1. Rev 3.09 6-1 INTOXILYZER 5000EN THEORY/OPERATION

2. Rev 3.096-2 Infra- Red Light Absorption I nfra - Red light is a form of energy known as: ELECTROMAGNETIC RADIATION The Electromagnetic Scale Fast Slow cosmic rays gamma rays x-rays light Ultra- violet visibleinfraredmicr o wave radio wave

3. Rev 3.096-3 All things will absorb ELECTROMAGNETIC RADIATION in their own unique and consistent manner

4. Rev 3.096-4 THEREFORE All things will absorb INFRARED LIGHT in their own unique and consistent manner And will create their own unique and consistent absorption pattern

5. Rev 3.096-5 THE IR FINGERPRINT OF ETHANOL 3.47 Micron

6. Rev 3.09 6-6 SPECTROPHOTOMETRY The science of measuring the absorption of light or radiant energy to identify and quantify a substance

7. Rev 3.096-7 BASIC SPECTROPHOTOMETRY PRINCIPLES Less Concentrated/ Appears LighterMore Concentrated/ Appears Darker Incidental Radiation Emerging Radiation Incidental Radiation Emerging Radiation

8. Rev 3.09 6-8 SPECTROPHOTOMETER An instrument that is able to use light or radiant energy to identify and quantify a substance

9. Rev 3.09 6-9 SPECTROPHOTOMETER (INTOXILYZER 5000EN) An instrument that is able to use light (Infrared Light) or radiant energy to identify and quantify a substance (Ethanol).

10. Rev 3.09 6-10 SPETROPHOTOMETRIC RELATIONSHIPS 1. Measurements determine concentration. 2. Measurements are made relative to a standard (known) concentration. 3. Quantitative analysis is governed by the BEER- LAMBERT LAW.

11. Rev 3.09 6-11 BEER - LAMBERT LAW Absorption is directly proportional to concentration.

12. Rev 3.09 6-12 BEER - LAMBERT LAW (GRAPHIC REPRESENTATION) CONCENTRATION ABSORBANCEABSORBANCE

13. Rev 3.09 6-13 INTERACTION OF INFRARED ENERGY WITH THE ETHANOL MOLECULE H H H H H C C H C C OH OH H H H H

14. Rev 3.096-14 Light Source Sample Chamber LENS Filter the light striking the photo detector produces a level of energy labeled as X BREATH SAMPLE ANALYSIS STEP # 1 Photo Detector

15. Rev 3.096-15 Light Source Sample Chamber LENS Filter Photo Detector Breath Sample In Sample Out The light striking the detector after alcohol is introduced produces a different level of energy labeled as Y The light striking the photo detector produces a level of energy labeled as X STEP # 2 BREATH SAMPLE ANALYSIS

16. Rev 3.096-16 Infra-Red Theory If we establish the amount of IR light able to pass through a chamber with no alcohol present X Then establish the amount of IR light able to pass through a chamber with alcohol present Y Then X Y - = Alcohol Concentration

17. Rev 3.096-17 Light Source Sample Chamber LENS Filter Photo Detector BREATH SAMPLE ANALYSIS 3.80 - Reference 3.36, 3.40, 3.52 - Interferents 3.47 - Alcohol

18. Rev 3.096-18 ABSORPTION BAND OPTICAL FILTERS (TRANSMITTANCE IN PERCENT) REFERENCE 3.80 MICRONS ETHANOL 3.47 MICRONS TOLUENE 3.36 MICRONS ACETONE 3.40 MICRONS ACETALDEHYDE, ISOPROPANAL, METHANOL, ETHER, ETC 3.52 MICRONS WAVELENGTH IN MICRONS

19. Rev 3.09 6-19