conjugate

17. AppliChem © 2011 17 There is another top address in Darmstadt! Colorada – Labeling Kits Product No. * ChromophoreAbs. λ max. Em. λ max. Molar extinction coefficient A9368,0001 A9368,0005 Colorada 550553 nm568 nm150000 M -1 cm -1 A9404,0001 A9404,0005 Colorada 645648 nm667 nm250000 M -1 cm -1 A9517,0001 A9517,0005 Colorada 678677 nm703 nm195000 M -1 cm -1 Colorada Antibody Labeling Kits *1 or 5 labeling reactions of 1 mg protein each

18. AppliChem © 2011 18 There is another top address in Darmstadt! Colorada – Labeling Kits Product No. * ChromophoreAbs. λ max. Em. λ max. Molar extinction coefficient A9368,0001 A9368,0005 Colorada 550553 nm568 nm150000 M -1 cm -1 A9404,0001 A9404,0005 Colorada 645648 nm667 nm250000 M -1 cm -1 A9517,0001 A9517,0005 Colorada 678677 nm703 nm195000 M -1 cm -1 Product No. ** ChromophoreAbs. λ max. Em. λ max. Cyanin structure Molar extinction coefficient A9426,0001 A9426,0005 Colorada 490 DIGE 484 nm498 nmCy2110000 M -1 cm -1 A9447,0001 A9447,0005 Colorada 550 DIGE 548 nm567 nmCy3150000 M -1 cm -1 A9498,0001 A9498,0005 Colorada 645 DIGE 641 nm663 nmCy5250000 M -1 cm -1 Colorada Antibody Labeling Kits Colorada DIGE Protein Labeling Kits *10 or 50 labeling reactions *1 or 5 labeling reactions of 1 mg protein each

19. AppliChem © 2011 19 There is another top address in Darmstadt! Literature soon available Colorada – Labeling Kits

20. AppliChem © 2011 20 There is another top address in Darmstadt! PBS Buffer tablets NEW!!!

21. AppliChem © 2011 21 There is another top address in Darmstadt! Phosphate buffered saline (PBS) 1. powder PBS buffer (10X Dulbecco's) - Powder A0965 PBS buffer (1X Dulbecco's) - Powder A1908 PBS buffer (1X Dulbecco's) - Powder A0964 2. solution PBS / Tween® - SolutionA3814 Blocking solution, e.g. in ELISA

22. AppliChem © 2011 22 There is another top address in Darmstadt! pH 7,4 for final volumes of: 100 ml, 200 ml, 500 ml, and 1 L pH 7,2 for a final volume of: 1 L Phosphate buffered saline (PBS) On popular demand: new at AppliChem 3. PBS Buffer tablets

23. AppliChem © 2011 23 There is another top address in Darmstadt! Transfection GFP Drosophila image: Univ of California, San Diego website AppliFect – Transfection Reagents starting at page 114

24. AppliChem © 2011 24 There is another top address in Darmstadt!  Transfection Reagents nucleus lipoplex cell membrane endosome DNA entry during Mitosis cytosol DNA cationic lipid co-lipids release of DNA endocytosis ! !

25. AppliChem © 2011 25 There is another top address in Darmstadt!  Transfection Reagents AppliFect – fast and easy protocol 1. seed cells 2. prepare DNA-solution + AppliFect 3. complex 4. incubate 5. reporter gen assay

26. AppliChem © 2011 26 There is another top address in Darmstadt! Liposomes consisting of polycationic transfection reagent + a neutral co-lipid AppliFect - for routine transfection of plasmid DNA into eukaryotic cells o easy - ready-to-use reagent o effective - highest expression rates o robust - serum doesn‘t interfere with transfection  Transfection Reagents

27. AppliChem © 2011 27 There is another top address in Darmstadt! 1. AppliFect  Transfection Reagents AppliFect example: COS-7 cells GFP transfected transmitted light

28. AppliFect example: COS-7 cells GFP transfected fluorecence channel

29. AppliFect example: COS-7 cells GFP transfected overlay

30. AppliChem © 2011 30 There is another top address in Darmstadt! o Lipofectamine (Invitrogen) 672,00 €/1 ml [German list price] o FuGENE HD (Promega) 369,00 €/1 ml [German list price] o GeneJuice (Novagen) 306,00 €/1 ml [German list price] o Metafectene (Biontex) o Unifectin (Novagen) o Ribojuice (Novagen) o Nanofectin (Novagen) Competitors  Transfection Reagents AppliFect - key benefits: o High efficiency o Competitive Price

31. AppliChem © 2011 31 There is another top address in Darmstadt! o Lipofectamine (Invitrogen) 672,00 €/1 ml [German list price] o FuGENE HD (Promega) 369,00 €/1 ml [German list price] o GeneJuice (Novagen) 306,00 €/1 ml [German list price] o Metafectene (Biontex) o Unifectin (Novagen) o Ribojuice (Novagen) o Nanofectin (Novagen) Competitors  Transfection Reagents AppliFect - key benefits: o High efficiency o Competitive Price 188,40 €/1 ml [German list price]

32. AppliChem © 2011 32 There is another top address in Darmstadt! 4. AppliFect SI  Transfection Reagents 3. AppliFect Insect- DNA for insect cells 2. AppliFect LowTox- pDNA, for sensitive cells 232,00 €/1 ml [German list price] 1. AppliFect - (plasmid) pDNA, RNA - for siRNA, miRNA, RNA

33. AppliChem © 2011 33 There is another top address in Darmstadt!  Transfection Reagents Promote AppliFect o literature: application notes comprehensive product information o promotions o sampling