1. 1 SURVEY OF BIOCHEMISTRY Course Review

2. 2 PRS-1 Catalytic efficiency is given by what expression? 1.k cat 2.k cat /K M 3.V max /K M 4.[E] T x k cat

3. 3 PRS-1 Catalytic efficiency is given by what expression? 1.k cat 2.k cat /K M 3.V max /K M 4.[E] T x k cat

4. 4 PRS-2 Which amino acid is an alpha-helix breaker? 1.Y 2.W 3.V 4.P

5. 5 PRS-2 Which amino acid is an alpha-helix breaker? 1.Y 2.W 3.V 4.P

6. 6 PRS-3 Amino acids are linked by what types of bonds in proteins? 1.Disulfide bonds 2.Peptide bonds 3.Both #1 and #2 4.None of the above

7. 7 PRS-3 Amino acids are linked by what types of bonds in proteins? 1.Disulfide bonds 2.Peptide bonds 3.Both #1 and #2 4.None of the above

8. 8 PRS-4 Which cofactors are required for the aldolase reaction? 1.NAD + 2.CoA 3.FAD 4.None

9. 9 PRS-4 Which cofactors are required for the aldolase reaction? 1.NAD + 2.CoA 3.FAD 4.None

10. 10 PRS-5 Which of the following is NOT an example of a cofactor? 1.Glucose 2.Thiamine Pyrophosphate 3.Pyridoxal Phosphate 4.Coenzyme A

11. 11 PRS-5 Which of the following is NOT an example of a cofactor? 1.Glucose 2.Thiamine Pyrophosphate 3.Pyridoxal Phosphate 4.Coenzyme A

12. 12 PRS-6 What function does glucagon exhibit? 1.Increasing glycogen breakdown 2.Binding to receptors on liver cells 3.Triggering increased levels of cAMP production 4.All of the above

13. 13 PRS-6 What function does glucagon exhibit? 1.Increasing glycogen breakdown 2.Binding to receptors on liver cells 3.Triggering increased levels of cAMP production 4.All of the above

14. 14 PRS-7 Pyruvate carboxylase converts ___ to _____. 1.Acetyl CoA to pyruvate 2.Pyruvate to acetyl Coa 3.Pyruvate to Oxaloacetate 4.Pyruvate to Phosphoenolpyruate

15. 15 PRS-7 Pyruvate carboxylase converts ___ to _____. 1.Acetyl CoA to pyruvate 2.Pyruvate to acetyl Coa 3.Pyruvate to Oxaloacetate 4.Pyruvate to Phosphoenolpyruate

16. 16 PRS-8 A point mutation in which a purine is replaced by a pyrimidine is called: 1.Transversion 2.Transition 3.Insertion 4.Deletion

17. 17 PRS-8 A point mutation in which a purine is replaced by a pyrimidine is called: 1.Transversion 2.Transition 3.Insertion 4.Deletion See Section 25-4 From Reading - not mentioned in lecture…

18. 18 PRS-9 Which statement is true? 1.Hemoglobin binding of CO 2 stabilizes the T form 2.The sickle cell form of hemoglobin contains a point mutation (E->V) 3.2,3-Bisphosphoglycerate decreases hemoglobin’s affinity for O 2 4.All of the above

19. 19 PRS-9 Which statement is true? 1.Hemoglobin binding of CO 2 stabilizes the T form 2.The sickle cell form of hemoglobin contains a point mutation (E->V) 3.2,3-Bisphosphoglycerate decreases hemoglobin’s affinity for O 2 4.All of the above

20. 20 PRS-10 Which of the following is a unit of O 2 pressure? 1.mm Hg 2.mol/L 3.% 4.nm

21. 21 PRS-10 Which of the following is a unit of O 2 pressure? 1.mm Hg 2.mol/L 3.% 4.nm

22. 22 PRS-11 See Figure 1. The shift from blue to red indicates: 1.An increase in pH 2.A decrease in pH 3.An increase in hemoglobin concentration 4.A decrease in hemoglobin concentration

