1. UNIT 2: MICROBIOLOGY, SAFETY AND INFECTION CONTROL Objectives: Identify the 5 types of microorganisms Identify the five factors that effect microbial growth Explain the transmission of disease and methods of transmission Identify the signs and symptoms of infection Review CDC Immunization schedule and discuss rationale Compare and contrast the pros and cons of vaccinations

2. TO DO CHECKLIST Microbiology lab write up Chain of Infection diagram (p. 75- 77) Factors of microbial growth chart (p.66-71) Vaccine Research graphic organizer with personal reflection attached. (1 page typed) Study for test (On Wednesday 10/22/14)

3. MICROBIOLOGY LAB WRAP-UP Discuss findings as a class/results. Analysis: Explains data in detail with examples. Explains trends etc. Questions to consider when writing your conclusion: What places in the school appeared to be the cleanest? What places in the school appeared to be the dirtiest? What sources of error were present in this experiment? What would you do differently if you were to repeat the experiment? What recommendations could you make for a cleaner school environment?

4. MICROBIOLOGY Microbiology- The study of living organisms that cannot be seen with the naked eye Pathogen- a microscopic organism that causes disease Aerobic microbes- must have oxygen Anaerobic microbes- grow without oxygen

5. PATHOGEN INFO Pathogen Type Growth factors Positive presence Disease example Methods of Destruction Bacteria Fungi Protozoa Rickettsiae (parasite) Viruses

6. 5 TYPES OF PATHOGENS 1)Bacteria- different types, classified by shape and arrangement: a)cocci (round)- Diplococci, Staphylococci, Streptococci b)bacilli (rod shaped) c)spirilla (spiral or corkscrew) * Can be treated with antibiotics

7. TYPES OR PATHOGENS (CONT.) 2)Fungi- ringworm, athlete’s foot, yeast infections Treated with anti- fungal medicines 3)Protozoa- animals found in contaminated water supplies can cause malaria, trichomoniasis, and amebic dysentery

8. PATHOGENS (CONT.) 4)Parasites- must live inside a host, can transmit disease between organisms 5)Viruses- smallest, difficult to destroy, use other cells to replicate, spread through blood and body fluids

9. FACTORS INFLUENCING MICROBIAL GROWTH 1) Temperature 2) pH 3) Food/nutrients 4) Moisture 5) Oxygen

10. METHODS THAT DESTROY MICROORGANISMS 1) Antiseptics- applied directly to the skin, not useful against all organisms Ex: Alcohol, betadine 2) Disinfectants- strong chemical to use on surfaces, not skin- kills most but not all, spores can survive Ex: Bleach and zephirin 3)Sterilization- kills microbes and spores * Ex: chemicals, radiation, gas, steam and pressure

11. INFECTION Infection -invasion of a host by disease- causing agents, their multiplication, and the reaction of host tissues to these organisms.

12. CHAIN OF INFECTION http://www.probloodborne.com/en/training_video/ how-bloodborne-pathogens-are-spread http://www.probloodborne.com/en/training_video/ how-bloodborne-pathogens-are-spread

13. INFECTION Viral infection vs. Bacterial infection

14. POSSIBLE SIGNS OF INFECTION Swelling Redness Warmth Fever Itching Burning Pain/tenderness Puss/sore/drainage