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Published byOsborne Cummings Modified over 9 years ago
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DEPARTMENT OF CELL BIOLOGY Head: Dr. Jacek Bigda, Associate Professor Research Staff: Dr. Małgorzata Doszczak Dr. Patrycja Koszałka Dr. Zuzanna Dobrzańska Ph.D. Students: Marcin Okrój Arkadiusz Pierzchalski
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MAIN TOPIC Mechanism of action of tumor necrosis factor (TNF) in vitro & in vivo TEACHING cell biology, histology, immunology
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DEPARTMENT OF CELL BIOLOGY research interests: mechanism of IL-1-induced cytotoxicity; intracellular signaling of tumor necrosis factor; mechanism of toxicity of the anti-angiogenic fumagillin derivative, TNP-470; mechanism of anti-tumor action of poly-RGD peptides; mechanism of accelerated growth of hamster melanoma following LPS application.
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U 937 – human histiocytic lymphoma TNF-R55 F N TF N TF N T TNF-R75 F N T m F N T m F N T
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Cycloheximide (CHX) U937 cell variants
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U 937 M U 937 G U 937 ATCC
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IL-1 cytotoxicity
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control 4 h 2 h 0,5 h 1 h 0 100 200 300 400 500 TNF [pg/ml] control CHX IL-1 IL-1/CHX IL-1 cytotoxicity
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Isotype control Untreated IL-1/CHX
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DEPARTMENT OF CELL BIOLOGY financial support: Analysis of mechanism regulating secretion of TNF following application of interleukin 1 and cycloheximide; State Committee for Scientific Research, Poland (2002-2005); Mechanism of synergistic anti-tumor action of tumor necrosis factor (TNF) and angiogenesis inhibitor TNP-470; State Committee for Scientific Research, Poland (2001-2004); Analysis of TNF sensitivity of variants of the U937 cell line; Intramural grant, Medical University of Gdańsk (2002-2003); Modulation of tumor growth by means of cytokines; Core fund, Intercollegiate Faculty of Biotechnology UG-MUG;
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DEPARTMENT OF CELL BIOLOGY selected previous grants: Significance of tumor necrosis factor and its receptors for growth and dissemination of Bomirski melanoma; State Committee for Scientific Research, Poland (1994-1997); Immunomodulatory and anti-tumor activity of cloned and purified rat tumor necrosis factor; State Committee for Scientific Research, Poland (1997-2000); Influence of receptors of tumor necrosis factor for apoptosis of cancer cells; State Committee for Scientific Research, Poland (1997-2000); Mechanism of cytotoxic action of interleukin 1; State Committee for Scientific Research, Poland (1999-2002); TNF gene polymorphism and humoral reactivity against p53 protein in patients suffering from non-small cell lung cancer; State Committee for Scientific Research, Poland(1999-2002).
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Tri-City Central Animal Laboratory, Medical University of Gdańsk
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Tri-CityCentral Animal Laboratory, Medical University of Gdańsk Tri-City Central Animal Laboratory, Medical University of Gdańsk
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