1. LECTURE PRESENTATIONS For CAMPBELL BIOLOGY, NINTH EDITION Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert B. Jackson © 2011 Pearson Education, Inc. Lectures by Erin Barley Kathleen Fitzpatrick Cellular Respiration and Fermentation Chapter 9

2. Figure 9.1

3. Figure 9.2 Light energy ECOSYSTEM Photosynthesis in chloroplasts Cellular respiration in mitochondria CO 2  H 2 O  O 2 Organic molecules ATP powers most cellular work ATP Heat energy

4. Catabolic Pathways and Production of ATP The breakdown of organic molecules is exergonic Fermentation is a partial degradation of sugars that occurs without O 2 Aerobic respiration consumes organic molecules and O 2 and yields ATP Anaerobic respiration is similar to aerobic respiration but consumes compounds other than O 2 © 2011 Pearson Education, Inc.

5. Cellular respiration includes both aerobic and anaerobic respiration but is often used to refer to aerobic respiration C 6 H 12 O 6 + 6 O 2  6 CO 2 + 6 H 2 O + Energy (ATP + heat) © 2011 Pearson Education, Inc.

6. Redox Reactions: Oxidation and Reduction transfer of electrons during chemical reactions releases energy stored in organic molecules released energy is ultimately used to synthesize ATP © 2011 Pearson Education, Inc.

7. The Principle of Redox Chemical reactions that transfer electrons between reactants are called oxidation- reduction reactions, or redox reactions oxidation - a substance loses electrons, or is oxidized reduction - a substance gains electrons, or is reduced (the amount of positive charge is reduced) © 2011 Pearson Education, Inc.

8. Figure 9.UN01 becomes oxidized (loses electron) becomes reduced (gains electron)

9. Figure 9.UN02 becomes oxidized becomes reduced

10. Redox LEO – loses electrons and is oxidized GER –gains electrons and is reduced Reducing agent – gives away the electrons Oxidizing agent – receives the electrons

11. Oxidation of Organic Fuel Molecules During Cellular Respiration During cellular respiration, the fuel (such as glucose) is oxidized, and O 2 is reduced © 2011 Pearson Education, Inc.

12. Figure 9.UN03 becomes oxidized becomes reduced

13. The Stages of Cellular Respiration: A Preview Harvesting of energy from glucose has three stages –Glycolysis (breaks down glucose into two molecules of pyruvate) –The citric acid cycle (completes the breakdown of glucose) –Oxidative phosphorylation (accounts for most of the ATP synthesis) © 2011 Pearson Education, Inc.

14. Glycolysis 2 Net ATP 2 NADH 2 pyruvate molecules

15. Figure 9.6-1 Electrons carried via NADH Glycolysis Glucose Pyruvate CYTOSOL MITOCHONDRION ATP Substrate-level phosphorylation

16. Figure 9.6-2 Electrons carried via NADH Electrons carried via NADH and FADH 2 Citric acid cycle Pyruvate oxidation Acetyl CoA Glycolysis Glucose Pyruvate CYTOSOL MITOCHONDRION ATP Substrate-level phosphorylation

17. Figure 9.6-3 Electrons carried via NADH Electrons carried via NADH and FADH 2 Citric acid cycle Pyruvate oxidation Acetyl CoA Glycolysis Glucose Pyruvate Oxidative phosphorylation: electron transport and chemiosmosis CYTOSOL MITOCHONDRION ATP Substrate-level phosphorylation Oxidative phosphorylation

18. The process that generates most of the ATP is called oxidative phosphorylation because it is powered by redox reactions © 2011 Pearson Education, Inc.

19. Glycolysis Glucose broken down to 2 pyruvate 9 intermediates (with own enzyme) occur in the process Cell reduces 2 NAD+ to NADH 2 ATP formed by substrate phosphorylatino Pyruvate moved to mitochondria (by active transport)

20. Glycolysis (cont.) Glycolysis occurs in the cytosol Does not require oxygen 4 ATP produced but 2 used to convert glucose to the intermediates.

