1. Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings PowerPoint ® Lecture Presentations for Biology Eighth Edition Neil Campbell and Jane Reece Lectures by Chris Romero, updated by Erin Barley with contributions from Joan Sharp Chapter 7 Membrane Structure and Function

2. Learning Targets: 1.Define the following terms: amphipathic molecules, aquaporins, diffusion, osmosis. 2.Explain how membrane fluidity is influenced by temperature and membrane composition 3.Distinguish between the following pairs or sets of terms: peripheral and integral membrane proteins; channel and carrier proteins; osmosis, facilitated diffusion, and active transport; hypertonic, hypotonic, and isotonic solutions Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

3. 4.Explain how transport proteins facilitate diffusion 5.Explain how large molecules are transported across a cell membrane Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings Learning Targets:

4. Cellular membranes are: the boundary that separates the living cell from its surroundings selective permeability, allowing some substances to cross it more easily than others made of Phospholipids and Protein Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

5. Hydrophilic head WATER Hydrophobic tail WATER Phospholipids are amphipathic molecules, containing hydrophobic and hydrophilic regions The Structure of Membranes

6. Phospholipid bilayer Hydrophobic regions of protein Hydrophilic regions of protein Fluid mosaic model (fluid structure with a “mosaic” of various proteins embedded in it)

7. Lateral movement (  10 7 times per second) Flip-flop (  once per month) The Fluidity of Membranes Phospholipids in the plasma membrane can move within the bilayer Most of the lipids, and some proteins, drift laterally

8. As temperatures cool, membranes switch from a fluid state to a solid state Membranes rich in unsaturated fatty acids are more fluid that those rich in saturated fatty acids Membranes must be fluid to work properly; they are usually about as fluid as salad oil Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings The Fluidity of Membranes

9. Fluid Unsaturated hydrocarbon tails with kinks Viscous Saturated hydro- carbon tails The Fluidity of Membranes

10. The steroid cholesterol has different effects on membrane fluidity at different temperatures At warm temperatures (such as 37°C), cholesterol restrains movement of phospholipids At cool temperatures, it maintains fluidity by preventing tight packing Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings The Fluidity of Membranes

11. Cholesterol Cholesterol within the animal cell membrane

12. Membrane Proteins and Their Functions A membrane is a collage of different proteins embedded in the fluid matrix of the lipid bilayer Proteins determine most of the membrane’s specific functions Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

13. Fig. 7-7 Fibers of extracellular matrix (ECM) Glyco- protein Microfilaments of cytoskeleton Cholesterol Peripheral proteins Integral protein CYTOPLASMIC SIDE OF MEMBRANE Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate

14. Peripheral proteins are bound to the surface of the membrane Integral proteins penetrate the hydrophobic core Integral proteins that span the membrane are called transmembrane proteins Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings Membrane Proteins and Their Functions

15. (1) Transport (2) Enzymatic activity (3) Signal transduction ATP Enzymes Signal transduction Signaling molecule Receptor Six major functions of membrane proteins:

16. (4) Cell-cell recognition Glyco- protein (5) Intercellular joining (6) Attachment to the cytoskeleton and extracellular matrix (ECM) Six major functions of membrane proteins:

17. Cell-Cell Recognition Cells recognize each other by binding to surface molecules, often carbohydrates, on the plasma membrane Membrane carbohydrates may be covalently bonded to lipids (forming glycolipids) or more commonly to proteins (forming glycoproteins) Carbohydrates on the external side of the plasma membrane vary among species, individuals, and even cell types in an individual Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

18. Plasma membranes allow cells to exchange materials with its surroundings are selectively permeable, regulating the cell’s molecular traffic Hydrophobic (nonpolar) molecules, such as hydrocarbons, can dissolve in the lipid bilayer and pass through the membrane rapidly Polar molecules, such as sugars, do not cross the membrane easily Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

19. Transport Proteins Allow passage of hydrophilic substances across the membrane Channel proteins, channel certain molecules/ions through membrane Aquaporins facilitate the passage of water Carrier proteins, bind to molecules and change shape to shuttle them across the membrane is specific for the substance it moves Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

