1. LXS RI PANEL: LARGEST EXTANT RI SERIES 78 STRAINS BETH BENNETT T.E. JOHNSON UNIV OF COLORADO BOULDER, CO

2. DERIVATION OF ILS & ISS 8 WAY CROSS: A, AKR, BALB, C3H, C57, DBA, ISBI, RIII HETEROGENOUS STOCK (HS) LS & SS ILS & ISS Select for differential ethanol sensitivity for 25 generations Inbreed 20 generations

3. RI CONSTRUCTION

4. BODY WT IN FEMALES -Female weight chosen as mapping character -Not selected in progenitor strains -Possible interaction with fertility, lifespan -Easily measured test phenotype for LXS RI -Measured at 30 day intervals in generation 13 -94% inbred, 89% loci fixed -Heritability estimated -QTLs mapped using MapManagerQT

5. Genotyping R.Williams and L. Lu -Begun at N 18 -All Parents within Strain Genotyped -Expect 96% of Loci Fixed -361 Polymorphic Markers Identified -Approximate Marker Density of 4.5 cM

6. 30 DAY WEIGHTS

7. WT DISTRIBUTION AT 60, 90, 120, 150 DAYS

8. Heritability (h 2 ), Genetic correlation (r G ), and Phenotypic correlation ( r P ) 30 Day 60 Day90 Day120 Day150 Day 30 Day. 49.64.53.57.56 60 Day.57. 62.79.83.80 90 Day.49.88.66.86.91 120 Day.53.83.90. 64.95 150 Day.53.84.89.92.72

9. FEMALE BODY WEIGHT IN LXS RIs AT 30 DAY INTERVALS WT IN GRAMS

10. GROWTH RATE AT 30 DAY INTERVALS

11. h 2 for Growth Rate Interval (Days) Mean Growth Rate + SE N strains h 2 (95% CI) 30-601.20 + 0.00497.48 (.39-.56) 60-900.49 + 0.00386.42 (.32-.50) 90-1200.37 + 0.00375.25 (.15-.35) 120-1500.25 + 0.00263.22 (.09-.33)

12. QTL MAPPING FOR BODY WT AT 30 DAYS

13. QTL MAPPING FOR BODY WT AT 60 DAYS LOD=3.4

14. QTL MAPPING FOR BODY WT AT 90, 120 & 150 DAYS LOD=3.2

15. PHENOTYPES BEING ASSESSED IN LXS RI -ETHANOL STUDY: -Ethanol sensitivity & blood ethanol at awakening -Ethanol tolerance -Low dose ethanol activation AGING STUDY: -Lifespan -Dietary restriction responses -Torpor, Food processing efficiency, Hair growth rate