Download presentation
Presentation is loading. Please wait.
Published byThomas Turner Modified over 9 years ago
1
Regulation of endothelial MAPK/ERK signalling and capillary morphogenesis by low-amplitude electric field by Abdul Q. Sheikh, Toloo Taghian, Bryan Hemingway, Hongkwan Cho, Andrei B. Kogan, and Daria A. Narmoneva Interface Volume 10(78):20120548 January 6, 2013 ©2013 by The Royal Society
2
Experimental set-up for microvascular endothelial cells exposure to EF. (a) High-frequency (7.5 GHz) EF set-up: an insert with endothelial cells was placed in the small plastic dish inside the cavity resonator to which EF was delivered from a vector network... Abdul Q. Sheikh et al. J. R. Soc. Interface 2013;10:20120548 ©2013 by The Royal Society
3
Electric field enhances angiogenic response by microvascular endothelial cells. Abdul Q. Sheikh et al. J. R. Soc. Interface 2013;10:20120548 ©2013 by The Royal Society
4
Angiogenic effects of EF do not require VEGF binding to VEGFR2 receptor. Abdul Q. Sheikh et al. J. R. Soc. Interface 2013;10:20120548 ©2013 by The Royal Society
5
MAPK/ERK pathway is involved in EF-mediated angiogenic response. Abdul Q. Sheikh et al. J. R. Soc. Interface 2013;10:20120548 ©2013 by The Royal Society
6
High-frequency EF increases the phosphorylation of ERK, but not JNK or p38 MAP kinase in microvascular endothelial cells. Abdul Q. Sheikh et al. J. R. Soc. Interface 2013;10:20120548 ©2013 by The Royal Society
7
High-frequency EF enhances MEK phosphorylation and MEK–cRaf complex formation in microvascular endothelial cells. Abdul Q. Sheikh et al. J. R. Soc. Interface 2013;10:20120548 ©2013 by The Royal Society
8
Effects of PI3K and eNOS inhibition and Ca2+ chelation on EF-mediated MEK phosphorylation: (a) addition of PI3K inhibitor LY294002 did not abolish EF-induced increase in pMEK levels, when compared with no-EF controls (n = 4, p < 0.05), suggesting that this... Abdul Q. Sheikh et al. J. R. Soc. Interface 2013;10:20120548 ©2013 by The Royal Society
9
In vivo exposure to high-frequency EF enhances VEGF expression in diabetic wounds: (a) In vivo EF exposure set-up consisted of two antennae, which were connected to the EF source through a flexible co-axial cable. Abdul Q. Sheikh et al. J. R. Soc. Interface 2013;10:20120548 ©2013 by The Royal Society
10
(a) Angiogenic signalling pathways and (b) proposed mechanism for high-frequency EF regulation of angiogenic response. Abdul Q. Sheikh et al. J. R. Soc. Interface 2013;10:20120548 ©2013 by The Royal Society
Similar presentations
© 2025 SlidePlayer.com. Inc.
All rights reserved.