1. The Cell Cycle Chapter 12

2. Modeling the Cell Cycle in a Normal Cell Objective: model the events and controls of the cell cycle. At your station… ▫A large Ziploc bag with materials ▫Cell cycle wheel ▫Scissors ▫Tape ▫Dried lima beans

3. How do these paper chromosomes compare to real chromosomes?

4. First, become familiar with the chromosomes that will be used in your cell model. Your cell has 2 pairs of chromosomes. These are made from blue and pink paper. Each pair is the same length. Find the 2 pairs. Throughout this simulation, each person in the group will be responsible for one of the chromosomes. Each pair of chromosomes is made up of 2 partners, a maternal chromosome and a paternal chromosome.

5. During this simulation, how would you tell the difference between the maternal and paternal chromosomes?

6. The chromosomes are found inside your cell that is represented by the small Ziploc bag labeled “Cell.” Place your 2 pairs of chromosomes into the small Ziploc bag.

7. The remainder of the materials will be used throughout the activity and should be kept in the large bag labeled “Materials”, as a supply/synthesis area.

8. Lets Begin! A cell next to your cell has died. This cell’s death causes an external growth signal and tells your cell to begin the cell cycle.

9. Turn Wheel to G1 Phase During G1 phase, the cell grows in size and synthesizes mRNA and proteins that are required for DNA synthesis. Please double the mass of your cell using the lima beans.

10. Turn Wheel to First Checkpoint At this checkpoint a growth regulator protein, called Ras cyclin, checks that cells are big enough to enter the next part of the cell cycle. Raise your hand so that Ras cyclin (your teacher) may check the mass of your cell.

11. Why is it important to check that the cell is big enough to continue with the cell cycle?

12. Turn Wheel to Next Checkpoint DNA in these chromosomes can be damaged by a number of agents including radiation, toxic chemicals, and free radicals. At this checkpoint, another protein known as p53 will inspect the chromosomes’ DNA for damage. Raise your hand to have p53 check your DNA for damage.

13. What do you think would happen if this p53 surveillance system did not work properly?

14. Turn Wheel to the S Phase During this part of the cell cycle, DNA must be replicated. Using the materials from the supply bag, make exact copies of each of your DNA strands. Place the centromere regions of the chromatids on top of each other.

15. Turn Wheel to Next Checkpoint A protein complex called ATM/ Nibrin inspects to make sure that the DNA was copied without mistakes. Raise your hand to have ATM/Nibrin (your teacher) check your replicated DNA for damage.

16. Why is it important to check the replicated DNA?

17. Turn Wheel to G2 Phase During this part of the cell cycle, the cell prepares for mitosis by synthesizing the materials it will need. Pull the string out of large bag.

18. G1, S, and G2 of Interphase used to be called the “resting phase”. Why is resting phase not a good name?

19. Move Wheel to Mitosis During Prophase, DNA in the chromosomes condenses and coils. Wrap each chromatid around a pencil and then remove the pencil. Attach your two coiled chromatids together by placing the centromeres on top of each other.

20. During Metaphase, the chromosomes line up in the center of the cell. Place one set of spindle fibers at each side (pole) of your desk (cell). ▫Attach one spindle fiber from the left side of your desk to the centromere on one chromatid. ▫Attach a spindle fiber from the right side of your desk to the centromere on the other chromatid.

21. Turn Wheel to Next Checkpoint A protein called MAD1 checks to be sure the spindle fibers have attached properly. Spindle fibers need to be attached correctly so that chromosomes are distributed evenly to the new cells. Raise your hand to have MAD1 check your spindle attachment.

22. Continue with Mitosis Proceed to Anaphase by pulling on the spindle apparatus to separate chromatids at the centromere. Complete Telophase by removing the spindle from the chromosomes. Place the spindle fibers back in the supply bag.

23. Turn Wheel to Cytokinesis Cut your small Ziploc bag, labeled “CELL” in half. Place the chromosomes from the left side of your desk into one half of the bag, and the chromosomes from the right side of your desk in the other half. Distribute the mass of the original cell to the daughter cells. Tape the open ends of the cut Ziploc bag closed. You should now have two smaller cells.

24. Compare the two daughter cells.