1. Genomics Eddy Rubin December 3, 2008

2. DOE Joint Genome Mission Supported by the DOE Office of Science, JGI unites the expertise of five national laboratories—Lawrence Berkeley, Lawrence Livermore, Los Alamos, Oak Ridge, and Pacific Northwest—along with the Hudson Alpha Institute for Biotechnology to advance genomics in support of the DOE missions related to clean energy generation and environmental characterization and cleanup. JGI is operated by the University of California for the U.S. Department of Energy. The JGI Production Genomics Facility (PGF) is located in Walnut Creek, CA, equidistant between LBNL & LLNL. 2

3. The JGI Production Genomics Facility (PGF) The PGF’s workforce of ~240 is comprised of LBNL & LLNL employees (80%/20%). 3

4. JGI: A National User Facility World Class Science: –Advancing the scientific literature—over 48 papers in the journals Science and the Nature where JGI played a leadership role (first or senior authorship) –over the last three years, JGI has played a role and shared co-authorship in a total of 234 peer-reviewed publications Efficient and Productive Sequencing : –Generating DNA sequence data (in October 2008 alone) of over 20 billion nucleotides, or the equivalent of over six human genomes). 4

5. JGI as National User Facility II Broad/Diverse Impact through the Community Sequencing Program— over 1350 Users WW in 2008 5

6. Genomics: A Workplace of Many Disciplines: Production, Computation, and Research @ the PGF: –Repetitive manual manipulation of sample plates to facilitate the Sanger sequencing process –Sample prep for next gen (454/Illumina) sequencers –Standard microbiological/molecular biology lab practice –Keyboard-intensive development of computational tools/data management/analysis @ Building 84/74 (LBNL): –Standard molecular biology laboratory practices –Micromanipulation of mouse embryos for the creation of transgenic models 6

7. Integrated Safety Managment (ISM) Implementation JGI ISM Plan Weekly Senior Management Safety Briefings Safety presentations at All Hands Meetings Management Safety Area Walk Throughs Department heads participate in the Employee Safety Committee Department Heads assigned as responsible persons for CATS Issues 7

8. JGI (Genomics) Practices Enhanced Safety Internal Ergonomic Program –Dedicated Staff Ergonomist –Early Intervention/monitoring program –Routine Case meetings –Enhanced Remedy Interactive program –On site LBNL Registered Nurse Custom Safety Training Courses –PGF-0010 JGI Safety Briefing –PGF -0057 Laboratory Ergonomics Participation in Employees Led Safety –Employee Safety Committee –JGI Emergency Response Team –Safety Culture Team –Ergonomics Working Group 8 Ergonomics Working Group Emergency Response Team