1. Some mt & cp proteins contain subunits encoded by organelle’s genome

2. Plant mtDNA encodes ~13 proteins, also rRNA & tRNA subunits of ATP synthase & complexes I, II, III & IV some mRNA are trans-spliced from 2 diff transcripts! some mRNA are edited: bases changed after synthesis!

3. Plant mtDNA mtDNA recombines to form new genes, some poison pollen development to create cytoplasmic male sterility Pollen don't transmit mito!

4. CMS A long noncoding RNA regulates photoperiod-sensitive male sterility, an essential component of hybrid rice(2012) doi: 10.1073/pnas.1121374109 A non-coding RNA locus mediates environment-conditioned male sterility in rice. (2012) Cell Research 22:791–792. doi:10.1038/cr.2012.43 Comparative expression profiling of miRNA during anther development in genetic male sterile and wild type cotton. (2013) BMC Plant Biology 13:66 Differential Proteomic Analysis of Anthers between Cytoplasmic Male Sterile and Maintainer Lines in Capsicum annuum L.(2013) Int. J. Mol. Sci. 14(11), 22982-22996; doi:10.3390/ijms14112298210.3390/ijms141122982 Transcriptome map of plant mitochondria reveals islands of unexpected transcribed regions (2011) BMC Genomics 12: 279. Heterozygous alleles restore male fertility to cytoplasmic male-sterile radish (Raphanus sativus L.): a case of overdominance(2013) J. Exp. Bot. 64: 2041-2048.

5. CMS have major increase in respiration and # mitochondria after meiosis 40 x increase in mt/ cell in tapetum 20x in sporogenous cells Often arise due to recombination creating weird fusion encoding defective protein: poisons respiration Not enough energy to make good pollen

6. CMS RF genes often encode protein which restores good mRNA eg. by splicing fusion in Hong-Lian cms rice Or by blocking WA352 expression in Wild Abortive CMS rice Constant battle: mito evolve way to kill pollen and nucleus evolves way to overcome it.

7. Mitochondria and the immune system Mitochondria are involved in many aspects of innate immunity Pathogens and damaged mito trigger very similar responses pathogen-associated molecular patterns (PAMPs) are bound by pattern-recognition receptors (PRRs) that trigger inflammatory responses damage-associated molecular patterns (DAMPs) released by broken mito bind the same receptors & trigger same responses.

8. Mitochondria and the immune system Mitochondria are involved in many aspects of innate immunity Mito play important role in recognizing & fighting viruses Via RLR (retinoic acid-inducible receptors) pathway that detects dsRNA

9. Mitochondria and the immune system Mito play important role in recognizing & fighting viruses Via RLR (retinoic acid-inducible receptors) pathway that detects dsRNA MAVS ( mitochondrial antiviral signaling) protein on MOM is key

10. Mitochondria and the immune system Mito play important role in recognizing & fighting viruses Via RLR (retinoic acid-inducible receptors) pathway that detects dsRNA MAVS ( mitochondrial antiviral signaling) protein on MOM is key dsRNA receptors bind MAVS & trigger interferon & cytokine synthesis

11. Mitochondria and the immune system MAVS ( mitochondrial antiviral signaling) protein on MOM is key dsRNA receptors bind MAVS & trigger interferon & cytokine Synthesis Viruses also interact with mt in countless specific ways

12. Mitochondria and the immune system Viruses also interact with mt in countless specific ways Many involve blocking Programmed Cell Death pathways

13. Mitochondria and cell death Cells die many ways, but mito are often involved

14. Programmed cell death vs necrosisNecrosis: progressive loss of membrane integrity swelling of cytoplasm, release of cell constituents Often follows irreversible injury Passive IndiscriminatePCD Active Orderly process mediated by intracellular death programs May or may not be due to an external factor Nuclear condensation Condensation of PM

15. Types of programmed cell death Apoptosis Autophagy Pyroptosis Ferroptosis Plant programmed cell death Necroptosis

16. Apoptosis Occurs as normal part of development e.g. patterning of hands/feet

17. Apoptosis Occurs as normal part of development Ordered process that breaks cell into easily recycled pieces

18. Apoptosis Occurs as normal part of development Is also triggered by many kinds of damage Especially to DNA Many cancer cells do not commit apoptosis

19. Apoptosis Occurs as normal part of development Ordered process that breaks cell into easily recycled pieces Caspases digest proteins

20. Apoptosis Ordered process that breaks cell into easily recycled pieces Caspases digest proteins CAD digests DNA

21. Apoptosis Occurs as normal part of development Two basic steps: commitment and execution

22. Apoptosis Occurs as normal part of development Two basic steps: commitment and execution Commitment depends on interplay between various signals Bax & Bcl2 have opposite effects

23. Apoptosis Two basic steps: commitment and execution Commitment depends on interplay between various signals Bax & Bcl2 have opposite effects 2 main pathways: extrinsic & intrinsic