1. CONFIDENTIAL Applications for Field Service Engineers

2. CONFIDENTIAL HPLC Applications Training

3. CONFIDENTIAL HPLC Applications Training

4. CONFIDENTIAL HPLC Applications Training

5. CONFIDENTIAL HPLC Applications Training

6. CONFIDENTIAL HPLC Applications Training

7. CONFIDENTIAL HPLC Applications Training MOBILE PHASE CONSIDERATIONS  Flammability  Toxicity  Miscibility  Viscosity  Dissolved Salts  %Organic Solvent  pH (Acid/Alkali)  Carbon Dioxide  Corrosion

8. CONFIDENTIAL HPLC Applications Training Some Flammable Solvents Commonly Used  Acetonitrile  Methanol  Hexane, iso-Hexanes, Heptane, iso-Octane  Tetrahydrofuran  Toluene  Acetone  Ethyl Acetate  Isopropanol

9. CONFIDENTIAL HPLC Applications Training Some Toxic Solvents Commonly Used  Acetonitrile  Methanol  Hexane  Tetrahydrofuran  Toluene  Dichloromethane (Methylene Chloride)  Chloroform  Dimethyl Sulphoxide

10. CONFIDENTIAL HPLC Applications Training BURDICK & JACKSON UV Cutoff (nm) BURDICK & JACKSON UV Cutoff (nm) Acetonitrile UV190 Ethylene Dichloride228 Water190 Dichloromethane233 Hexane UV195 Chloroform245 Cyclohexane200 Ethyl Acetate256 Heptane200 Dimethyl Acetamide268 Isopropyl Alcohol205 DMF268 Dimethyl Sulfoxide268 Methanol205 Toluene284 Ethyl Alcohol210 Trifluoroacetic Acid210 1,2,4-TCB308 Tetrahydrofuran UV212 1,4-Dioxane215 Acetone330 Ethyl Ether215 Methyl Isoamyl Ketone330 Iso-Octane215 Methyl Isobutyl Ketone334

11. CONFIDENTIAL HPLC Applications Training HPLC SOLVENTS - UV CUTOFF Burdick & Jackson solvents are arranged in order of increasing UV cutoff, the wavelength at which the solvent absorbance in a 1 cm path length cell is equal to 1 AU (absorbance unit) using water in the reference cell. UV Cutoff (nm) Acetonitrile UV190 Pentane 190 Water 190 Hexane UV 195 Cyclopentane198 Cyclohexane200 Heptane200 Isopropyl Alcohol205 Methanol205 Ethyl Alcohol210 2-Methoxyethanol210 Methyl t-Butyl Ether210 n-Propyl Alcohol210 Trifluoroacetic Acid210 Tetrahydrofuran UV212 n-Butyl Alcohol215 1,4-Dioxane215 Ethyl Ether215

12. CONFIDENTIAL HPLC Applications Training

13. CONFIDENTIAL HPLC Applications Training

14. CONFIDENTIAL HPLC Applications Training

15. CONFIDENTIAL HPLC Applications Training

16. CONFIDENTIAL HPLC Applications Training

17. CONFIDENTIAL HPLC Applications Training

18. CONFIDENTIAL HPLC Applications Training

19. CONFIDENTIAL HPLC Applications Training

20. CONFIDENTIAL HPLC Applications Training

21. CONFIDENTIAL HPLC Applications Training HPLC Columns  Silica particles (1.5~100μm)  Polymer particles (3~50µm)

22. CONFIDENTIAL HPLC Applications Training

23. CONFIDENTIAL HPLC Applications Training

24. CONFIDENTIAL HPLC Applications Training SAMPLE PREPARATION  Filtration – Membrane Filters 0.2µm, 0.45µm (Cellulose Acetate, PVDF)  Centrifugation – Economical, less contamination, multiple processing

25. CONFIDENTIAL HPLC Applications Training PS335 Photodiode Array Detector

26. CONFIDENTIAL HPLC Applications Training PS335 Photodiode Array Detector

27. CONFIDENTIAL HPLC Applications Training PS335 Photodiode Array Detector

28. CONFIDENTIAL HPLC Applications Training PS335 Photodiode Array Detector

29. CONFIDENTIAL HPLC Applications Training PS335 Photodiode Array Detector

30. CONFIDENTIAL Applications for Field Service Engineers