1. OnSite
Malaria Rapid Test

2. Current Malaria Diagnostic Methods
Detection of
Advantage
Disadvantage
Thick smear microscope
Parasites
Detects parasites /uL
Golden standard
Need good microscope
Prone to human errors
Rapid Ag test
Parasite specific antigen
Detects parasites/ uL
Simple procedure, less errors
Be used at any place, any time
The most popular screening test
Need to bring the sensitivity up to
10-50 parasites /uL
Rapid Ab test
Antibodies to malaria parasites
Be used to track malaria infection
history: blood donor screening;
vaccine candidate screening
Can not differentiate current or past infection
ELISA Ab test
Antibodies to malaria infection
Molecular diagnosis
Malaria gene
Very sensitive
To be used by researcher now
Expensive equipments
Well trained lab tech
No product on market

3. Malaria Ag Rapid Test on Market
Malaria parasite specific LDH antibody by Dr. Makler
Pf HRP-II antibody by S. African institute
Aldolase antibody
Pv LDH
Pf LDH
Pan LDH
Pf LDH
Pv LDH
HRP-II
Pan LDH
HRP-II
Aldolase
HRP-II
Aldolase
Pf-LDH
Popular
The most popular
Least popular
HRP-II Clone C1-13 cross-reacts
to high concentration of LDH
Majority tests use Dr. Makler’s clones
Supply security issue due to acquisition !

4. About Dr. Makler
An American doctor studied blood viscosity ended to spend his rest of career in malaria diagnosis
Discovered malaria specific LDH
Generated dozes of malaria parasite specific LDH antibody
Licensed antibodies to “Optimal” manufacturer
- Optimal was dominated the malaria Ag rapid test on market for a while
Breakdown the deal with “Optimal”
- Malaria Ag rapid test can be made by many other manufacturers!
Sold the antibodies to a major malaria Ag test manufacturer in 2011!
Supply can be interrupted at any time
Price can be up at any time
Many companies and distributors are at the mercy of “the new owner”

5. Why it is difficulty to make malaria antibody?
LDH is not a stable protein
No resource of native antigens
Most recombinant protein conformation are quite different than the native protein
Many companies tried, failed to replace Makler’s antibody

6. CTK’s Advantage
Have E.Coli, insect, and mammalian express system to fit variant needs
Newly improved monoclonal antibody system generates high affinity antibody in 2 months
Newly improved polyclonal antibody system generates high affinity antibody in 2 months

7. CTK Solution
Has made a specific anti HRP-antibody that dose not cross react to LDH
Has just developed LDH antibody

8. CTK Test WHO Evaluation Result Overview
Round 1
Missed
Pf –HRP Rapid Test:
Met request
WHO procurement list
Round 2
A new chemical used
in conjugate pad
Round 3
Pf - Pan Ag Rapid Test
Pf - Pv Ag Rapid Test
False positive
Round 4
Pf - Pan Ag Rapid Test
Pf - Pv Ag Rapid Test
Result
Pending
In a 200 case field study:
specificity is > 95%

9. CTK – A Complete Resource of Malaria Reagents
Recombinant Pf & Pv antigen for Ab detection
Pf-MSP-1 antigen
Pv-MSP-1 antigen
Malaria Ab ELISA
RecombiLISA Malaria Pf Ab ELISA*
RecombiLISA Malaria Pv Ab ELISA*
* Under development
OnSite Pf - Pv Ag Rapid Test
OnSite Pf - Pan Rapid Test
OnSite Pf Rapid Test
Malaria Ag Rapid Test
Positivia HRP-II control
Positivia Pf-LDH control
Positivia Pv-LDH control
Positivia Aldolase control
Malaria Ag Test Control

10. CTK -the only supply for your needs in combating malaria
To make tests To diagnose malaria To test product quality To monitor malaria eradication To screen malaria vaccine candidate

11. Smear Test False Result
Low density of parasites
less skillful technician
Confusion with bacterial,
fungi, dirt cell debris
False Positive
Poor microscope quality
Poor blood film preparation
Not sufficient fields checked
False Negative
Poor staining
Less skillful technician
Confusion with bacterial,
fungi, dirt cell debris
Errors in species identification

12. Rapid Test False Result
False Positive
RF & heterophile antibody
Low parasite density
Mutation of HRP-II gene
Unknown inhibitor in blood
False Negative
Errors in species identification
Cross reaction of antibody used

13. Early Diagnosis for a better life Together, we grow Athenese Dx & CTK

14. Together, we grow Athenese Dx & CTK
Athenese DX contact information:
Module # 407 & 408, 4th Floor,
Ticel Bio Park II, No. 5, CSIR Road,
Taramani, Chennai ,
TamilNadu, India.
Together, we grow Athenese Dx & CTK

15. Athenese DX contact information
Registered Office
Ticel Manufacturing
BGB Manufacturing
San Diego R&D office