1. Collier Regional Science and Engineering Fair 2015-16

2. All projects MUST complete 1A, 1B, 3, Research Plan Abstract (official SSEF), Conclusion (after experimentation) Special Projects require additional forms 1C – Regulated Research Institution 2 – Qualified Scientist 4 – Human Subject and Informed Consent 5A – Vertebrate Animal 5B – Vertebrate Animal 6A – Potentially Hazardous Biological Agents 6B – Human and Vertebrate Animal Tissue 7 – Continuation Projects Form to be complete PRIOR to experimentation FORMS

3. Fillable Form – Have Students use this form Research Plan The research plan (and all other forms) can be accessed and downloaded from the CRSEF website. Or click the document to the right.CRSEF website

4. CRSEF RulesSSEF RulesISEF Rules Local rules supersede state, which supersedes ISEF Rules

5. Any experiment that involves vertebrate animals, potentially hazardous biological agents, or hazardous chemicals, devices or activities require that the SRC committee approves the research plan PRIOR to any experimentation. Any project that involves human subjects requires that the IRB committee approve the project PRIOR to any experimentation. Scientific Review Committee Institutional Review Board SRC VS IRB

6. Live, nonhuman vertebrate mammalian embryos or fetuses. Tadpoles Bird and reptile eggs within 72 hours of hatching. All other nonhuman vertebrates, including fish at hatching or birth. Research plan MUST address reasons WHY the vertebrate is the best choice as the subject of the project (see the 4 R’s p.11) Replace, Reduce, Refine, Respect Please send all vertebrate research plans to Mary Marshall before October 15 for review. Vertebrate Animals Special Projects

7. Bacteria, Viruses, Mold, Algae, Tissues BSL – 1 projects E.coli K-12, any microbial project in which the culture is NEVER opened. Double bagged, taped Petri dishes, sealed tubes. Detailed descriptions of collection, aseptic techniques and disposal methods for media and cultures. BSL – 2 projects No junior level student may conduct a BSL-2 project. (opening an actively growing culture or sub-culturing) Send ANY project that “grows or cultures” a microbial agent to Mary Marshall for review no later than October 15. PHBA’s Potential Hazardous Biological Agents Special Projects

8. Studies involving fresh/frozen tissue, blood or body fluids obtained from humans and/or vertebrates may contain microorganisms and have the potential of causing disease. Blood Muscle tissue Feathers plucked from living or non-living birds Exempt tissues include Fingernails, shed feathers, sterilized teeth, fossilized specimens, prepared fixed tissue, pasteurized milk, fresh or frozen meat products from the grocery, plant and non- primate established cell lines. Advice: if the project involves tissue of ANY kind, contact Mary Marshall to confirm exemption status. (send research plan) Tissues Special Projects

9. Any project that involves human participants must be approved by the IRB BEFORE any experimentation begins. Physical activities, ingestion of ANY substance, student designed invention testing by anyone other than the student, behavioral observations, surveys, etc. Some human projects may be exempt under CERTAIN conditions: Data record/review from pre-existing data bases that do not involve any human interactions. Behavior observations that do not in any way interact or affect the individuals being observed The researcher does not manipulate the environment The researcher does not record any personally identifiable data Be on the side of caution – submit ANY human project research plans to Mary Marshall before October 15 for clarification of exemption status. Human Projects Special Projects

10. For ANY project using a chemical (whether household or not, please consult the SDS (safety data sheet) for that chemical. If it has an NFPA rating of 1 or higher, the project will need SRC review PRIOR to any experimentation. Safety procedures are very important. Consult p. 18-20 in the ISEFrule book for more information regarding chemicals. Consider the “SPHERE OF INFLUENCE” – use the minimum concentration necessary and all disposal procedures MUST be included in the procedure. Weapons must be discharged at a certified range with a range officer. (SRC pre-approval required) Power tools may not be used by junior researchers. The procedure and safety precautions are very important in projects that require the use of power tools. Hazardous Chemicals/Devices/Activities Special Projects

11. SDS or MSDS sheets NFPA Rating

12. Vitamins Gasoline Paint thinner Alcohol Flammables Moth Balls Diesel Cleaners Provide an SDS sheet with the Risk Assessment Form 3 / check the NFPA rating Household Chemicals Safety precautions MUST be included in the procedure, not just on the risk assessment form. It is preferred that they be part of the procedure, not in a separate section. Sources must be reliable – not “my teacher” or “my parent”. Cite the SDS sheets in the bibliography.

13. Submit electronically to crsefsrc@gmail.comcrsefsrc@gmail.com Include student name, grade, school in subject line. Submit a DETAILED research plan with safety precautions, bibliography and disposal techniques (if required) Submit the required signed forms. Please indicate the desired email address for project return. Contact information: Mary Marshall (SRC/IRB Chair) crsefsrc@gmail.com 239-377-0431(office) or 239-307-9022 (cell) OCTOBER 15, 2015 - DEADLINE For Projects that Require Approval