21. Figure 9.8 Energy Investment Phase Glucose 2 ADP  2 P 4 ADP  4 P Energy Payoff Phase 2 NAD +  4 e   4 H + 2 Pyruvate  2 H 2 O 2 ATP used 4 ATP formed 2 NADH  2 H + Net Glucose 2 Pyruvate  2 H 2 O 2 ATP 2 NADH  2 H + 2 NAD +  4 e   4 H + 4 ATP formed  2 ATP used

22. Figure 9.10 Pyruvate Transport protein CYTOSOL MITOCHONDRION CO 2 Coenzyme A NAD  + H  NADH Acetyl CoA 123

23. Pyruvate Prep for Citric Acid cycle Pyruvate converted to acetyl CoA-----  Carboxyl group (-COO-) removed from pyruvate and given off as CO 2.  Two carbons remaining are oxidized to form acetic acid  NAD+ is reduced to NADH  Coenzyme A joins the two carbons and forms acetyl CoA  1 glucose produces 2 Acetyl CoA

24. Figure 9.11 Pyruvate NAD  NADH + H  Acetyl CoA CO 2 CoA 2 CO 2 ADP + P i FADH 2 FAD ATP 3 NADH 3 NAD  Citric acid cycle + 3 H 

25. Citric Acid Cycle Acetyl CoA enters cycle Joins 4 carbon molecules Redox reactions occur and release CO 2 4 carbon molecule regenerated Occurs twice 1 ATP1 FADH 2 3 NADH

26. Figure 9.12-8 NADH 1 Acetyl CoA Citrate Isocitrate  -Ketoglutarate Succinyl CoA Succinate Fumarate Malate Citric acid cycle NAD  NADH FADH 2 ATP + H  NAD  H2OH2O H2OH2O ADP GTPGDP P i FAD 3245678 CoA-SH CO2CO2 CO2CO2 Oxaloacetate

27. The Pathway of Electron Transport inner membrane (cristae) of the mitochondrion exist in multiprotein complexes 3 proteins transport H+ across the membrane A H+ gradient results An ATP synthase is built into the membrane The synthase attach phosphates to ADP The oxygen in the matrix accepts the electrons from the chain and bonds two H ions to form © 2011 Pearson Education, Inc.

28. Electrons are transferred from NADH or FADH 2 to the electron transport chain © 2011 Pearson Education, Inc.

29. Figure 9.13 NADH FADH 2 2 H  + 1 / 2 O 2 2 e  H2OH2O NAD  Multiprotein complexes (originally from NADH or FADH 2 ) I II III IV 50 40 30 20 10 0 Free energy (G) relative to O 2 (kcal/mol) FMN Fe  S FAD Q Cyt b Cyt c 1 Cyt c Cyt a Cyt a 3 Fe  S

30. Figure 9.14 INTERMEMBRANE SPACE Rotor Stator HH Internal rod Catalytic knob ADP + P i ATP MITOCHONDRIAL MATRIX

31. Figure 9.15 Protein complex of electron carriers (carrying electrons from food) Electron transport chain Oxidative phosphorylation Chemiosmosis ATP synth- ase I II III IV Q Cyt c FAD FADH 2 NADH ADP  P i NAD  HH 2 H  + 1 / 2 O 2 HH HH HH 21 HH H2OH2O ATP

32. The energy stored in a H + gradient across a membrane couples the redox reactions of the electron transport chain to ATP synthesis The H + gradient is referred to as a proton- motive force, emphasizing its capacity to do work © 2011 Pearson Education, Inc.

33. An Accounting of ATP Production by Cellular Respiration During cellular respiration, most energy flows in this sequence: glucose  NADH  electron transport chain  proton-motive force  ATP About 34% of the energy in a glucose molecule is transferred to ATP during cellular respiration, making about 32 ATP There are several reasons why the number of ATP is not known exactly © 2011 Pearson Education, Inc.

34. Figure 9.16 Electron shuttles span membrane MITOCHONDRION 2 NADH 6 NADH 2 FADH 2 or  2 ATP  about 26 or 28 ATP Glycolysis Glucose 2 Pyruvate Pyruvate oxidation 2 Acetyl CoA Citric acid cycle Oxidative phosphorylation: electron transport and chemiosmosis CYTOSOL Maximum per glucose: About 30 or 32 ATP

35. Concept 9.5: Fermentation and anaerobic respiration enable cells to produce ATP without the use of oxygen Most cellular respiration requires O 2 to produce ATP Without O 2, the electron transport chain will cease to operate In that case, glycolysis couples with fermentation or anaerobic respiration to produce ATP © 2011 Pearson Education, Inc.

