1. ANGIOSPERM REPRODUCTION AND BIOTECHNOLOGY
CHAPTER 38
ANGIOSPERM REPRODUCTION AND BIOTECHNOLOGY

2. Male wasps transfer pollen to an Mediterranean orchid

3. I. Concept 38.1: Unique Features of the Angiosperm Life Cycle
A. Overview
Diploid (2n) sporophytes produce spores by meiosis; these grow into haploid (n) gametophytes
Gametophytes produce haploid (n) gametes by mitosis; fertilization of gametes produces a sporophyte
In angiosperms, the sporophyte is the dominant generation, the large plant that we see
The gametophytes are reduced in size and depend on the sporophyte for nutrients
The angiosperm life cycle is characterized by “three Fs”: flowers, double fertilization, and fruits

4. Angiosperm Life Cycle

5. B. Flower Structure and Function
Flowers are the reproductive shoots of the angiosperm sporophyte; they attach to a part of the stem called the receptacle
The receptacle or base is a modified stem end which holds the flower
2. Flowers consist of four floral organs: sepals, petals, stamens, and carpels
3. Sepals are the green leaf-like floral parts which protect the flower in the bud stage
All the sepals form the calyx
4. Petals are the conspicuous colored flower parts
All the petals together form the corolla

6. A stamen consists of a filament topped by an anther with pollen sacs that produce pollen (male)
6. A carpel has a long style with a stigma on which pollen may land (female)
At the base of the style is an ovary containing one or more ovules which develop into seeds after fertilization
8. A single carpel or group of fused carpels is called a pistil
9. Complete flowers contain all four floral organs
10.Incomplete flowers lack one or more floral organs, for example stamens or carpels
11.Clusters of flowers are called inflorescences

8. Flower Structure

9. Lilies

10. Microspore nucleus undergoes mitosis to produce
C. Development of Male Gametophytes in Pollen Grains (Microsporogenesis)
Within pollen sacs of an anther, diploid microsporocytes undergo meiosis to form 4 haploid microspores
Microspore nucleus undergoes mitosis to produce
a generative cell and a tube cell
Wall of the microspore thickens and becomes
sculptured into a species-specific pattern
The generative cell and tube cell are enclosed
in the thickened wall to form the pollen grain,
an immature male gametophyte

11. Tube cell will form the pollen tube
Generative cell forms 2 sperm nuclei (mature male gametophyte)

13. D. Development of Female Gametophytes (Embryo Sacs) (Megasporogenesis)
Megasporocyte in ovule grows and goes through meiosis to form 4 haploid megaspores
(only 1 usually survives)
Remaining megaspore grows and its nucleus
undergoes 3 mitotic divisions, forming
1 large cell with 8 haploid nuclei
Membranes partition this into a multicellular embryo sac

14. Ovules form within ovary and contain the female gametophyte or embryo sac
At the end containing the egg is the micropyle (an opening through the integuments surrounding the embryo sac)

16. E. Pollination
In angiosperms, pollination is the transfer of pollen from an anther to a stigma
Pollination can be by wind, water, bee, moth and butterfly, fly, bird, bat, or water
As pollen germinates, tube nucleus forms a pollen tube down the style toward the embryo sac
At the same time, generative nucleus divides by mitosis to form 2 sperm nuclei (male gamete)
Pollen tube penetrates micropyle

19. Pollen Tube

20. F. Double Fertilization
After landing on a receptive stigma, a pollen grain produces a pollen tube that extends between the cells of the style toward the ovary
Double fertilization results from the discharge of two sperm from the pollen tube into the embryo sac
One sperm fertilizes the egg, and the other combines with the polar nuclei, giving rise to the triploid (3n) food-storing endosperm
After double fertilization, each ovule develops into a seed
The ovary develops into a fruit enclosing the seed(s)

21. Double Fertilization

22. Sperm Nuclei Enter Micropyle

23. G. Seed Development, Form and Function
1. Endosperm Development
Endosperm development usually precedes embryo development
In most monocots and some eudicots, endosperm stores nutrients that can be used by the seedling
In other eudicots, the food reserves of the endosperm are exported to the cotyledons

24. 2. Embryo Development
The first mitotic division of the zygote is transverse, splitting the fertilized egg into a basal cell and a terminal cell
Mitosis continues to occur, eventually producing cotyledons and apical meristems

26. 3. Structure of the Mature Seed
The embryo and its food supply are enclosed by a hard, protective seed coat
The seed enters a state of dormancy (during the last stages of maturation)
In some eudicots the embryo consists of the embryonic axis attached to two cotyledons (seed leaves)
Below the cotyledons the embryonic axis is called the hypocotyl and terminates in the radicle (embryonic root); above the cotyledons it is called the epicotyl (shoot tip with a pair of miniature leaves)

27. Eudicot Seed

29. A monocot embryo has one cotyledon
Grasses, such as maize and wheat, have a special cotyledon called a scutellum which absorbs nutrients from the endosperm during germination
Two sheathes enclose the embryo of a grass seed: a coleoptile covering the young shoot and a coleorhiza covering the young root

30. Monocot Seed

31. 4. Seed Dormancy
Seed dormancy increases the chances that germination will occur at a time and place most advantageous to the seedling
The breaking of seed dormancy often requires environmental cues, such as temperature or lighting changes

32. 5. Seed Germination and Seedling Development
Germination depends on imbibition, the uptake of water due to low water potential of the dry seed
The radicle (embryonic root) emerges first
Next, the shoot tip breaks through the soil surface
In many eudicots, a hook forms in the hypocotyl, and growth pushes the hook above ground
The hook straightens and pulls the cotyledons and shoot tip up
In maize and other grasses, which are monocots, the coleoptile pushes up through the soil

33. Eudicot Germination

34. Monocot Germination

35. H. Fruit Form and Function
A fruit develops from the ovary
It protects the enclosed seeds and aids in seed dispersal by wind or animals
A fruit may be classified as dry, if the ovary dries out at maturity, or fleshy, if the ovary becomes thick, soft, and sweet at maturity
Fruits are also classified by their development:
-Simple, a single or several fused carpels
-Aggregate, a single flower with multiple separate carpels
-Multiple, a group of flowers called an inflorescence
5. An accessory fruit contains other floral parts in addition to ovaries
6. Fruit dispersal mechanisms include: water, wind, animals

40. II. Concept 38.2: Plants Reproduce Sexually, Asexually, or Both
A. Asexual reproduction, or vegetative reproduction, produces clones. B. Mechanisms of Asexual Reproduction 1. Fragmentation is an example in which pieces of the parent plant break off to form new individuals that are exact genetic replicas of the parent. 2. Apomixis is the asexual production of seeds from a diploid cell. (Ex: dandelions)

41. C. Agriculture uses several techniques of artificial vegetative reproduction such as grafting, growing clones from cuttings, and test-tube cloning
D. Asexual reproduction can be beneficial to a successful plant in a stable environment
E. Sexual reproduction generates genetic variation that makes evolutionary adaptation possible
F. While some flowers self-fertilize, other have methods to prevent self-fertilization and maximize genetic variation.
One of these is self-incompatibility, in which a plant rejects its own pollen or that of a closely related plant, thus insuring cross-pollination