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Matthew Mancuso BEE 7600, Professor Dan Luo Department of Biomedical Engineering, Cornell University Presented Thursday March 31 st, 2011 Nucleic Acids.

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Presentation on theme: "Matthew Mancuso BEE 7600, Professor Dan Luo Department of Biomedical Engineering, Cornell University Presented Thursday March 31 st, 2011 Nucleic Acids."— Presentation transcript:

1 Matthew Mancuso BEE 7600, Professor Dan Luo Department of Biomedical Engineering, Cornell University Presented Thursday March 31 st, 2011 Nucleic Acids & Nanoparticles in the Luo Lab

2 Points to Cover 1.) Patterning by Controlled Evaporation 2.) Free-Standing Nanoparticle Superlattices 3.) Nanocrystal Characterization

3 DNA-Nanoparticle Structures: Different Crystals 1.) So what’s the big deal with DNA? How does it help us make nanostructures when combined with Goldnanoparticles? The Bottom Line: Base pair matching allows DNA-NP structures to be highly specific. GNPs allow for the creation of structures with vastly different material properties. C. A. Mirkin, R. L. Letsinger, R. C. Mucic, and J. J. Storhoff, "A DNA-based method for assembling nanoparticles into macroscopic materials," Nature 382, 607-609 (1996) Alivisatos, A. P.; Johnsson, K. P.; Peng, X. G.; Wilson, T. E.; Loweth, C. J.; Bruchez, M. P.; Schultz, P. G., Organization of 'nanocrystal molecules' using DNA. Nature 1996, 382, (6592), 609-611. 199 6

4 Nanopatterning via Microdroplets W.L. Cheng, N.Y. Park, M.T. Walter, M.R. Hartman, D. Luo, Nanopatterning self-assembled nanoparticle superlattices by moulding microdroplets, Nature Nanotechnology 3 (2008) 682-690.

5 Nanopatterning via microdroplets W.L. Cheng, N.Y. Park, M.T. Walter, M.R. Hartman, D. Luo, Nanopatterning self-assembled nanoparticle superlattices by moulding microdroplets, Nature Nanotechnology 3 (2008) 682-690.

6 Free-Standing Nanoparticle Superlattices W.L. Cheng, M.J. Campolongo, J.J. Cha, S.J. Tan, C.C. Umbach, D.A. Muller, D. Luo, Free-standing nanoparticle superlattice sheets controlled by DNA, Nature Materials 8 (2009) 519-525. W.L. Cheng, M.J. Campolongo, S.J. Tan, D. Luo, Freestanding ultrathin nano-membranes via self assembly, Nano Today 4 (2009) 482-493.

7 Free-Standing Ultra-thin Nanoparticle Membranes FUN! Membranes W.L. Cheng, M.J. Campolongo, J.J. Cha, S.J. Tan, C.C. Umbach, D.A. Muller, D. Luo, Free-standing nanoparticle superlattice sheets controlled by DNA, Nature Materials 8 (2009) 519-525. W.L. Cheng, M.J. Campolongo, S.J. Tan, D. Luo, Freestanding ultrathin nano-membranes via self assembly, Nano Today 4 (2009) 482-493.

8 30,000 Smaller!70,000 Heavier! Other FUN Membranes R. Vendamme, S.-Y. Onoue, A. Nakao and T. Kunitake, Nat. Mater. 5 (2006)

9 FUN Membranes

10 DNA-Capped Nanoparticle Assembly

11 Free-Standing Nanoparticle Superlattices

12

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