Presentation is loading. Please wait.

Presentation is loading. Please wait.

ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Recent Trends in estimated HIV-1.

Published byClara Cain Modified over 9 years ago

Similar presentations

Presentation on theme: "ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Recent Trends in estimated HIV-1."— Presentation transcript:

1 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Recent Trends in estimated HIV-1 Incidence among Royal Thai Army (RTA) conscripts from 2005-2009 in Thailand Major Sutchana Tabprasit Armed Forces Research Institute of Medical Sciences (AFRIMS), Royal Thai Army (RTA), Bangkok, Thailand

2 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Primary HIV-1 Infection Acute infection Rapid viral replication 80-90% develop an acute syndrome characterized by flu-like symptoms Broad HIV-1 specific immune response

3 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Recent HIV-1 Infection (Incidence) Difference of HIV-1 antibody in various aspects - Titer - Proportion - Isotype (avidity)

4 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Adults and children estimated to be living with HIV, 2008 UNAIDS. 2009 Report on the global AIDS epidemic

5 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Estimated number of adults and children newly infected with HIV, 2008 UNAIDS. 2009 Report on the global AIDS epidemic

6 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Pre-seroconversion HIV-1 RNA / DNA p24 Antigen Methods for Detection Early HIV Infection (1)

7 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Post-seroconversion Less sensitive EIAs IgG-capture BED-EIA Avidity assays Anti-p24 IgG3 Antibodies Methods for Detection Early HIV Infection (2)

8 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Advantages of HIV-1 Incidence To monitor the effectiveness of the national prevention and control programs To determine active area of HIV transmission To design the possibility to conduct HIV vaccine trail

9 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria -To estimate the HIV-1 incidence among the Royal Thai Army (RTA) conscripts Objective

10 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Thailand

11 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Recruitment of young Thai males occurs in April each year 60,000 Males/year

12 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria HIV-1 SEROPREVALENCE OF YOUNG MEN ENTERING THE ROYAL THAI ARMY 1991-2009 4.5 0 0.5 1 1.5 2 2.5 3 3.5 4 1991199219931994199519961997199819992000200120022003200420052006200720082009 Year HIV-1 Seroprevalence 2.9 3.5 3.7 3.0 2.4 2.1 1.9 1.6 1.3 1.2 0.7 0.6 0.5

13 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria The dot density of seroprevalence among recruits in the Royal Thai Army (RTA), 2005-2009. Positive= 1,316 dots Prevalence= 1,316/268,504 (0.5%) 1 dot = 1 prevalent case

14 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria HIV Seropositive Detection From 2005-2009 RNR ELISA I PositiveNIndeterminate WB/ ELISA II and III BED-EIA 1,316 268,504 149

15 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria IgG- Capture HIV-EIA

16 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Principles of IgG- Capture HIV-EIA 96-well plate is coated with goat anti-human IgG

17 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria 1. add specimen IgG antibodies from plasma/serum 2. add Biotinylated HIV-1 BED peptide (gp41) 3. add Streptavidin–HRP Conjugate 4. add substrate Principles of IgG- Capture HIV-EIA

18 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Read at wavelength 450 nm with reference wavelength 630-650 nm Spectrophotometer Plate Principles of IgG- Capture HIV-EIA

19 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Assay Validation : Run Control R2R2 OD-N = OD/ Median OD of Calibrator

20 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria The Algorithm of IgG Capture HIV-EIA Confirmed HIV-seropositive specimens Test in single If ODn > 1.2 Long-term Seroconversion If ODn ≤ 1.2 Repeat test in triplicate (Confirmatory) If Median ODn > 0.8 Long-term Seroconversion If Median ODn ≤ 0.8 Recent Seroconversion

21 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Results Figure 1: HIV incidences at time of entry into the RTA 2005-2009 Percent per year 200520062007 20082009 0.14 0.19 0.20 0.26 0.25 Year

22 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Table 1: HIV-1 incidence among the RTA Conscripts From 2005-2009 by demographic factors Demographic Factors Number of Conscripts Number of HIV-1 positive (Seroprevalence) Number of BED-CEIA positive Incidence (95% confidence interval) Age 18 – 2026,67687 (0.33)270.19(0.12 – 0.27) 21190,360828 (0.43)2030.20 (0.17 – 0.22) 22 – 3040,266335 (0.83)700.33 (0.25 – 0.40) Education 0 – 668,131471 (0.69)960.25 (0.20 – 0.30) 7 – 988,096379 (0.42)790.15 (0.12 – 0.19) >9102,274406 (0.38)1270.25 (0.21 – 0.29) Marital Status Single209,6701,057 (0.50)2600.24 (0.21 – 0.27) Married47,525198 (0.42)420.13 (0.09 – 0.17)