20. Passive transport is diffusion of a substance across a membrane with no energy investment Diffusion is the tendency for molecules to spread out evenly into the available space At dynamic equilibrium, as many molecules cross one way as cross in the other direction Animation: Membrane Selectivity Animation: Membrane Selectivity Animation: Diffusion Animation: Diffusion Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

21. Molecules of dyeMembrane (cross section) WATER Net diffusion Equilibrium Substances diffuse down their concentration gradient, the difference in concentration of a substance from one area to another without energy

22. Net diffusion Equilibrium The diffusion of a substance across a biological membrane is passive transport because it requires no energy from the cell to make it happen

23. Effects of Osmosis on Water Balance Osmosis is the diffusion of water across a selectively permeable membrane Water diffuses across a membrane from the region of lower solute concentration to the region of higher solute concentration Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

24. Lower concentration of solute (sugar) H2OH2O Higher concentration of sugar Selectively permeable membrane Same concentration of sugar Osmosis

25. Water Balance of Cells Without Walls Tonicity is the ability of a solution to cause a cell to gain or lose water Isotonic solution: Solute concentration is the same as that inside the cell; no net water movement across the plasma membrane Hypertonic solution: Solute concentration is greater than that inside the cell; cell loses water Hypotonic solution: Solute concentration is less than that inside the cell; cell gains water Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

26. Hypotonic solution Animal cell Plant cell H2OH2O Lysed H2OH2O Turgid (normal) H2OH2O H2OH2O H2OH2O H2OH2O Normal Isotonic solution Flaccid H2OH2O H2OH2O Shriveled Plasmolyzed Hypertonic solution

27. the control of water balance Hypertonic or hypotonic environments create osmotic problems for organisms The protist Paramecium, which is hypertonic to its pond water environment, has a contractile vacuole that acts as a pump Video: Chlamydomonas Video: Chlamydomonas Video: Paramecium Vacuole Video: Paramecium Vacuole Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings Osmoregulation

28. Filling vacuole 50 µm A contractile vacuole fills with fluid that enters from a system of canals radiating throughout the cytoplasm. Contracting vacuole When full, the vacuole and canals contract, expelling fluid from the cell.

29. Water Balance of Cells with Walls Cell walls help maintain water balance A plant cell in a hypotonic solution swells until the wall opposes uptake; the cell is now turgid (firm) If a plant cell and its surroundings are isotonic, there is no net movement of water into the cell; the cell becomes flaccid (limp), and the plant may wilt Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

30. Video: Plasmolysis Video: Plasmolysis Video: Turgid Elodea Video: Turgid Elodea Animation: Osmosis Animation: Osmosis In a hypertonic environment, plant cells lose water; eventually, the membrane pulls away from the wall, a usually lethal effect called plasmolysis Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings Water Balance of Cells with Walls

31. Facilitated Diffusion: transport proteins speed the passive movement of molecules across the plasma membrane Channel proteins provide corridors that allow a specific molecule or ion to cross the membrane Channel proteins include – Aquaporins, for facilitated diffusion of water – Ion channels that open or close in response to a stimulus (gated channels) Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

32. EXTRACELLULAR FLUID Channel protein A channel protein Solute CYTOPLASM Solute Carrier protein A carrier protein

33. Carrier proteins undergo a subtle change in shape that translocates the solute-binding site across the membrane Some diseases are caused by malfunctions in specific transport systems, for example the kidney disease cystinuria Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

34. Active transport uses energy to move solutes against their gradients Facilitated diffusion is still passive because the solute moves down its concentration gradient Some transport proteins, however, can move solutes against their concentration gradients Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

35. Active Transport moves substances against their concentration gradient requires energy, usually in the form of ATP performed by specific proteins embedded in the membranes Animation: Active Transport Animation: Active Transport Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

36. EXTRACELLULAR FLUID [Na + ] high [K + ] low Na + [Na + ] low [K + ] high CYTOPLASM Cytoplasmic Na + binds to the sodium-potassium pump. 1 Sodium-potassium pump is a type of active transport system Allows cells to maintain concentration gradients that differ from their surroundings

37. Na + binding stimulates phosphorylation by ATP. Na + ATP P ADP 2 Na + P 3 Phosphorylation causes the protein to change its shape. Na + is expelled to the outside.

