1. Chapter 7: The Cell Membrane

2. Overview: Life at the Edge Plasma membrane- the boundary that separates the living cell from its surroundings The plasma membrane exhibits selective permeability, allowing some substances to cross it more easily than others

3. Structure of the Plasma Membrane Phospholipids are the most abundant lipid in the plasma membrane Made up of a hydrophillc phosphate group (head) and 2 hydrophobic fatty acid chains (tails)

4. Structure of the Plasma Membrane Phospholipids are amphipathic molecules, containing hydrophobic and hydrophilic regions Fluid Mosaic model- membrane is a fluid structure with a “mosaic” of various proteins embedded in it

5. Phospholipid Bilayer 2 layers of phospholipids Hydrophobic tails face away from fluid Hydrophilic heads face toward fluid

6. Fig. 7-2 Hydrophilic head WATER Hydrophobic tail WATER

7. The Fluidity of Membranes Phospholipids can move within the bilayer Most of the lipids, and some proteins, drift laterally

8. Fig. 7-5 Lateral movement (~10 7 times per second) Flip-flop (~ once per month) (a) Movement of phospholipids (b) Membrane fluidity Fluid Viscous Unsaturated hydrocarbon tails with kinks Saturated hydro- carbon tails (c) Cholesterol within the animal cell membrane Cholesterol

9. Fig. 7-5b (b) Membrane fluidity Fluid Unsaturated hydrocarbon tails with kinks Viscous Saturated hydro- carbon tails

10. Fig. 7-5c Cholesterol (c) Cholesterol within the animal cell membrane

11. Fig. 7-7 Fibers of extracellular matrix (ECM) Glyco- protein Microfilaments of cytoskeleton Cholesterol Peripheral proteins Integral protein CYTOPLASMIC SIDE OF MEMBRANE Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate

12. Peripheral proteins are bound to the surface of the membrane Integral proteins penetrate the hydrophobic core (integrated into membrane) Integral proteins that span the membrane are called transmembrane proteins Proteins have hydrophobic and hydrophilic regions Proteins in the Membrane!

13. Six major functions of membrane proteins: –Transport –Enzymatic activity –Signal transduction –Cell-cell recognition –Intercellular joining –Attachment to the cytoskeleton and extracellular matrix (ECM)

14. Fig. 7-9 (a) Transport ATP (b) Enzymatic activity Enzymes (c) Signal transduction Signal transduction Signaling molecule Receptor (d) Cell-cell recognition Glyco- protein (e) Intercellular joining (f) Attachment to the cytoskeleton and extracellular matrix (ECM)

15. The Role of Membrane Carbohydrates in Cell-Cell Recognition Cells recognize each other by binding to surface molecules, often carbohydrates, on the plasma membrane Membrane carbohydrates may be covalently bonded to lipids (forming glycolipids) or more commonly to proteins (forming glycoproteins)

16. Movement of Molecules A cell must exchange materials with its surroundings, a process controlled by the plasma membrane Plasma membranes are selectively permeable, regulating the cell’s molecular traffic

17. Transport Proteins Transport proteins allow passage of hydrophilic substances across the membrane Some transport proteins, called channel proteins, have a hydrophilic channel that certain molecules or ions can use as a tunnel Channel proteins called aquaporins facilitate the passage of water

18. Other transport proteins, called carrier proteins, bind to molecules and change shape to shuttle them across the membrane A transport protein is specific for the substance it moves Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

19. Diffusion Diffusion – movement of molecules from an area of high concentration to an area of low concentration until equilibrium is reached Molecule movement is random, but tend to experience a net movement in one direction

20. Fig. 7-11 Molecules of dye Membrane (cross section) WATER Net diffusion Equilibrium (a) Diffusion of one solute Net diffusion Equilibrium (b) Diffusion of two solutes

21. Substances diffuse down their concentration gradient, the difference in concentration of a substance from one area to another NO ENERGY IS NEEDED to move substances down the concentration gradient The diffusion of a substance across a biological membrane is passive transport because it requires no energy from the cell to make it happen Concentration Gradient

22. (b) Diffusion of two solutes Fig. 7-11b Net diffusion Equilibrium

23. Osmosis Osmosis is the diffusion of water across a selectively permeable membrane Water diffuses across a membrane from the region of lower solute concentration to the region of higher solute concentration

24. Lower concentration of solute (sugar) Fig. 7-12 H2OH2O Higher concentration of sugar Selectively permeable membrane Same concentration of sugar Osmosis

25. Water Balance of Cells Without Walls Tonicity is the ability of a solution to cause a cell to gain or lose water Isotonic solution: Solute concentration is the same as that inside the cell; no net water movement across the plasma membrane Hypertonic solution: Solute concentration is greater than that inside the cell; cell loses water Hypotonic solution: Solute concentration is less than that inside the cell; cell gains water

26. Hypotonic solution (a ) Animal cell (b ) Plant cell H2OH2O Lysed H2OH2O Turgid (normal) H2OH2O H2OH2O H2OH2O H2OH2O Normal Isotonic solution Flaccid H2OH2O H2OH2O Shriveled Plasmolyzed Hypertonic solution

27. Hypertonic or hypotonic environments create osmotic problems for organisms Osmoregulation, the control of water balance, is a necessary adaptation for life in such environments The protist Paramecium, which is hypertonic to its pond water environment, has a contractile vacuole that acts as a pump

28. Filling vacuole 50 µm (a) A contractile vacuole fills with fluid that enters from a system of canals radiating throughout the cytoplasm. Contracting vacuole (b) When full, the vacuole and canals contract, expelling fluid from the cell.

