1. Refining Margarine Crypthecodinium cohnii: A natural DHA Factory 20 % lipid content by weight which is 40 % DHA Facultative heterotroph, which does not perform photosynthesis Growth rate of 0.0375 h -1 on glucose substrate Uses glucose as a carbon source Uses dead yeast from ethanol bottoms as a nitrogen source Requires dissolved oxygen for optimal growth Grows in brackish (saline) water. Fermentation Travis Dallas Eric Graves Joaquin Martinez Chris McNinch Ramune O. Meskyte Charu Saini Mentors: Dan Rusinak, Jeffery Perl, John Micheli Lack of DHA is associated with poor or slower retinal development and brain development Helps infants gain weight faster Recommended 300 mg/day for pregnant or lactating women A lack of DHA is associated with Alzheimer’s disease Inhibits the growth of human colon carcinoma cells Increases the efficacy of chemotherapy Infant Health Adult Health C 22 H 32 O 2 INGREDIENTS: INTERESTERIFIED SOYBEAN OIL, WATER, ALGAE OIL, NATURAL SEA SALT, MONO/DIGLYCERIDES, NATURAL LECITHIN, SORBIC ACID, BETA-CAROTENE (VITAMIN A), ERGOCALCIFEROL (VITAMIN D), DIACETYL (FLAVORING) Docosahexaenoic Acid HO O Fermentation Controls 35,000 gallon Fermentor Height 50 feet Diameter 12 feet Total Project Capital Cost 37,328,232.77 USD Operating Costs 18,962,032.85 USD/Year  Raw Materials 6,031,280.00 USD/Year  Utilities3,532,157.83 USD/Year  Maintenance2,030,000.00 USD/Year  Operating Labor Costs2,828,000.00 USD/Year  Operating Charges 707,000.00 USD/Year  Plant Overhead2,429,000.00 USD/Year  G and A Costs1,404,594.96 USD/Year Total Product Sales 25,963,238.43 USD/Year Payback Period 5.7 Years Net Present Value 84,727,200.17 USD Internal Rate or Return 25.5% Cumulative Cashflow Cumulative Cash Flow Basis: 1% of Market Year of Plant Life Cumulative Cashflow Cumulative Cash Flow Basis: 2% in 6 Years Year of Plant Life Cumulative Cashflow Cumulative Cash Flow Basis: 2% of Market Year of Plant Life Expand to 2% Market Share in 6 Years Payback Period 7.13Years Net Present Value158,394,244.01USD Internal Rate or Return34.5% 2% Market Share from Start Payback Period 5.00Years Net Present Value198,627,853.09USD Internal Rate or Return34.5% Growth Phase Maintenance What is Sanitary Design? Preventing microbial growth and simply being clean How do you go about this? Physical separations of the different plants All equipment composed of stainless steel Margarine Plant composed of polished stainless steel Linear and horseshoe shaped plant design Clean in Place system (CIP) on the inside Spray cleaning foam on the outside Who cares? You do, and so do we. We do not want you getting sick from our product. Algae Oil Alpha Algae Co. 1928 SE Otis Rd. Cedar Rapids, IA 52401 Alpha Algae Co. Truck Engines Just Spread It On! Sooo Healthy! 0g Trans Fatty Acids 100% Vegan 100% Daily Omega-3 Fatty Acids per Serving Eat Premium Golden rather than me for your essential fatty acids. Butter your biscuits! We like it clean! You should get DHA from algae just like me. It is tons healthier for both of us. Algae Growth Cycle 0 5 10 15 20 25 30 020406080100 Time (Hours) Cell Density (g/L)