1. Erie’s Lab Zimeng Li 2012-2-29

2. Content Questions Feasibility Problems and Solutions Video/Fluid AFM

3. Questions Sliding clamp model Improve image resolution Study orders in the pathway Differentiation of protein complex What else?

4. Feasibility Physical blockage AFM scanning speed

5. Known Problems and Solutions Water – constantly drying Injection of reactants Constant translational movement

6. Water – constantly drying Closed Fluid Cell Drop water

7. Injection of reactants Closed Fluid Cell Needle Injection

8. Constant translational movement

9. Glue Magnet Simultaneously move scan area

10. Video AFM Fluid AFM

11. Video AFM

12. Fluid AFM Samples still attached to surface Tips interacting with sample Cantilever drive frequency is coupled with fluid relaxation frequency

13. To-dos