1. Plant Physiology 3(2-1) An Overview of Plant Physiology

2. Educational Objectives of this Course The main objective is to gain an understanding of general and advance physiological processes that occur in most of the plants.

3. Plant Physiology Practical Course Outline Introduction to equipment used in Plant Physiology lab Preparation of chemical solutions and media for plant growth Seed germination and effect of various factors on germination and plant growth Isolation of Chlorophyll content In silico analysis of plant DNA sequences and primer designing DNA extraction from plant leaves Agarose gel electrophoresis Polymerase chain reaction Determination of transpiration in plant leaf Leaf structure comparison of C3 and C4 plants

4. From smallest to largest plants

5. What is plant physiology? PHYSIOLOGY: study of the function of cells, tissues, organs of living things; and the physics/chemistry of these functions… “Structure correlates to function”

6. Plant Anatomy: Cells Plant cells are basic building blocks Can specialize in form and function By working together, forming tissues, they can support each other and survive Levels of organization cells > tissues > organs > whole plant

7. Plant Tissues Types All plant organs (roots, stems, leaves) are composed of the same tissue types. There are three types of tissue: 1. Dermal – outermost layer 2. Vascular – conducting tissue, transport 3. Ground – bulk of inner layers

8. 1. Dermal tissue Epidermis is the outermost layer of cells Like the “skin” of animals In stems and leaves, epidermis has cuticle, a waxy layer that prevents water loss. Some have trichomes, hairs. Root epidermis has root hairs, for water and nutrient absorption

9. Preformed Resistance Mechanisms Any biological or physical barrier that is always present in plants for protecting them against different stress factors. Structural barriers Epidermis---The first line of defense Wax layer on cuticle leaves and fruits Cuticle (Cutin+Cellulose+Pectin) Thin in aquatic plants while thick in desert (Cacti). Hydrophobic, doesn’t Let the water stand on surface that is helpful for fungal spore germination. Here I come!! THE PATHOGEN Fig: The epidermis is a protective layer of cells that is usually covered with a thin, water- resistant cuticle that helps prevent water loss from the plant.

10. 2. Vascular tissue Transports water and organic materials (sugars) throughout the plant Xylem – transports water and dissolved ions from the root to the stem and leaves. Phloem – carries dissolved sugars from leaves to rest of the plant

11. Xylem Transports water and dissolved minerals Tracheids: long, thin tube like structures without perforations at the ends Vessel elements: short, wide tubes perforated at the ends (together form a pipe, called vessel). Both cells have pits (thin sections) on the walls TracheidsVessel elements

12. Xylem cells Xylem cells are dead! They are hollow cells and consist only of cell wall

13. Phloem Cells that transport organic materials (sugars) Phloem cells are ALIVE! (unlike xylem) However, they lack nucleus and organelles

14. Phloem: transports sugars Phloem composed of cells called sieve tube members (STM) Companion cells join sieve tube members, are related, and help to load materials into STM End walls of STM have large pores called sieve plates Sieve tube member Companion cells Sieve plates

15. 3. Ground tissue Makes up the bulk of plant organs. Organs: tissues that act together to serve a specific function Functions: Metabolism, storage and support.

16. Plant Organs Leaves Stems Roots Dermal Vascular Ground Dermal Vascular Ground Dermal Vascular Ground

17. Functions of plant organs: ROOTS: Anchorage, water/nutrient absorption from soil, storage, water/nutrient transport STEMS: Support, water/nutrient transport LEAVES: Photosynthesis (food production)

18. ROOTS ROOTS “the hidden half” Functions of roots: Anchorage Absorption of water & dissolved minerals Storage (surplus sugars, starch) Conduction water/nutrients Attracts beneficial microbes and fungi

19. Anatomy of a root epidermis cortex vascular

20. Root Epidermis Outermost, single layer of cells that: – Protects (from diseases) – Absorbs water and nutrients ROOT HAIRS: tubular extensions of epidermal cells. Increase surface area of root, for better water/nutrient absorption

