1. Dr.Hao Wang & Silogini Thanarajah The role of motility and nutrients in a bacterial colony formation and competition

2. Outlines Definitions Introduction Model for simplified case (one bacterial strain competition) Mathematical Analysis Theorems Competition of two bacterial strains in a petri dish model. Simulations in 1-D, 2-D space Conclusion Model for Liquid case

3. Definitions Motile: Moving or having power to move spontaneously. Immotile: Not moving or lacking the ability to move. Agar: A dried hydrophilic, colloidal substance extracted from various species of red algae; used in solid culture media for bacteria and other microorganisms.

5. Introduction In most natural environments, bacteria fight with neighbors for space and nutrients. Most are harmless, some are beneficial and a few become a threat to our health when they grow and reproduce. Many but not all bacteria exhibit motility, i.e. self-propelled motion, under appropriate circumstances. Motility is an important part in the colonization of plant roots by bacteria. Also, colony formation could help clarify factors influencing biofilm formation and illuminate how groups control the fitness of bacteria. Naturereviewsamicrobiology

6. www.nature.com,scienceblogs.com,pasteur.fr

7. Bacteria display kinds of colony patterns according to the substrate softness and nutrients concentration. Previous studies showed four different colony shapes and recognized a morphological diagram by dividing into four regions like diffusion-limited aggregation-like, eden-like, concentric-ring and fluid spreading. Pnas.org

8. Purpose of this paper is to use bacteria as model organism to study competition and determine which strain will “win” in competition with other strain when the two are mixed in a petri dish. We plug these biological characteristics into simulation programs and observe the outcomes.

9. Agar method vs Liquid method (Bruce Levin’s group experiment) Observation from experiments results: For agar case, motile strain dominates the community. For liquid case, immotile strain dominates the community. Ratio of the 2 strains immotile/motile T00.9103 T240.1714 Ratio of the 2 strains immotile/motile T00.9057 T243.3218

10. Bacterial competition in a petri dish model B 1 -motile strain B 2 -immotile strain

11. Bacteria-substrate model without nutrient diffusion

18. Theorems

20. we placed motile and immotile bacterial strains in the middle of the petri dish and observed the pattern formation. Simulations for 1-D space

21. Motile strain-Agar

22. Immotile strain-Agar

23. Motile vs Immotile

24. Resource-Agar

25. Motile strain and immotile strain total population over the space

26. Simulations for 2-D space We placed motile strain in the middle and the Immotile strain little far from the middle of the petri dish and observed the pattern formation after 1hr, 5hrs, 8hrs and 15hrs.

27. Observarion at t=1: Motile and immotile strains are start to grow on the same position, we placed. Some of the nutrients consume by bacterial strains on the same position.

28. Observation at t=5: Motile strain move and grows around the middle of the petri dish and immotile strain grows on the same position, like narrow. Nutrients consume around the middle of the petri dish.

29. Observation at t=8: Motile strain move fast and grows to over lab immotile strain and immotile strain face for the competition with motile strain for nutrients. More and more nutrients used by bacterial strains surrounding the middle of the petri dish.

30. Observation at t=15: Motile strain grows everywhere even over immotile strain and immotile strain don’t have enough nutrients to eat and survive. Almost all nutrients are used but some are still there.

31. Depends on the initial conditions we will get different pattern formation.

32. Conclusion Bacteria always go extinct due to lack of nutrient after a long time while some nutrient will always be remaining. If we incorporate a nutrient input as chemostat-type models, then the bacterial community can be sustained (“closed”->”open”). From computer stimulations (1-D case): If we put motile and immotile bacterial strains in the middle of the petri dish: initially both grow on the same position after that motile strain move fast and grow on the boundary but the immotile strain grow fast on the middle and finally both will die out. In this case motile strain is dominant. It is consistent to Bruce Levin’s group agar case. For liquid case we have to choose different nutrient equation (Liquid is moving everywhere).

33. From 2-D case: If we put motile strain in the middle and the immotile strain little far from the middle of the petri dish: initially both strains grow on the same position as we placed; later, they overlap in some place, then they compete for nutrients such that a some strange patterns occur; after a long time, motile strain passes over immotile one and thus moves fast and grows everywhere and dominate the bacterial community; Finally (not shown in 2-D simulations), all bacteria go extinct due to “closed” system (no nutrient input).

34. Model for Liquid case

35. Motile-Liquid

36. Immotile-Liquid

37. Resource-Liquid

38. Motile strain and immotile strain total population over the space

39. Comparison to agar case Motile strain move fast and grow everywhere. Immotile strain grow with higher density than motile strain. In liquid case immotile strain is dominant.

40. Conclusion In agar case motile strain is dominant while liquid case immotile strain (liquid is moving everywhere but not agar). Consistent to experiment results of Bruce Levin’s group

41. Thank you!