1. MBV2010/BIO2140 Colloquium, February 5

2. RULES Multiple choice Only one correct answer 30-60 seconds to answer No textbook, computer, mobile phone, please There will be a prize for the group with the most correct answers.

3. Example 4789 x 9548 = ?? A 44 877 267 B 42 872 186 C 45 725 372 D 49 693 726 0

4. Example 4789 x 9548 = ?? 0

5. Nucleic acids were first isolated in the … A 17 th century B 18 th century C 19 th century D 20 th century 1

6. Nucleic acids were first isolated in the … 1 Friedrich Miescher

7. The major form of DNA in cells is … A A-DNA B B-DNA C C-DNA D Z-DNA 2

8. The major form of DNA in cells is … 2

9. A A-DNA B B-DNA C C-DNA D Z-DNA 3 Which form of DNA is shown here ?

10. 3

11. Most RNA in cells is … A messenger RNA B transfer RNA C micro RNA D ribosomal RNA 4

12. Most RNA in cells is … 4

13. Transcriptomes consist of … A messenger RNA B transfer RNA C ribosomal RNA D all of the above 5

14. Transcriptomes consist of … 5

15. The genetic code A consists of 62 codons B has 3 stop codons C is used in transcription D specifies 25 amino acids 6

16. The genetic code 6

17. Restriction endonucleases A are located in the nucleus of cells B are sequence-specific C were discovered in the 1980s D are present in bacteria and fungi 7

18. Restriction endonucleases 7

19. Which compound is not found in nucleic acids? A Pyridines B Purines C Phosphate D Ribose 8

20. Which compound is not found in nucleic acids? 8 PyridinePyrimidine

21. The secondary structure of proteins is stabilized by … A Ionic bonds B Hydrogen bonds C Disulfide bonds D Hydrophobic interactions 9

22. The secondary structure of proteins is stabilized by … 9

23. DNA polymerases A synthesize DNA in 3’ -> 5’ direction B require a primer to initiate DNA synthesis C require ATP D bind only to DNA 10

24. DNA polymerases 10

25. The human nuclear genome … A is split into 24 chromosomes B Is the largest genome known to date C Contains about 2 billion bp D Contains about 40,000 genes 11

26. The human nuclear genome … 11 Human female karyogram

27. Reverse transcriptases A Synthesize RNA B Use DNA as template C Are viral enzymes D Are present in all eukaryotic cells 12

28. Reverse transcriptases 12

29. ORF scanning A is used to find intron sequences B is used to find functional genes C is used to identify protein-coding genes D is used to find intergenic sequences 13

30. ORF scanning 13

31. A codon bias … A is present in intergenic regions of a genome B is used to find protein- coding genes C is only present in eukaryotic genomes D has been found for all codons 14

32. A codon bias … 14

33. Expression of genes can be analyzed by A northern analysis B Southern analysis C Comparative genomics D PCR 15

34. Expression of genes can be analyzed by 15

35. An alpha-helix A is a DNA structure B is a protein structure C winds to the left D is stabilized by base stacking 16

36. An alpha-helix 16

37. ß-sheets are stabilized by A hydrophobic bonds B ionic bonds C hydrogen bonds D all of the above 17

38. ß-sheets are stabilized by 17