1. OSM v.s. ISM1-BA M. Engin Enginsu M.D., Ph.D. KadıköyŞifa Hastanesi İstanbul Turkey

2. KŞ Hospital IVF unit is a private clinic 600 cycles per year ISM 1 – BA is the only culutre medium used in the clinic for over 3 years The average age of the patients is 35,98 >40 is %32,6

3. Protocols ISM 1 – BA Oocyte collection in Universal IVF untill ICSI Transfer to ISM1 untill D2 Change to BA at 42-48 hrs Blast development Change BA on 72-78 hrs never disturb on D4 Transfer in BA OSM Oocyte collection in OS Fert untill ICSI Transfer to OS Clev after ICSI Refresh Os Clev on 22-26 hrs Change to OM Blas on 72-78 hrs or ET Blast development never disturb on D4 Transfer in OS Blas

4. Day 2 Embryos ISM1 BAOSM

5. Day 3 Embryos ISM1 BAOSM

6. Blastocysts ISM1 BAOSM

7. Overall Results n = 76 n = 114

8. Overall Results

10. Good Prognosis Group 3 oocytes

13. Blastocyst Results

15. THAW ET

16. Conclusion On basis of fertilization, cleavage and embryo development rate OSM and ISM1- BA are comparable Blastocyts development is significantly better in OSM Clinical pregnancy rates are significantly better in OSM This may be the result of longer exposure of the embryos to Hyaluronan