29. Water Balance of Cells with Walls Cell walls help maintain water balance A plant cell in a hypotonic solution swells until the wall opposes uptake; the cell is now turgid (firm) If a plant cell and its surroundings are isotonic, there is no net movement of water into the cell; the cell becomes flaccid (limp), and the plant may wilt

30. In a hypertonic environment, plant cells lose water; eventually, the membrane pulls away from the wall, a usually lethal effect called plasmolysis Plasymosis

31. Facilitated Diffusion Facilitated diffusion is still passive because the solute moves down its concentration gradient Some transport proteins, however, can move solutes against their concentration gradients

32. Facilitated Diffusion: Passive Transport Aided by Proteins In facilitated diffusion, transport proteins speed the passive movement of molecules across the plasma membrane Channel proteins provide “channels” that allow a specific molecule or ion to cross the membrane Channel proteins include –Aquaporins, for facilitated diffusion of water –Ion channels that open or close in response to a stimulus (gated channels)

33. Fig. 7-15 EXTRACELLULAR FLUID Channel protein (a) A channel protein Solute CYTOPLASM Solute Carrier protein (b) A carrier protein

34. Carrier proteins undergo a subtle change in shape, then will carry the solute across the membrane (glucose)

35. Active Transport Active transport moves substances against their concentration gradient (area of low concentration to high concentration) Active transport requires energy, in the form of ATP Active transport is performed by specific proteins embedded in the membranes

36. 2 EXTRACELLULAR FLUID [Na + ] high [K + ] low [Na + ] low [K + ] high Na + CYTOPLASM ATP ADP P Na + P 3 K+K+ K+K+ 6 K+K+ K+K+ 5 4 K+K+ K+K+ P P 1 Fig. 7-16-7

37. Fig. 7-17 Passive transport Diffusion Facilitated diffusion Active transport ATP

38. Sodium Potassium Pump http://highered.mcgraw- hill.com/sites/0072495855/stude nt_view0/chapter2/animation__ how_the_sodium_potassium_pu mp_works.html

39. Bulk Transport Large molecules, such as polysaccharides and proteins, cross the membrane in bulk via vesicles Bulk transport requires energy

40. Exocytosis In exocytosis, transport vesicles migrate to the membrane, fuse with it, and release their contents Many secretory cells use exocytosis to export their products

41. Endocytosis In endocytosis, the cell takes in macromolecules by forming vesicles from the plasma membrane There are three types of endocytosis: –Phagocytosis (“cellular eating”) –Pinocytosis (“cellular drinking”) –Receptor-mediated endocytosis

42. In phagocytosis a cell engulfs a particle in a vacuole The vacuole fuses with a lysosome to digest the particle

43. Fig. 7-20 PHAGOCYTOSIS EXTRACELLULAR FLUID CYTOPLASM Pseudopodium “Food”or other particle Food vacuole PINOCYTOSIS 1 µm Pseudopodium of amoeba Bacterium Food vacuole An amoeba engulfing a bacterium via phagocytosis (TEM) Plasma membrane Vesicle 0.5 µm Pinocytosis vesicles forming (arrows) in a cell lining a small blood vessel (TEM) RECEPTOR-MEDIATED ENDOCYTOSIS Receptor Coat protein Coated vesicle Coated pit Ligand Coat protein Plasma membrane A coated pit and a coated vesicle formed during receptor- mediated endocytosis (TEMs) 0.25 µm

44. Fig. 7-20a PHAGOCYTOSIS CYTOPLASM EXTRACELLULAR FLUID Pseudopodium “Food” or other particle Food vacuole Food vacuole Bacterium An amoeba engulfing a bacterium via phagocytosis (TEM) Pseudopodium of amoeba 1 µm

45. In pinocytosis, molecules are taken up when extracellular fluid is “gulped” into tiny vesicles

46. Fig. 7-20b PINOCYTOSIS Plasma membrane Vesicle 0.5 µm Pinocytosis vesicles forming (arrows) in a cell lining a small blood vessel (TEM)

47. Fig. 7-UN1 Passive transport: Facilitated diffusion Channel protein Carrier protein

48. Fig. 7-UN2 Active transport: ATP

49. Fig. 7-UN3 Environment: 0.01 M sucrose 0.01 M glucose 0.01 M fructose “Cell” 0.03 M sucrose 0.02 M glucose

50. Fig. 7-UN4