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Published byRoberta Williamson Modified over 9 years ago
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long polymers (thousands) of nucleotides therefore, sometimes called polynucleotides each nucleotide consists of 3 parts 1.a phosphate group 2.a pentose sugar called deoxyribose in DNA (or ribose in RNA) 3.in DNA/RNA, five nitrogen bases (only need to recognize these) DNA - thymine(T), cytosine(C), adenine(A) and guanine(G) RNA – uracil(U) is substituted for thymine
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Warning: Lots of pictures of nucleotides
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DNA RNA
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Condensation reaction the joining of nucleotides with a phosphodiester bond (a covalent bond) to form a sugar-phosphate backbone
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RNADNA sugar is ribose (OH is on 2’ carbon) sugar is deoxyribose (H is on 2’ carbon) contains the base uracilcontains the base thymine single strandedtwo strands forming a double helix held together by hydrogen bonds Distinguish between RNA and DNA
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complementary base pairing the size and shape of the bases is such that hydrogen bonds can only form at specific sites adenine only forms two hydrogen bonds with thymine guanine only forms three hydrogen bonds with cytosine why twisting/spiraling of DNA? it minimizes the repulsion between negatively charged phosphates
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inherit genetic material from parents genetic info. on DNA allows proteins to be made through the process of transcription and translation
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Replication http://highered.mcgraw- hill.com/sites/0072437316/student_view0/chapter14/animations.html http://www.johnkyrk.com/DNAreplication.html
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Transcription and Translation http://highered.mcgra w- hill.com/sites/007243 7316/student_view0/ chapter15/animations.html
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64 codons that code for 20 amino acids or to stop
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commonly known as ‘DNA fingerprinting’ method used to identify individuals by similarities or differences in their DNA isolation of the DNA from a sample source blood, semen, skin cells, hair or saliva DNA is the cut up with restriction enzymes and replicated these DNA fragments are then separated into bands according to size using gel electrophoresis the resulting pattern is transferred to a nylon membrane, labeled with radioactive 32 P, and exposed to x-ray film film is finally compared to other samples of DNA
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