38. K + binds on the extracellular side and triggers release of the phosphate group. P P K+K+ K+K+ 4 K+K+ K+K+ Loss of the phosphate restores the protein’s original shape. 5

39. K + is released, and the cycle repeats. K+K+ K+K+ 6

40. Passive transport Diffusion Facilitated diffusion Active transport ATP

41. Ion Pumps Maintain Membrane Potential Membrane potential is the voltage difference across a membrane Voltage is created by differences in the distribution of positive and negative ions Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

42. Two combined forces drive the diffusion of ions across a membrane: 1. A chemical force (the ion’s concentration gradient) 2. An electrical force (the effect of the membrane potential on the ion’s movement) Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings Electrochemical gradient

43. An electrogenic pump is a transport protein that generates voltage across a membrane The sodium-potassium pump is the major electrogenic pump of animal cells The main electrogenic pump of plants, fungi, and bacteria is a proton pump Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

44. Proton pump EXTRACELLULAR FLUID H+H+ H+H+ H+H+ H+H+ Proton pump + + + H+H+ H+H+ + + H+H+ – – – – ATP CYTOPLASM –

45. Cotransport occurs when active transport of a solute indirectly drives transport of another solute Plants commonly use the gradient of hydrogen ions generated by proton pumps to drive active transport of nutrients into the cell Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

46. Proton pump – – – – – – + + + + + + ATP H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ Diffusion of H + Sucrose-H + cotransporter Sucrose Cotransport

47. Bulk transport Small molecules and water enter or leave the cell through the lipid bilayer or by transport proteins Large molecules, such as polysaccharides and proteins, cross the membrane in bulk via vesicles Bulk transport requires energy Occurs by exocytosis and endocytosis Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

48. Exocytosis transport vesicles migrate to the membrane, fuse with it, and release their contents Many secretory cells use exocytosis to export their products Animation: Exocytosis Animation: Exocytosis Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

49. Endocytosis the cell takes in macromolecules by forming vesicles from the plasma membrane a reversal of exocytosis, involving different proteins There are three types of endocytosis: – Phagocytosis (“cellular eating”) – Pinocytosis (“cellular drinking”) – Receptor-mediated endocytosis Animation: Exocytosis and Endocytosis Introduction Animation: Exocytosis and Endocytosis Introduction Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

50. PHAGOCYTOSIS CYTOPLASM EXTRACELLULAR FLUID Pseudopodium “Food” or other particle Food vacuole Food vacuole Bacterium An amoeba engulfing a bacterium via phagocytosis (TEM) Pseudopodium of amoeba 1 µm Animation: Phagocytosis Animation: Phagocytosis In a cell engulfs a particle in a vacuole The vacuole fuses with a lysosome to digest the particle

51. Plasma membrane Vesicle 0.5 µm Pinocytosis vesicles forming (arrows) in a cell lining a small blood vessel (TEM) molecules are taken up when extracellular fluid is “gulped” into tiny vesicles Pinocytosis Animation: Pinocytosis Animation: Pinocytosis

52. Binding of ligands to receptors triggers vesicle formation A ligand is any molecule that binds specifically to a receptor site of another molecule Animation: Receptor-Mediated Endocytosis Animation: Receptor-Mediated Endocytosis Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings Receptor-mediated endocytosis

53. RECEPTOR-MEDIATED ENDOCYTOSIS Receptor Coat protein Coated pit Ligand Coat protein Plasma membrane 0.25 µm Coated vesicle A coated pit and a coated vesicle formed during receptor- mediated endocytosis (TEMs)

54. Environment: 0.01 M sucrose 0.01 M glucose 0.01 M fructose “Cell” 0.03 M sucrose 0.02 M glucose

56. Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings. If a marine algal cell is suddenly transferred from seawater to freshwater, the algal cell will initially A.lose water and decrease in volume. B.stay the same: neither absorb nor lose water. C.absorb water and increase in volume.

57. Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings. If a marine algal cell is suddenly transferred from seawater to freshwater, the algal cell will initially A.lose water and decrease in volume. B.stay the same: neither absorb nor lose water. C.absorb water and increase in volume.