21. Root Hairs: water and mineral absorption Root hairs increase surface area for better absorption

22. Root Cortex Stores starch, sugars and other substances

23. Root Ground tissue In roots, ground tissue (a.k.a. cortex) provides support, and often stores sugars and starch (for example: sweet potato etc.) cortex

24. Root Cortex: Endodermis Endodermis: the innermost layer of the cortex

25. Root cortex: Casparian strip The Casparian strip is a water-impermeable strip of waxy material found in the endodermis (innermost layer of the cortex). The Casparian strip helps to control the uptake of minerals into the xylem: they have to go through the cytoplasm of the cell!

26. STEMS Above-ground organs (usually) Support leaves and fruits Conduct water and sugars throughout plant (xylem and phloem)

27. Stem anatomy Dermal, ground and vascular tissues… pith cortex epidermis Vascular bundles

28. Types of stems Herbaceous vs. Woody stems

29. Tissues of stems Epidermis (Dermal tissue type) Provides protection Has cuticle (wax) prevents water loss Trichomes (hairs) for protection, to release scents, oils, etc.

30. Stem Vascular tissue Vascular bundles – composed of both xylem and phloem Xylem – Conducts water – Support Phloem – Conducts food – Support Vascular cambium

31. Vascular cambium Occurs in woody stems Vascular cambium located in the middle of the vascular bundle, between xylem and phloem

32. Vascular tissue: Trees Vascular tissue is located on the outer layers of the tree. wood phloem xylem bark Vascular cambium

33. Vascular tissue forms rings in trees Annual rings: xylem formed by the vascular cambium during one growing season One ring = one year

34. History of the tree: annual rings 1489: Tree is planted by Native American 1492: Columbus lands in the Americas 1776: Declaration of US independence 1861: Start of Civil War 1969: Man lands on Moon 1917 & 1945: Tree Survives two World Wars 1971: Birth Year of the IDIOT who cut down this tree!!! Dendrochronology : tree time-keeping

35. LEAVES: ‘Photosynthetic factories’ of the plant… Function: Photosynthesis – food production for the whole plant Blade: Flat expanded area Petiole: stalk that connects leaf blade to stem, and transports materials BLADE

36. Leaf Anatomy Leaf anatomy is correlated to photosynthesis: Carbon dioxide + Water  sugars + oxygen dermal ground vascular dermal

37. Leaf epidermis Is transparent – so that sun light can go through. Waxy cuticle protects against drying out Lower epidermis: stomata with guard cells – for gas exchange (CO 2, H 2 O in; O 2 out)

38. Leaf epidermis Stomata Guard cells

39. Leaf vascular tissue VEINS  vascular tissue of leaves. Veins are composed of xylem (water transport) phloem (food transport) and bundle sheaths, cells surrounding the xylem/phloem for strength & support

40. Leaf Mesophyll Middle of the leaf Composed of photosynthetic ground cells: Palisade parenchyma (long columns below epidermis; have many chloroplasts for photosynthesis, Chlorenchyma) Spongy parenchyma (spherical cells) with air spaces around, (for gas exchange)

41. Plant Hormones Chemical compounds produced by plants Effective at very low concentrations Five major hormone groups are: 1.Auxins 2.Gibberellins 3.Cytokinins 4.Abscisic Acid 5.Ethylene

42. 1. AUXINS Promote cell growth Involved in gravitropism and phototropism Control fruit development

43. 2. Gibberellins Promote stem elongation 3. Cytokinins Promote cell division and organ differentiation 4. Abscisic Acid Promotes seed dormancy Causes stomata closing

44. 5. ETHYLENE Gaseous hormone, Ethylene promotes fruit ripening! AirEthylene

45. Recommended Book Plant Physiology by Taiz and Zeiger 3 rd Edition Available in ASAB Library