1. Abdominal and Thoracic Effusions Clinical Pathology

2. Abdominal/thoracic fluids Abdominal and thoracic organs are bathed in and lubricated by a small amount of fluid Fluid increases when the amount entering the cavity is more than is removed fro it An increased amount of fluid in the abdominal/thoracic cavity is not a disease in itself, but rather an indication of a pathologic process in the fluid production and/or removal system.

4. Abdominal/Thoracic Effusion Fluid analysis and cytology is quick, easy and inexpensive, and relatively safe. May obtain useful information for diagnosis, prognosis, and treatment.

6. Abdominocentesis Collection Aseptic prep of the skin Usually done with animal standing Use a sterile needle or cannula Tap the ventral midline of the abdomen, 1-2 cm caudal to the umbilicus Collect fluid and place into an EDTA and red top tube

7. Indications of a peritoneal tap Ascites: due to cardiac or liver disease or neoplasia, etc. Peritonitis: Ruptured bowel, ruptured bladder. In horses-colic

9. Thoracentesis Pleural effusion may be bilateral or unilateral. Radiographs help determine the extent and location. Usually place in sternal recumbency. Aseptic prep. Sterile needle, catheter inserted next to cranial surface of the rib to prevent risk of penetrating the vessel on the caudal border of the rib.

10. Indications for Thoracentesis Hemorrhage Inflammation (FIP) Neoplasia Ruptured thoracic lymphatic duct (chylothorax) Place in EDTA and Red top tube

11. Characteristics of Effusions Transparency/turbidity Color Protein concentration Specific gravity Cells: counts, types, and morphology Fluid should be odorless- if an abdominal tap yields a malodorous fluid- may indicate a ruptured bowel

13. Color/turbidity of Effusions Influenced by protein concentrations and cell numbers. Normal peritoneal/pleural fluid is colorless and transparent to slightly turbid. FIP may cause an amber, turbid, thick effusion (straw-yellow color)

15. Total Protein and Specific Gravity Centrifuge sample at low speed (1500 rpm for 5 min) TP can be measured on refractometer Normal is <2.5 g/dl Specific gravity is measured on refractometer as well Normal is <1.018

16. Slide preparation Centrifuge fluid at low speed Pour off supernatant, leave about 0.5 ml at the bottom of tube Resuspend by gentle agitation Place a drop on slide Routine blood smear type technique Squash prep Air dry Stain with diff quick

17. Cell counts on Effusions Total nucleated cell counts Unopette procedure Automated Normal peritoneal/pleural fluid has <10,000 nucleated cells/ul Estimated cell counts can be made on a blood smear

18. Types of cells found in effusions Neutrophils Mesothelial/macrophage type cells Lymphocytes Eosinophils Mast cells Neoplastic cells

19. Classifications of Effusions Transudates Modified transudates Exudates

20. Transudates Clear, colorless effusion <2.5 g/dl protein (low protein) Low total nucleated cell count Non-degenerative neutrophils and mesothelial cells Specific gravity < 1.013

22. Causes of Transudates Hypoalbunemia: due to renal glomerular disease, hepatic insufficiency, and protein-losing enteropathy. Ruptured bladder Rarely from blockage of lymph from lymphatic vessel in the intestines

23. Modified Transudates Vary in color- amber to white to red Frequently slightly turbid to turbid High protein concentration (2.5-7.5 g/dl) Moderate cellularity: 1000-7000 cells/ul Occur as a result of fluid leakage from lymphatics carrying high protein lymph or blood vessels.

24. Modified transudate causes Lease specific, variety of disorders Cardiovascular disease (right sided heart failure) Neoplastic disease FIP Chylothorax Hemorrhage Hepatic disease- hypoalbunemia and hypertension

25. Exudates (infections) Color varies- amber to white to red Turbid to cloudy High protein > 3.0 g/dl High total nucleated cell count (>7000 cell/ul) Neutrophils are the predominant cell type.

26. Exudates continued Septic exudates: Degenerative neutrophils and intracellular/extracellular bacteria present Ex: GI perforation, systemic sepsis, pneumonia Non-septic exudates: non-degenerative neutrophils, small lymphocytes, and/or neoplastic cells. Ex: Pancreatitis, neoplasia, uroperitoneum

27. Chylous Effusions Contains chylomicron-rich fluid that is present in lymphatics that drain the intestinal tract and pass through the thoracic duct. Chylomicrons are triglyceride-rich lipoproteins absorbed from the intestines after the ingestion of food containing lipids. Chyle normally drains from the thoracic duct into the venous system. Effusion forms when there is an obstruction of the lymphatic flow resulting in increases pressure within the lymphatics and dilation of the thoracic duct. Other causes included: cardiovascular disease or trauma Lymphocytes are predominant type