23 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Summary  Increasing trend of HIV incidence in the recent years alerts the active program for HIV prevention and control programs in Thailand  HIV incidence was higher in recruited subjects with older age. (22-30 years)  IgG capture BED is a promising predictor to estimate HIV incidence in the future.

24 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Acknowledgement Conscripts of the Royal Thai Army Armed Forces Research Institute of Medical Sciences (AFRIMS) Army Institute of Pathology, King Mongkut Medical Center Bureau of Epidemiology, Department of Disease Control of Ministry of Public Health (MOPH) Thailand MOPH - U.S. Centers for Disease Control and Prevention (CDC) Collaboration

25 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Thank you so much

26 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria

27 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria

28 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria

29 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria

30 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria

31 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria

32 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria www.nrl.gov.au

33 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Early HIV Infection Adapted from Parekh BS, McDougal JS. Indian J Med Res 2005;121(4):510-518.

34 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Methods for Estimating HIV Incidence www.nrl.gov.au

35 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria 155 days Level Time since infection Difference between BED-CEIA and HIV screening test

36 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria BED Branch Peptide Antigen

37 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Prevalence Formula Number of HIV-1 infection Number of total in the survey 100 P =

38 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Adjustment of Incidence Formula R -ɛP (R/2) + N(w/365) - ɛN - ɛP/2) W=155 days R=Total testing recent in the BED-CEIA N=Total testing HIV negative P=Total testing HIV positive ɛ =False recent rate in those with ling term (>2weeks) infection 100I =

39 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Plate Plan For Sample

40 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Plate Plan For Sample

41 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Acceptable ODn Ranges of Controls and Calibrator Acceptable Median OD Ranges of Controls and Calibrator

42 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Highly active antiretroviral therapy (HAART)  Combination chemotherapy for HIV infection consisting of reverse transcriptase inhibitors and a viral protease inhibitor.  Produce marked increase in peripheral blood CD4 + T cells and decreases in HIV plasma RNA copy number.  Reduce plasma virus titers to below detectable levels for more than 1 year and slow the progression of HIV disease.

43 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria

44 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria

45 ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Specificity Adjustment (Hargrove et al)

Download ppt "ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES XVIII International AIDS Conference, July 18-23 2010, Vienna Austria Recent Trends in estimated HIV-1."

Similar presentations

Nick Curry, MD, MPH Infectious Diseases Prevention Section

Nick Curry, MD, MPH Infectious Diseases Prevention Section
Diagnosis and Management of Acute HIV Infection HIV Clinical Guidelines from the New York State Department of Health AIDS Institute January 2010 HIV CLINICAL.

Diagnosis and Management of Acute HIV Infection HIV Clinical Guidelines from the New York State Department of Health AIDS Institute January 2010 HIV CLINICAL.
Satellite meeting on the STARHS HIV diagnostic assay in association with the 15th International AIDS Conference July 2004, Bangkok, Thailand Robert S.

Satellite meeting on the STARHS HIV diagnostic assay in association with the 15th International AIDS Conference July 2004, Bangkok, Thailand Robert S.
At this presentation we introduce a new challenge to developing a novel treatment for HIV Under Title the Antibodies of Reverse Transcriptase System.

At this presentation we introduce a new challenge to developing a novel treatment for HIV Under Title the Antibodies of Reverse Transcriptase System.
Unit 6 Diagnosis & Follow-up of HIV Infection

Unit 6 Diagnosis & Follow-up of HIV Infection
CHER Trial: Early Antiretroviral Therapy and Mortality Among HIV- Infected Infants New England J Med 2008;359 (21): 2233-2244.

CHER Trial: Early Antiretroviral Therapy and Mortality Among HIV- Infected Infants New England J Med 2008;359 (21):
Pediatric Diagnosis of HIV-1 Infection Using Dried Blood Spots Chin-Yih Ou, PhD NCHSTP/DHAP Centers for Disease Control and Prevention.

