1. Proteins, amino acids and exercise

2. Proteins and amino acids Proteins Proteins the most important biological compounds needed for life the most important biological compounds needed for life act as the structural materials in humans act as the structural materials in humans Enzymes are proteins that catalyze the body's chemical reactions. Enzymes are proteins that catalyze the body's chemical reactions. make up muscles that aid in movement. make up muscles that aid in movement. Amino acids Amino acids Proteins are made up of chains of amino acids. Proteins are made up of chains of amino acids. an amine group (-NH 2 ) bonded to a carbon atom that is bonded to a carboxylic acid group (-COOH) an amine group (-NH 2 ) bonded to a carbon atom that is bonded to a carboxylic acid group (-COOH)

4. Amino acids can bond together by peptide bond

5. Amino acids (AA) 20 amino acids in the nature 20 amino acids in the nature Classification of amino acids Classification of amino acids Essential AA - methionine, leucine, isoleucine, lysine, phenylalanine, threonine, tryptophan, and valine Essential AA - methionine, leucine, isoleucine, lysine, phenylalanine, threonine, tryptophan, and valine Conditionally essential AA - histidine, arginine (required for infants) Conditionally essential AA - histidine, arginine (required for infants) Nonesential AA – glycine, aspartic acid, asparagine, proline, glutamine, glutamic acid, cysteine, tyrosine, serine, alanine, hydroxyproline Nonesential AA – glycine, aspartic acid, asparagine, proline, glutamine, glutamic acid, cysteine, tyrosine, serine, alanine, hydroxyproline

6. Digestion and absorption of protein Digestion Digestion The stomach The stomach Hydrochloric acid, pepsine Hydrochloric acid, pepsine Proteins => polypetides, amino acids Proteins => polypetides, amino acids The small intestine The small intestine pancreatic and intestinal proteases pancreatic and intestinal proteases Polypeptide => oligopeptides, tripeptides, dipeptides, amino acids Polypeptide => oligopeptides, tripeptides, dipeptides, amino acids Peptidase Peptidase Tripeptides and dipeptides => amino acids Tripeptides and dipeptides => amino acids Absorption Absorption Specific carriers transport AA Specific carriers transport AA

7. Roles of proteins in the body As a building material As a building material As enzymes As enzymes As hormones As hormones As regulator of fluid balance As regulator of fluid balance As acid-base regulators As acid-base regulators As transporters As transporters As antibodies As antibodies As a source of energy and glucose As a source of energy and glucose

8. Protein metabolism Protein turnover and the amino acid pool Protein turnover and the amino acid pool proteins are being made and broken down proteins are being made and broken down Nitrogen balance Nitrogen balance Positive – growing infants, childrens, pregnant women Positive – growing infants, childrens, pregnant women Negative – people who are starving or suffering severe stress Negative – people who are starving or suffering severe stress Using AA to make proteins, nonessential AA Using AA to make proteins, nonessential AA Using AA to make other compounds Using AA to make other compounds Tyrosine → neurotransmitters - norepinephrine, pigment melanin, hormon thyroxin, precursor for the vitamin niacin Tyrosine → neurotransmitters - norepinephrine, pigment melanin, hormon thyroxin, precursor for the vitamin niacin Using AA for energy Using AA for energy Glucose, fatty acid is limited => amino acids are source of energy Glucose, fatty acid is limited => amino acids are source of energy Deamination AA Deamination AA Broken down AA (source of energy) Broken down AA (source of energy) => stripped of their nitrogen-containing amino groups (NH 2 ) => stripped of their nitrogen-containing amino groups (NH 2 ) ammonia => urea ammonia => urea Using AA to make fat Using AA to make fat If a person eat a lot of protein => convert to fat and store If a person eat a lot of protein => convert to fat and store

9. Proteins in food and their quality Source of protein Source of protein Animal source – meat, fish, milk and dairy products, egg Animal source – meat, fish, milk and dairy products, egg Plant source – lentils, legumes, nuts, whole grains, vegetables Plant source – lentils, legumes, nuts, whole grains, vegetables Limiting AA Limiting AA e.g. lysine in grains, methionine in legumes e.g. lysine in grains, methionine in legumes Complete protein Complete protein = animal protein – contains all esential AA Biological value (BV) Biological value (BV) A measure of protein quality A measure of protein quality The amount of protein nitrogen that is retained from a given amount of protein nitrogen absorbed The amount of protein nitrogen that is retained from a given amount of protein nitrogen absorbed BV egg 100, meat 92 – 92, fish 94-96, legumes 75-80, grains 70 BV egg 100, meat 92 – 92, fish 94-96, legumes 75-80, grains 70

10. RDA of protein 12 – 15 % energy 12 – 15 % energy Diet 2000 kcal = 300 kcal from protein = 75 g Diet 2000 kcal = 300 kcal from protein = 75 g Age Protein RDA (g/kg) 11- 14 1,0 15 - 18 0,8 – 0,9 Adult0,8 Endurance athlete 1,2 -1,4 Strength athlete 1,4 – 1,8 Children athlete 1,5

11. Approximate protein content of various foods Food Protein (g) Beef 3 oz 28 Pork 3 oz 28 Cod 3 oz 21 Oysters 3 oz 17 Milk 1 c 8 Cheddar cheese1 oz 7 Egg 1 large 6 Peanut butter (1 tbs) 8 Po1 medtato 1 3 Bread 1 slice 2 Banana1 Carrots 2 c 1 Apple 1 2 Sugar, oil 0