36. Types of Fermentation Fermentation consists of glycolysis plus reactions that regenerate NAD +, which can be reused by glycolysis Two common types are alcohol fermentation and lactic acid fermentation © 2011 Pearson Education, Inc.

37. In alcohol fermentation, pyruvate is converted to ethanol in two steps, with the first releasing CO 2 Alcohol fermentation by yeast is used in brewing, winemaking, and baking © 2011 Pearson Education, Inc.

38. Figure 9.17 2 ADP 2 ATP Glucose Glycolysis 2 Pyruvate 2 CO 2 2  2 NADH 2 Ethanol 2 Acetaldehyde (a) Alcohol fermentation (b) Lactic acid fermentation 2 Lactate 2 Pyruvate 2 NADH Glucose Glycolysis 2 ATP 2 ADP  2 P i NAD 2 H   2 P i 2 NAD    2 H 

39. In lactic acid fermentation, pyruvate is reduced to NADH, forming lactate as an end product, with no release of CO 2 Lactic acid fermentation by some fungi and bacteria is used to make cheese and yogurt Human muscle cells use lactic acid fermentation to generate ATP when O 2 is scarce © 2011 Pearson Education, Inc.

40. (b) Lactic acid fermentation 2 Lactate 2 Pyruvate 2 NADH Glucose Glycolysis 2 ADP  2 P i 2 ATP 2 NAD   2 H  Figure 9.17b

41. Comparing Fermentation with Anaerobic and Aerobic Respiration All use glycolysis In all three, NAD + is the oxidizing agent that The processes have different final electron acceptors: an organic molecule (such as pyruvate or acetaldehyde) in fermentation and O 2 in cellular respiration Cellular respiration produces 32 ATP per glucose molecule; fermentation produces 2 ATP per glucose molecule © 2011 Pearson Education, Inc.

42. Obligate anaerobes carry out fermentation or anaerobic respiration and cannot survive in the presence of O 2 Yeast and many bacteria are facultative anaerobes, meaning that they can survive using either fermentation or cellular respiration In a facultative anaerobe, pyruvate is a fork in the metabolic road that leads to two alternative catabolic routes © 2011 Pearson Education, Inc.

43. The Evolutionary Significance of Glycolysis Ancient prokaryotes are thought to have used glycolysis long before there was oxygen in the atmosphere Very little O 2 was available in the atmosphere until about 2.7 billion years ago, so early prokaryotes likely used only glycolysis to generate ATP Glycolysis is a very ancient process © 2011 Pearson Education, Inc.

44. Concept 9.6: Glycolysis and the citric acid cycle connect to many other metabolic pathways Gycolysis and the citric acid cycle are major intersections to various catabolic and anabolic pathways © 2011 Pearson Education, Inc.

45. The Versatility of Catabolism Catabolic pathways funnel electrons from many kinds of organic molecules into cellular respiration Glycolysis accepts a wide range of carbohydrates Proteins must be digested to amino acids; amino groups can feed glycolysis or the citric acid cycle © 2011 Pearson Education, Inc.

46. Fats are digested to glycerol (used in glycolysis) and fatty acids (used in generating acetyl CoA) Fatty acids are broken down by beta oxidation and yield acetyl CoA An oxidized gram of fat produces more than twice as much ATP as an oxidized gram of carbohydrate © 2011 Pearson Education, Inc.

47. Biosynthesis (Anabolic Pathways) The body uses small molecules to build other substances These small molecules may come directly from food, from glycolysis, or from the citric acid cycle © 2011 Pearson Education, Inc.

48. Regulation of Cellular Respiration via Feedback Mechanisms Feedback inhibition is the most common mechanism for control If ATP concentration begins to drop, respiration speeds up; when there is plenty of ATP, respiration slows down Control of catabolism is based mainly on regulating the activity of enzymes at strategic points in the catabolic pathway © 2011 Pearson Education, Inc.