1. Microbiology Overview KWWOA April 09, 2014 Department for Environmental Protection Energy & Environment Cabinet

2. Topics

3. o Proficiency Tests o Chain of Custody o Methods o Membrane Filtration

4. Proficiency Tests

5. o Drinking Water: WS Study SW sample for MPN o Wastewater: DMR-QA WP Study o KY Approved PT Providers List

8. Proficiency Tests

11. Revised reports will not be accepted. Photo: peoplesdaily.com

12. Chain of Custody

13. Client name & address Sampler name Sample location Collection date & time Sample type (grab/composite) Matrix (DW/WW) Field sample ID Laboratory sample ID Container type, & size/volume Number of containers Preservative (ice, etc.) Field data (pH, TRC, etc.) Requested analysis Reason for rejection or special remarks Signature, date & time for each transaction (relinquishing & receiving) Sample temperature upon arrival at lab

14. BARF is NOT a COC

15. Methods

16. Microbiology Methods MethodDrinking WaterWastewater Colilert E. coli Colilert-18 E. coli Fecal – ATP @ 44.5 o C Colisure Coliscan mFC NA-MUGFecal mEndo EC-MUG mColiBlue-24 E. coli Modified mTEC E. coli

17. 17 Colilert-18 P/A Colilert-18 pre-warming

18. 18 Colilert-18 MPN

19. Membrane Filtration

20. Dilutions: Select appropriate volumes Fecal Coliform or E. coli colonies: 20-60* Total colonies: 200 *Use range as specified in particular method. Photo: www.pall.com

21. Membrane Filtration Blanks: Beginning of series End of series RECORD results of blanks

22. Membrane Filtration Calculating Results: Use dilution that is in range to determine result. If multiple plates within range, average those plate counts. If zero colonies on all plates, calculate as if 1 colony on filter from largest volume processed. Include remark “<“ in comments. Photos: www.avocetlab.com

23. Membrane Filtration Calculating Results: If all plates are above range, calculate using filter from smallest volume processed. Include remark “Estimated“ in comments. WW TNTC = 60,000

24. Contact Information Tiffany Nolan Kentucky Department for Environmental Protection Division of Water 200 Fair Oaks Lane; 4 th Floor Frankfort, KY 40601 Phone: (502) 564-3410 Email: Tiffany.Nolan@ky.gov