1. www.boldsystems.org BARCODE OF LIFE DATA SYSTEMS (BOLD) Riadul Mannan Biodiversity Institute of Ontario

2. www.BOLDSYSTEMS.org

3. 1 Architect, 2 Hardware Engineers 4 Programmers, 4 Data Managers BOLD SYSTEMS 450 CPUs, 100TB storage Generator power Remote backup

4. Barcode Records for Fungi, Protists, Plants and Animals 834.5Kspecimens, 69.6K species, 2.1K institutions : 5.1K users

5. Features of BOLD Quality Assessments Primer Registry Alignment Viewer/Editor Integrated Help Taxon ID Tree Distance Summary Sequence Composition Nearest Neighbour Summary Accumulation Curves Multi-Marker ID Engine Bibliography Support Web Service API Workflow *AnalyticsIntegration

6. Specimen Tissue Sample Extract DNA PCR Amplify Sequence Photograph Collection Data Web-Accessible Data and DNA Barcode The Analytical Chain

7. Specimen Taxonomy Identifier IDs Institution Collectors Desc. Lat/Lon Country Collection Lab Data Trace Primers Audit Trail Marker 1 DNA Marker 2 Markers 3 Caption Metadata Copyright Image/Video Literature BOLD Data Model

8. BOLD Multi-Marker Data Tag Cloud of markers Total Markers on BOLD : 45

9. Project Management

10. Data Management SpecimenSequence

11. Data Management 12 3 4

12. Primer Registry Total Primer – 633 Public – 325 Private – 308

13. Data Analysis & Visualization

14. Sequence Analysis - Taxon ID Tree

15. Alignment Browser Features:  Flexible labeling  Variability of positions  Mapping amino acids Future Features:  Highlighting diagnostic position  Mapping to secondary structures  Web based sequence editing

16. Identification System

17. Managing Lab Data - LIMS

18. LOCATING SAMPLES Owner Sample Type Sampleids Processids Keywords Microplate Label Sample Lookup

19. Controlling Liquid Handling Robots

20. PCR Success Report Gel image Sliced by system

21. Management And Monitoring

22. LIMS - Non Lab User

23. Data Publication