1. Overview: Life at the Edge The plasma membrane is the boundary that separates the living cell from its surroundings The plasma membrane exhibits selective permeability, allowing some substances to cross it more easily than others Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

2. Phospholipids are the most abundant lipid in the plasma membrane Phospholipids are amphipathic molecules, containing hydrophobic and hydrophilic regions The fluid mosaic model states that a membrane is a fluid structure with a “mosaic” of various proteins embedded in it Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

3. Fig. 7-2 Hydrophilic head WATER Hydrophobic tail WATER

4. Fig. 7-3 Phospholipid bilayer Hydrophobic regions of protein Hydrophilic regions of protein

5. The Fluidity of Membranes Phospholipids in the plasma membrane can move within the bilayer Most of the lipids, and some proteins, drift laterally Rarely does a molecule flip-flop transversely across the membrane Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

6. Fig. 7-5 Lateral movement (~10 7 times per second) Flip-flop (~ once per month) (a) Movement of phospholipids (b) Membrane fluidity Fluid Viscous Unsaturated hydrocarbon tails with kinks Saturated hydro- carbon tails (c) Cholesterol within the animal cell membrane Cholesterol

7. Membrane Proteins and Their Functions A membrane is a collage of different proteins embedded in the fluid matrix of the lipid bilayer Proteins determine most of the membrane’s specific functions Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

8. Fig. 7-7 Fibers of extracellular matrix (ECM) Glyco- protein Microfilaments of cytoskeleton Cholesterol Peripheral proteins Integral protein CYTOPLASMIC SIDE OF MEMBRANE Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate

9. Fig. 7-9 (a) Transport ATP (b) Enzymatic activity Enzymes (c) Signal transduction Signal transduction Signaling molecule Receptor (d) Cell-cell recognition Glyco- protein (e) Intercellular joining (f) Attachment to the cytoskeleton and extracellular matrix (ECM)

10. The Role of Membrane Carbohydrates in Cell-Cell Recognition Cells recognize each other by binding to surface molecules, often carbohydrates, on the plasma membrane Membrane carbohydrates may be covalently bonded to lipids (forming glycolipids) or more commonly to proteins (forming glycoproteins) Carbohydrates on the external side of the plasma membrane vary among species, individuals, and even cell types in an individual Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

11. Review How is a phospholipid amphipathic? – Phosphate group is hydrophilic; fatty acids are hydrophobic What is meant by membrane fluidity? – Phospholipids and proteins can shift laterally – they are not fixed How would unsaturated phospholipids in a membrane differ from saturated phospholipids? – Unsaturated would be more fluid due to the kink in the fatty acid chain (C=C) making a more fluid membrane

12. Review What is the primary function of carbohydrates in a membrane? – Cell identification What is the primary function of cholesterol in a membrane? – Stability List some functions of membrane proteins. – Transport, enzyme, signal transduction, recognition, intercellular joining, attachment to cytoskeleton and ECM

13. Can you label this?

14. How did you do?