Pediatric Diagnosis of HIV-1 Infection Using Dried Blood Spots Chin-Yih Ou, PhD NCHSTP/DHAP Centers for Disease Control and Prevention.
Using longitudinal, population-based HIV surveillance to measure the real-world impacts of ART scale-up in KwaZulu- Natal, South Africa Frank Tanser Presentation.

Using longitudinal, population-based HIV surveillance to measure the real-world impacts of ART scale-up in KwaZulu- Natal, South Africa Frank Tanser Presentation.
Impact of Age and Race on New HIV Infections among Men who have Sex with Men in Los Angeles County 2008-2010 Shoshanna Nakelsky, MPH Division of HIV and.

Impact of Age and Race on New HIV Infections among Men who have Sex with Men in Los Angeles County Shoshanna Nakelsky, MPH Division of HIV and.
Spectrum 2007 Overview and New Changes UNAIDS Reference Group on Estimates, Model and Projections 2007.

Spectrum 2007 Overview and New Changes UNAIDS Reference Group on Estimates, Model and Projections 2007.
Laboratory Training for Field Epidemiologists Sensitivity and specificity Predictive values positive and negative Interpretation of results Sep 2007.

Laboratory Training for Field Epidemiologists Sensitivity and specificity Predictive values positive and negative Interpretation of results Sep 2007.
12/6/07 v.3CDC 2007 HIV Diagnostic Conference1 Diagnosis of HIV-1 Infection in Phase I & II HIV Vaccine Trials RW Coombs 1, J Dragavon 1, B Metch 2, CJ.

12/6/07 v.3CDC 2007 HIV Diagnostic Conference1 Diagnosis of HIV-1 Infection in Phase I & II HIV Vaccine Trials RW Coombs 1, J Dragavon 1, B Metch 2, CJ.
An Introduction to HIV Incidence Surveillance (HIS) in California California Department of Public Health Office of AIDS.

An Introduction to HIV Incidence Surveillance (HIS) in California California Department of Public Health Office of AIDS.
Use of avidity reagent. Panbio Buffered Avidity Reagent Mild protein-denaturing solution that may be useful in differentiating recent infections from.

Use of avidity reagent. Panbio Buffered Avidity Reagent Mild protein-denaturing solution that may be useful in differentiating recent infections from.
Routine HIV Screening in Health Care Settings David Spach, MD Clinical Director Northwest AIDS Education and Training Center Professor of Medicine, Division.

Routine HIV Screening in Health Care Settings David Spach, MD Clinical Director Northwest AIDS Education and Training Center Professor of Medicine, Division.
Wisconsin HIV/AIDS Surveillance Annual Review: Slide Set New diagnoses, prevalent cases, and deaths through December 2014 April 2015 P-00484 Wisconsin.

Wisconsin HIV/AIDS Surveillance Annual Review: Slide Set New diagnoses, prevalent cases, and deaths through December 2014 April 2015 P Wisconsin.
Primary HIV Infection: the CDC study Pragna Patel, MD MPH Medical Epidemiologist Behavioral and Clinical Surveillance Branch DHAP, CDC February 28, 2005.

Primary HIV Infection: the CDC study Pragna Patel, MD MPH Medical Epidemiologist Behavioral and Clinical Surveillance Branch DHAP, CDC February 28, 2005.
Dr. Laura Guay Vice President for Research Elizabeth Glaser Pediatric AIDS Foundation J2J Global Media Training on HIV/AIDS July 14, 2010 Vienna, Austria.

Dr. Laura Guay Vice President for Research Elizabeth Glaser Pediatric AIDS Foundation J2J Global Media Training on HIV/AIDS July 14, 2010 Vienna, Austria.
Trends in HIV prevalence and HIV incidence in South Africa Thomas M. Rehle, MD, PhD Human Sciences Research Council (HSRC) Cape Town, South Africa AIDS.

Trends in HIV prevalence and HIV incidence in South Africa Thomas M. Rehle, MD, PhD Human Sciences Research Council (HSRC) Cape Town, South Africa AIDS.
Enhancing HIV/AIDS Surveillance in California California Department of Public Health Office of AIDS Guide for Health Care Providers.

Enhancing HIV/AIDS Surveillance in California California Department of Public Health Office of AIDS Guide for Health Care Providers.

Similar presentations

Ads by Google