23. 23 PRS-11 See Figure 1. The shift from blue to red indicates: 1.An increase in pH 2.A decrease in pH 3.An increase in hemoglobin concentration 4.A decrease in hemoglobin concentration

24. 24 PRS-12 Which are 5-C monosaccharides? 1.Glucose and ribose 2.Ribose and fructose 3.Ribose and mannose 4.None of the combinations above

25. 25 PRS-12 Which are 5-C monosaccharides? 1.Glucose and ribose 2.Ribose and fructose 3.Ribose and mannose 4.None of the combinations above

26. 26 PRS-13 Which reaction is an example of an acid-base catalyzed reaction? 1.Hexokinase 2.Pyruvate kinase 3.Phosphoglucose isomerase 4.Pyruvate dehydrogenase

27. 27 PRS-13 Which reaction is an example of an acid-base catalyzed reaction? 1.Hexokinase Nucleophilic attack 2.Pyruvate kinase Nucleophilic attack 3.Phosphoglucose isomerase 4.Pyruvate dehydrogenase 5-step Reaction

28. 28 PRS-14 Which of the following is not a step in the pyruvate dehydrogenase mechanism? 1.Decarboxylation 2.Reduction of FAD to FADH 2 3.Oxidation of NADH to NAD + 4.Production of acetyl-CoA

29. 29 PRS-14 Which of the following is not a step in the pyruvate dehydrogenase mechanism? 1.Decarboxylation 2.Reduction of FAD to FADH 2 3.Oxidation of NADH to NAD + 4.Production of acetyl-CoA

30. 30 Pyruvate Dehydrogenase Rxn

31. 31 PRS-15 Which of the following is not a disulfide reducing agent? 1.Cysteine 2.Beta-mercaptoethanol 3.Lipoic acid 4.Linoleic acid

32. 32 PRS-15

33. 33 PRS-16 cDNA _____. 1.Is DNA found in the cytosol of a cell. 2.Comes from reverse transcription of mRNA. 3.Stands for “capped” DNA. 4.Contains the DNA sequence from the entire genome.

34. 34 PRS-16 cDNA _____. 1.Is DNA found in the cytosol of a cell. 2.Comes from reverse transcription of mRNA. 3.Stands for “capped” DNA. 4.Contains the DNA sequence from the entire genome.

35. 35 PRS-17 Which are nonpolar amino acids? 1.Gly, Val, Lys 2.Arg, Leu, Met 3.Ile, Pro, Phe 4.Ala, Met, Ser

36. 36 PRS-17 Which are nonpolar amino acids? 1.Gly, Val, Lys 2.Arg, Leu, Met 3.Ile, Pro, Phe 4.Ala, Met, Ser

37. 37 PRS-18 Beta sheets can contain _____. 1.Antiparallel strands 2.Parallel strands 3.Interstrand H-bonds 4.All of the above.

38. 38 PRS-18 Beta sheets can contain _____. 1.Antiparallel strands 2.Parallel strands 3.Interstrand H-bonds 4.All of the above.

39. 39 PRS-19 The hydrophobic effect is driven by ______. 1.Enthalpy 2.Entropy 3.The concentration of water 4.Temperature

40. 40 PRS-19 The hydrophobic effect is driven by ______. 1.Enthalpy 2.Entropy 3.The concentration of water 4.Temperature

41. 41 PRS-20 Which is a major component of brain lipids? 1.Fatty acids 2.Triglycerides 3.Gangliosides 4.Phospholipids

42. 42 PRS-20 Which is a major component of brain lipids? 1.Fatty acids 2.Triglycerides 3.Gangliosides 4.Phospholipids

43. 43 Reminders Final exam is closed-book. Bring a calculator. Study by: –Reading the text –Reviewing suggested HW problems –Reviewing What You Should Know summaries (no more added) –Reviewing past exams –Studying lecture notes

44. 44 CETL Course Survey(s) Course-Instructor Opinion Survey (CIOS) –http://www.coursesurvey.gatech.edu –Also on T-Square Home Page Pilot Survey –Go to T-Square: Announcements for link to survey –Focus groups to learn more about student opinions –Deadline: August 3, 2008 –IRB Approval requested: minimal risk