1. Nov.2008 Miss.TAHANI AL-SHEHRI Haemoglobin ( Hb) & anaemia

2. Objective To estimate the Hb concentration in the blood sample. To assess the condition of patient by such an estimation. Further experience in the use of kits.

3. Introduction and principle Haemoglobin

4. Introduction and principle Haemoglobin

5. Rate of Hb Synthesis & RBCs Formation related to Oxygen content of blood O 2 content Hb Syn Ex. Anoxia / Hypoxia Blood loss or Hemolysis Hb synthesis 6-8 folds Anaemia Hb synthesis

6. Erythropoieosis Synthesis of erythrocyte Tack place in bone marrow erythrocyte are derived from primitive nucleated cell in bone marrow by successive process of mitosis & maturation.

7. Factor required to manufacture RBC Varity of hormone Trace metals Enzymes Co enzymes Essential a.a e.g glycine Iron

8. Hb Catabolism

9. Anemia State in which the Hb conc. in blood less than the normal level. Normal level of Hb : Man 800g =14-18g/dL Woman 600g =12-16g/dL Causes of anemia : Excessive blood loss due to bleeding rate of erythrocyte destruction (hemolytic anemia ) rate of erythrocyte production

10. Classification of anemia Dyshemooietic anemia Hemolytic anemia Iron deficiency anemia Megaloblastic anemia Membrane defect Enzyme Deficiency