1. Hugh B. Fackrell fackrel@uwindsor.ca Filename: Bunyavirus.ppt
Bunyaviridae
Hugh B. Fackrell
Filename: Bunyavirus.ppt

2. Bunyaviridae Structure Classification Multiplication
Clinical manifestations
Epidemiology
Diagnosis
Control
Baron’s Web Site

3. Structure Spherical enveloped particles virus encoded transcriptasenm
virus encoded transcriptase
2 external glycoproteins
multipartite - 3 segments of SS RNA
antisense
- SS RNA
C=122

4. Classification Genera Bunyaviruses Phlebovirus Nairovirus Uukuvirus
Hantavirus
Immunologically
35 serogroups
300 types & subtypes

5. BunyaViruses Phlebovirus Nairovirus Uukuvirus Hantavirus Bunyaviruses
ARthropod BOorne viruses
Phlebovirus
Nairovirus
Uukuvirus
Hantavirus
ROdent BOrne Viruses
Bunyaviruses

6. Bunyavirus Replication

7. Bunyavirus Replication
- ss RNA
Virus RNA transcriptase
+ mRNA
+ ssRNA
Cytoplasm
Bunyavirus Replication

8. Genetic Reassortment

9. Serious Bunya Virus Diseases
Crimean Congo Hemorrhagic Fever Virus
Rift Valley Fever Virus
La Crosse Virus
Hanta Virus

10. Pathogenesis Early fever & viremia Late encephalitis retinitis
renal involvement

12. Epidemiology Determined by distribution of vector and mammalian host
Humans: accidental dead end hosts

13. Diagnosis Presumptive Confirmation febrile illness
geographic site of exposure
Sean
vectors
Confirmation
virus isolation
Virus specific IgM
Rise in antibody titer

14. Control Control Vectors arthropods rodents Vaccination
Humans: Crimean Congo
Sheep & Cattle : Rift Valley

15. Crimean Congo Hemorrhagic Fever Virus
Headache
pain in limbs
bleeding from multiple orifices

16. Rift Valley Fever Virus
Explosive potential
Egypt 1977
200,000 cases
600 deaths

17. La Crosse Virus
Fever
convulsions
drowsiness
focal neurological signs

18. Hanta Virus
Rodents - transmission to humans via aerosolized excretions and bites. A wide variety of biting insects.
Hanta virus Web site

19. Hanta Virus Lipid envelope susceptible to most disinfectants.
Need to lower pH < 5

20. Hanta Virus tripartite negative-sense RNA
L,>>viral transcriptase
M>> envelope glycoproteins
>. nucleocapsid protein

21. Hantavirus Pulmonary Syndrome
febrile prodrome
followed by
non-cardiogenic pulmonary edema,
hypotension
shock

22. Hantavirus Four corners New Mexico April 5, 1994 68 cases in 17 states
May deaths
April 5, cases in 17 states
Deermouse in New Mexico - principal reservoir do not cause infections - found in saliva, urine, feces and is shed sporadically
-infected animals - blood, organs, saliva feces, urine.

23. Hanta virus ...continued
Humans visiting holding areas for animals can become infected in five minutes.
outbreaks of this and associated diseases have been observed in Baltimore, Maryland Soeul, Korea Belgium England

24. DONE!!!