12. Metabolism of amino acids and protein during exercise Amino acid x not source of energy Amino acid x not source of energy Hormones influence muscle protein turnover Hormones influence muscle protein turnover Influence synthesis, breakdown or both Influence synthesis, breakdown or both Anabolic - synthesis Anabolic - synthesis Catabolic – breakdown Catabolic – breakdown Single exercise bout modify the amount of circulating levels of hormones Single exercise bout modify the amount of circulating levels of hormones Growth hormone, tyrosin ↑ Growth hormone, tyrosin ↑

13. Hormones influence muscle protein turnover Insulin Insulin Release is stimulated by elevated blod glucose and less potently by elevation of AA Release is stimulated by elevated blod glucose and less potently by elevation of AA Promote uptake of AA to cells (skeletal muscle) Promote uptake of AA to cells (skeletal muscle) Promote a synthesis of protein, limiting break down Promote a synthesis of protein, limiting break down During exercise is release of insulin blunted (greater glycogen and fat breakdown) During exercise is release of insulin blunted (greater glycogen and fat breakdown) Endurance exercise - more AA available for gluconeogenesis Endurance exercise - more AA available for gluconeogenesis

14. Hormones influence muscle protein turnover Cortisol Cortisol Released by the adrenal gland during physiological or nutritional stress Released by the adrenal gland during physiological or nutritional stress Stress hormone Stress hormone Increase energy nutrient availaility – breakdown protein Increase energy nutrient availaility – breakdown protein Vary in level during exercise (intensity, duration) Vary in level during exercise (intensity, duration) Growth hormone (GH) Growth hormone (GH) Increase during exercise, remain elevated for some time afterward Increase during exercise, remain elevated for some time afterward Increase level of AA after protein rich meal => ↑ GH Increase level of AA after protein rich meal => ↑ GH ↑ GH => ↑ AA uptake to muscle cells, protein synthesis ↑ GH => ↑ AA uptake to muscle cells, protein synthesis

15. Hormones influence muscle protein turnover Insuline-like growth factor 1 (IGF-1) Insuline-like growth factor 1 (IGF-1) Anabolic efect of GH is mediated with IGF-1 Anabolic efect of GH is mediated with IGF-1 If IGF-1 is ↓ and GH is ↑ = inhibited protein synthesis If IGF-1 is ↓ and GH is ↑ = inhibited protein synthesis Testosterone Testosterone Promoter of protein synthesis in muscle Promoter of protein synthesis in muscle Enhance utilization of AA for protein synthesis Enhance utilization of AA for protein synthesis Level of testosterone ↑ = resistance and endurance training Level of testosterone ↑ = resistance and endurance training

16. Resistance exercise (RE) Protein synthesis Protein synthesis unchanged or reduced during RE unchanged or reduced during RE Increase for several hours after RE Increase for several hours after RE Protein breakdown Protein breakdown Not occur during RE Not occur during RE May increase after RE May increase after RE AA oxidation AA oxidation Not enhanced during RE Not enhanced during RE Consumption of a carbohydrate and protein meal after RE Consumption of a carbohydrate and protein meal after RE => minimize protein breakdown => maximize protein synthesis => maximize protein synthesis

17. Endurance exercise (EE) Protein synthesis Protein synthesis unchanged or reduced during EE unchanged or reduced during EE Protein breakdown Protein breakdown can occur during higher intensity, longer duration EE can occur during higher intensity, longer duration EE AA oxidation AA oxidation Can occur during prolonged moderate to higher intensity submaximal EE Can occur during prolonged moderate to higher intensity submaximal EE Consumption of carbohydrate and protein meal after EE Consumption of carbohydrate and protein meal after EE => minimize protein breakdown => maximize protein synthesis => maximize protein synthesis

18. Protein intake of athletes Endurance athlete 1,2 - 1,4 g/kg Endurance athlete 1,2 - 1,4 g/kg Strength athlete 1,4 – 1,8 g/kg Strength athlete 1,4 – 1,8 g/kg Timing and composition of meal - postexercise Timing and composition of meal - postexercise Carbohydrates – fuel and increase the uptake of AA Carbohydrates – fuel and increase the uptake of AA AA – allows for an influx into muscle cells, protein synthesis AA – allows for an influx into muscle cells, protein synthesis After training After training 0,5 g protein per kg + 1,5 g carbohydrates per kg 0,5 g protein per kg + 1,5 g carbohydrates per kg Weight training - at lest 135 g of carb. and 45 g of protein for a 90 kg weight trainer Weight training - at lest 135 g of carb. and 45 g of protein for a 90 kg weight trainer Endurance training – at least 105 g of carb. and 35 g of protein for a 73 kg endurance athlete Endurance training – at least 105 g of carb. and 35 g of protein for a 73 kg endurance athlete = 560 – 720 kcal (1/5 – 1/6 daily energy needs) After 3-4hour eat again After 3-4hour eat again => Maximise positive efect of exercise on muscle protein turnover

19. Protein intake of athletes Timing and composition of meal – during exercise Timing and composition of meal – during exercise Carbohydrate consumption (e.g. Sport drinks) – support better recovery and adaptation Carbohydrate consumption (e.g. Sport drinks) – support better recovery and adaptation Number of meals Number of meals Numerous smaller meals over the waking hours Numerous smaller meals over the waking hours = more consistent influence on insulin level = minimize catabolic periods between meals = more consistent availability essential AA = more desirable influence on protein synthesis 5 – 6 meal during a day 5 – 6 meal during a day After exercise approximately 40 g protein After exercise approximately 40 g protein Each other meal 15 – 20 g of protein Each other meal 15 – 20 g of protein