1. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings PowerPoint Lectures for Biology, Seventh Edition Neil Campbell and Jane Reece Lectures by Chris Romero Chapter 43 The Immune System

2. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Overview: Reconnaissance, Recognition, and Response An animal must defend itself – From the many dangerous pathogens it may encounter in the environment Two major kinds of defense have evolved that counter these threats – Innate immunity and acquired immunity

3. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Innate immunity – Is present before any exposure to pathogens and is effective from the time of birth – Involves nonspecific responses to pathogens Figure 43.1 3m3m

4. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Acquired immunity, also called adaptive immunity – Develops only after exposure to inducing agents such as microbes, toxins, or other foreign substances – Involves a very specific response to pathogens

5. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings A summary of innate and acquired immunity INNATE IMMUNITY Rapid responses to a broad range of microbes ACQUIRED IMMUNITY Slower responses to specific microbes External defensesInternal defenses Skin Mucous membranes Secretions Phagocytic cells Antimicrobial proteins Inflammatory response Natural killer cells Humoral response (antibodies) Cell-mediated response (cytotoxic lymphocytes) Invading microbes (pathogens) Figure 43.2

6. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Concept 43.1: Innate immunity provides broad defenses against infection A pathogen that successfully breaks through an animal’s external defenses – Soon encounters several innate cellular and chemical mechanisms that impede its attack on the body

7. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings External Defenses Intact skin and mucous membranes – Form physical barriers that bar the entry of microorganisms and viruses Certain cells of the mucous membranes produce mucus – A viscous fluid that traps microbes and other particles

8. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings In the trachea, ciliated epithelial cells – Sweep mucus and any entrapped microbes upward, preventing the microbes from entering the lungs Figure 43.3 10  m

9. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Secretions of the skin and mucous membranes – Provide an environment that is often hostile to microbes Secretions from the skin – Give the skin a pH between 3 and 5, which is acidic enough to prevent colonization of many microbes – Also include proteins such as lysozyme, an enzyme that digests the cell walls of many bacteria

10. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Internal Cellular and Chemical Defenses Internal cellular defenses – Depend mainly on phagocytosis Phagocytes, types of white blood cells – Ingest invading microorganisms – Initiate the inflammatory response

11. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Pathogen Pin Macrophage Chemical signals Capillary Phagocytic cells Red blood cell Blood clotting elements Blood clot Phagocytosis Fluid, antimicrobial proteins, and clotting elements move from the blood to the site. Clotting begins. 2 Chemical signals released by activated macrophages and mast cells at the injury site cause nearby capillaries to widen and become more permeable. 1 Chemokines released by various kinds of cells attract more phagocytic cells from the blood to the injury site. 3 Neutrophils and macrophages phagocytose pathogens and cell debris at the site, and the tissue heals. 4 Figure 43.6 Major events in the local inflammatory response

12. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Phagocytic Cells Phagocytes attach to their prey via surface receptors – And engulf them, forming a vacuole that fuses with a lysosome Figure 43.4 Pseudopodia surround microbes. 1 Microbes are engulfed into cell. 2 Vacuole containing microbes forms. 3 Vacuole and lysosome fuse. 4 Toxic compounds and lysosomal enzymes destroy microbes. 5 Microbial debris is released by exocytosis. 6 Microbes MACROPHAGE Vacuole Lysosome containing enzymes

13. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Concept 43.2: In acquired immunity, lymphocytes provide specific defenses against infection Acquired immunity – Is the body’s second major kind of defense – Involves the activity of lymphocytes

14. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Figure 43.5 The human lymphatic system 1 2 3 4 Interstitial fluid bathing the tissues, along with the white blood cells in it, continually enters lymphatic capillaries. Fluid inside the lymphatic capillaries, called lymph, flows through lymphatic vessels throughout the body. Within lymph nodes, microbes and foreign particles present in the circulating lymph encounter macro- phages, dendritic cells, and lymphocytes, which carry out various defensive actions. Lymphatic vessels return lymph to the blood via two large ducts that drain into veins near the shoulders. Adenoid Tonsil Lymph nodes Spleen Peyer’s patches (small intestine) Appendix Lymphatic vessels Lymph node Masses of lymphocytes and macrophages Tissue cells Lymphatic vessel Blood capillary Lymphatic capillary Interstitial fluid

15. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Antigen- binding sites Antibody A Antigen Antibody B Antibody C Epitopes (antigenic determinants) An antigen is any foreign molecule – That is specifically recognized by lymphocytes and elicits a response from them A lymphocyte actually recognizes and binds – To just a small, accessible portion of the antigen called an epitope Figure 43.7

16. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Antigen Recognition by Lymphocytes The vertebrate body is populated by two main types of lymphocytes – B lymphocytes (B cells) and T lymphocytes (T cells) – Which circulate through the blood The plasma membranes of both B cells and T cells – Have about 100,000 antigen receptor that all recognize the same epitope

17. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Clonal Selection of Lymphocytes In a primary immune response – Binding of antigen to a mature lymphocyte induces the lymphocyte’s proliferation and differentiation, a process called clonal selection

18. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Clonal selection of B cells – Generates a clone of short-lived activated effector cells and a clone of long-lived memory cells Figure 43.12 Antigen molecules Antigen receptor B cells that differ in antigen specificity Antibody molecules Clone of memory cells Clone of plasma cells Antigen molecules bind to the antigen receptors of only one of the three B cells shown. The selected B cell proliferates, forming a clone of identical cells bearing receptors for the selecting antigen. Some proliferating cells develop into short-lived plasma cells that secrete antibodies specific for the antigen. Some proliferating cells develop into long-lived memory cells that can respond rapidly upon subsequent exposure to the same antigen.

19. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Primary and Secondary Immune Responses In the secondary immune response, memory cells facilitate a faster, more efficient response Antibody concentration (arbitrary units) 10 4 10 3 10 2 10 1 10 0 0 7 14 21 28 35 424956 Time (days) Figure 43.13 Antibodies to A Antibodies to B Primary response to antigen A produces anti- bodies to A 2 Day 1: First exposure to antigen A 1 Day 28: Second exposure to antigen A; first exposure to antigen B 3 Secondary response to anti- gen A produces antibodies to A; primary response to anti- gen B produces antibodies to B 4

20. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Humoral and cell-mediated immunity defend against different types of threats Acquired immunity includes two branches – The humoral immune response involves the activation and clonal selection of B cells, resulting in the production of secreted antibodies – The cell-mediated immune response involves the activation and clonal selection of cytotoxic T cells

21. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The roles of the major participants in the acquired immune response Figure 43.14 Humoral immune response Cell-mediated immune response First exposure to antigen Intact antigens Antigens engulfed and displayed by dendritic cells Antigens displayed by infected cells Activate Gives rise to B cell Helper T cell Cytotoxic T cell Plasma cells Memory B cells Active and memory helper T cells Memory cytotoxic T cells Active cytotoxic T cells Secrete antibodies that defend against pathogens and toxins in extracellular fluid Defend against infected cells, cancer cells, and transplanted tissues Secreted cytokines activate

22. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Figure 43.10 Overview of lymphocyte development Bone marrow Lymphoid stem cell B cell Thymus T cell Blood, lymph, and lymphoid tissues (lymph nodes, spleen, and others)

23. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Antibody-Mediated Disposal of Antigens The binding of antibodies to antigens – Is also the basis of several antigen disposal mechanisms – Leads to elimination of microbes by phagocytosis and complement-mediated lysis

24. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Antibody-mediated mechanisms of antigen disposal Binding of antibodies to antigens inactivates antigens by Viral neutralization (blocks binding to host) and opsonization (increases phagocytosis) Agglutination of antigen-bearing particles, such as microbes Precipitation of soluble antigens Activation of complement system and pore formation Bacterium Virus Bacteria Soluble antigens Foreign cell Complement proteins MAC Pore Enhances Phagocytosis Leads to Cell lysis Macrophage Figure 43.19

25. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Active and Passive Immunization Active immunity – Develops naturally in response to an infection – Can also develop following immunization, also called vaccination

26. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings In immunization – A nonpathogenic form of a microbe or part of a microbe elicits an immune response to an immunological memory for that microbe

27. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Passive immunity, which provides immediate, short-term protection – Is conferred naturally when IgG crosses the placenta from mother to fetus or when IgA passes from mother to infant in breast milk – Can be conferred artificially by injecting antibodies into a nonimmune person

28. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Concept 43.4: The immune system’s ability to distinguish self from nonself limits tissue transplantation The immune system – Can wage war against cells from other individuals Transplanted tissues – Are usually destroyed by the recipient’s immune system

29. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Recipient-donor combinations – Can be fatal or safe Table 43.1

30. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Figure 43.20 Mast cells, IgE, and the allergic response IgE antibodies produced in response to initial exposure to an allergen bind to receptors or mast cells. 1 On subsequent exposure to the same allergen, IgE molecules attached to a mast cell recog- nize and bind the allergen. 2 Degranulation of the cell, triggered by cross-linking of adjacent IgE molecules, releases histamine and other chemicals, leading to allergy symptoms. 3 1 2 3 Allergen IgE Histamine Granule Mast cell

31. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Autoimmune Diseases In individuals with autoimmune diseases – The immune system loses tolerance for self and turns against certain molecules of the body

32. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Rheumatoid arthritis Is an autoimmune disease that leads to damage and painful inflammation of the cartilage and bone of joints Figure 43.21

33. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Other examples of autoimmune diseases: Systemic lupus erythematosus (lupus) – Antibodies against a wide range of self molecules Multiple sclerosis – Destruction of myelin sheath surrounding nerves Insulin-dependent diabetes – Insulin- producing pancreas cells are targeted

34. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Acquired Immunodeficiency Syndrome (AIDS) Because AIDS arises from the loss of helper T cells – Both humoral and cell-mediated immune responses are impaired People with AIDS – Are highly susceptible to opportunistic infections and cancers that take advantage of an immune system in collapse

35. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The loss of helper T cells – Results from infection by the human immunodeficiency virus (HIV) 1µm Figure 43.22

36. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The spread of HIV – Has become a worldwide problem The best approach for slowing the spread of HIV – Is educating people about the practices that transmit the virus