1. Group II Aleksandra Tkacz UST Lidia Proczek UST Katarzyna Michalów UST Jana Hruba’ STU Eva Kuzielova’ STU Krzysztof Burek UST ‘ Nanoparticles and biochemistry’

2. 1.Nanomaterials and quantum dots (QDs) 2.Biochemistry 3.Gd 2 O 3 :Tb 3+ @SiO x system 4.Researches 5.Summary Outline

3. Nanoparticles

4. Quantum Dots (QDs) Nanometre-scale semiconductor crystals

6. Quantum Dots (QDs) How it works...

7. Quantum Dots (QDs) Relationship between crystal size and colour The smaller the size, the shorter the wavelenght

8. Quantum Dots (QDs) Advantages in comparison with conventional Dyes -QDs emit light at a variety of precise wavelenghts -QDs can be excited by any wavelenght shorter than the one it emits -QDs have wide range of avaible colours -QDs are extremely stable

9. Quantum Dots (QDs) Ways of obtaining nanoparticles -Sol-gel -Plasma -Mechanochemical -Aerosol processing -High-energy ball milling -Polyol and other chemical -Hydrothermal processing -Other chemical and physical techniques as needed

10. Quantum Dots (QDs) Applications

11. Life science Controling the size and phase of semiconductor nanocrystals by biological materials (peptides, etc.) QDs as a markers

14. Gd 2 O 3 :Tb 3+ @SiO x [1] [1] Chem. Mater. 2005, 17, 1673-1682 Double Luminescence for Biological Labeling

15. Optical properties of Gd 2 O 3 :Tb 3 Double Luminescence for Biological Labeling -high photostability -narrow emission bands

16. Gd 2 O 3 :Tb 3+ @SiO x system The role of the layer : Gd 2 O 3 :Tb 3+ @SiO x system The role of the layer : Double Luminescence for Biological Labeling -allows the dispersion of the particles in aqueous solution without loss of luminescence intensivity -the luminescence of polisiloxane-coated Gd 2 O 3 nanoparticles is more intense than that in the case of the naked core Gd 2 O 3 -in the presence of amino groups, organic dyes and biotargeting groups (nucleic acid, biotin, streptavid) were covalently linked to the polisiloxane network

17. Research work ‘Preparation and characterization of ZnS:Mn nanoparticles’

18. Materials SubstanceCompanyPurity Zinc AcetateSIGMA - ALDRICH98% Mangan AcetateROTH98% ;) Sodium SulphideRIEDEL-DE-HEAN60 – 62 % CysteaminMERCK97 % Ethanol-Technical Acetic Acid-Technical

19. Preparation route I

20. Preparation route II

21. Results a) b) pH=4,82 pH=4,91

22. Results a) b) pH=4,82 pH=4,91

23. Results a) b) pH=4,82 pH=4,91

24. Summary -A wide range of future application -QDs have better properties than conventional Dyes -The luminescence of QDs @ SiO x systems is very intense -It’s not difficult to obtain luminescent nanoparticles

26. References 1)C. Louis, R. Bazzi, C.A. Marquette, J-L. Bridot, S.Roux, G. Ledoux, B. Mercier, L. Blum, P. Perriat, O. Tillement Chem. Mater 2005, 17, 1673-1682 2)E. Klarreich Nature 2001, 413, 450-452 3)N.G. Portney, K. Singh, S. Chaudhary, G. Destito, A.Schneemann, M.Machester, M. Ozkan Langmuir 2005, 21, 2098-2103 4)C.E. Flynn, C. Mao, A. Hayhurst, J.L. Williams, G. Georgiu, B. Iverson, A.M. Belcher J.Mater. Chem. 2005, 13, 2414-2421