1. Ex. 7: Aseptic Technique – Inoculating Different Types of Media Key concepts: 1.Different types of bacterial cultures: broth, agar slant, and agar deep. 2.Aseptic transfer of bacteria from one type of culture medium to another. 3.Appropriate use of inoculating loops and needles.

2. Four Basic Forms of Bacterial Culture Media Agar plate Broth Agar Deep Agar Slant

3. Always use sterile instruments to transfer bacteria! Loop and Needle

4. Flame loop before AND after using it

5. Other Sterile Transfer Instruments

6. “Little Finger Technique”

7. NA Broth

8. NA Slant Cultures

9. Agar Deeps: What is happening?

10. Use the Inoculating Needle to inoculate a deep