1. The Patient: Figaro

2. What type of macromolecule is an antibody? What shape is a typical antibody? Protein

3. What is an antigen? How many antigens can the antibody below bind to? Foreign Molecule

5. Figaro ActivityPossible Antigens

6. Brain storm ways to test for possible allergies.

7. Label 3 Petri Plates with initials, date, period, LB Agar, and TRIAL 1, 2 or 3 (depending on the plate).

8. Cut the bottom off a large transfer pipet.

9. Apply a slight suction by compressing the bulb of the pipet between your fingers.

10. Use the transfer pipet to poke holes in the agar. Make 4 holes around the edge of the agar.

11. Make 1 hole in the center of the agar. 1 2 3 4 C

12. In your notebook, draw a Petri plate and label which antigen goes into each outside hole. Allergen 1: ?Allergen 3: ? Allergen 2: ? Allergen 4: ? 1 2 3 4

13. On your Petri plate drawing in your notebook, label the center hole as Figaro’s Blood Serum. Figaro’s Blood Serum: Antibodies C

14. Use a sterile 1 mL pipet to add antigen/ antibody solution to each hole in the agar.

15. Add enough antigen serum so the solution is at the top of the agar hole.

16. Use a different sterile 1 mL pipet for each antigen solution.

17. Fill the center hole with Figaro’s Blood Serum (antibody solution).

18. Repeat these steps for all 3 trials/ Petri plates. Cover all 3 Petri plates and leave undisturbed for 24 hours.

19. Collect Petri plates. Draw Petri plate results in your notebook. Write down observations Form a data table in Excel. Create numeric scale to convert observations to data. Collect data from each trial. Average the data from trials.

20. A precipitin line will appear between one or more of the allergens and the blood serum. Precipitin Lines

21. Record results of each of the 3 trials using a numeric scale –5 = strong precipitation; 0 = no precipitation Calculate average results for each antigen

22. The brighter precipitin lines, the stronger the allergy. Weak Allergy Strong Allergy

23. This lab is an example of an Ouchterlony test. When antibodies diffuse in agar and bind with antigens, it causes agglutination, or a white precipitin line. Agglutination = Precipitin Lines

24. Make an individual data table in Google documents. On the Smart Board, add your group’s data to the table and make a class average data table on your Google spreadsheet below your individual data table.

25. Post your individual data table, class average data table, and your REE PE PA three- paragraph conclusion on a new page for this lab on your Google Site. Results - Go back to the purposes of the lab. Evidence - Numerical evidence. Explanation - Go back to your hypothesis. Possible Errors –2 examples of errors –Avoid future errors Practical Applications –Suggestions to patient/family. –Further testing –Value of this lab/test