1. Biotech Lab Safety Material Safety Data Sheets Hazards in the Lab Chemical Classes

2. Bell Work  Answer the following question in your lab notebook: Why do you think safety and safety training is important for laboratory work?

3. Objectives  Identify specific biological/biohazardous/ chemical materials. (1.7)  Students will locate the material safety data sheets for the facility, and identify appropriate internet MSDS resources.  Students will apply knowledge of material safety data sheets (MSDS) (1.3)

4. Key Terminology  Material Safety Data Sheet (MSDS)  Biohazard  Safety Equipment

5. Safety Equipment With your table group:  Define Safety Equipment on your whiteboard.  Be prepared to share your table’s definition. Based on our class definition:  Sort the Deck into “Safety” and “Non-Safety” groups.

6. Introduction  Science may be _______________, but can be done safely when hazards are ________________.  Everyone is ____________________: students, teachers, supervisors, and higher administrators.  All safety information brought to you today applies to all science disciplines.  Remember - each discipline has _____________ & ___________information and safety protocols.

7. Hazards in the Lab: Biological Controls  You will encounter biological materials throughout this course.  Wear proper __________ ___________ __________  Universal Precautions: Treat everything like it is infectious.  Disinfect your lab bench each day:  School provided disinfectant spray  Freshly prepared solution of 1 part household bleach and 10 parts water  70% _____________________ (____________)

8. Hazards in the Lab: Biological Hazards  What could be encountered?  ________________, fungi/mold  Injured student/ faculty  Human ___________sampling, ____________  (not common in high school labs)

9. Hazards in the Lab?  Where do you start?  Gather Standard Operating Procedures and other information for your facility  M______________ S______________ D______________ S______________

10. Material Safety Data Sheet  Here is the MSDS for Sodium chloride (NaCl)  What sections are found on an MSDS?  1. Paste the MSDS into your Lab Notebook  2. Highlight the sections listed on an MSDS  Section 1 = ?  Section 2 = ?  Etc.

11. Material Safety Data Sheets (MSDS)  Contain all safety and use information about a chemical.  _________________are required to provide them when chemicals are purchased.  MSDS are an important part of _________Hazard Communication Standard (1910.1200)  MSDS must be readily available to ______________.  Be sure to read all sections before using a chemical.

12. MSDS Activity Sheet  Using the MSDS Activity Sheet:  Think about the answers on the worksheet  Pair up, and SHARE with your neighbor what the answers would be….don’t write anything yet!

13. Where’s the MSDS?  Material Safety Data Sheets are required to be present in ANY facility that uses a chemical.  Where are ours?  Record the location in your lab notebook  You can even take a picture with your digital camera or phone if the teacher says yes, and paste that in your lab notebook

14. Find One!  Using the internet, or one of the MSDS Binders, locate an MSDS for a chemical used in our facility.  Some chemicals used in our facility are:  Copper Sulfate Pentahydrate  LB Agar  LB Broth  Ethidium Bromide  Ethanol  Sodium Hydroxide  Acetic Acid  TAE Buffer  Crystal Violet  Glucose http://www.flinnsci.com/search_MSDS.asp http://www.fishersci.com/http://www.fishersci.com/ Click MSDS Search

15. Thumbs Up-Thumbs Down  Was the MSDS binder in an easily accessible location?

16. Hazards in the Lab: Chemical Classes  Acids  Bases  Solvents  Flammables  Halogenated  Pyrophoric  Peroxide Forming  Toxic  Oxidizers  Chemicals are segregated according to class:  Information about Toxicity, oxidation, etc are identified on the MSDS

17. Chemical Classes: _____________  Def: The pH range of acids is from 0.1- 6.9.  Examples: hydrochloric acid (HCl), acetic acid, nitric acid, phenol, sulfuric acid  Storage Precautions:  Store in large bottles of acids on low shelf or in acid cabinets.  Segregate acids from bases.  Have ____________ _______ with neutralizers and absorbent.

18. Proper Storage for Acids:

19. Inside Look at Acid Storage:

20. Improper Acid Storage Rust

21. Chemical Classes: _____________  Def: The pH range of basic solutions is from _________ -- _________  Examples: sodium hydroxide (NaOH), ammonium hydroxide  Storage Precautions:  Segregate bases from acids  Have a spill kit with neutralizers and absorbent.

22. Chemical Classes: Flammables  Def: Has a flash point of 60.5°C (141°F) or lower  Examples: acetone, ethanol, methanol, isopropyl alcohol  Storage Precautions:  Store in approved safety _______or ____________  Segregate from oxidizing acids and oxidizers  Keep away from any source of _____________: flames, localized heat, or sparks.  Store highly volatile flammable liquids in a specially equipped refrigerator.

23. Example of Proper Storage: Flammables

24. Chemical Classes: Pyrophoric  Def: A material which ignites spontaneously upon exposure to air (or oxygen).  Examples: Powders: calcium, zinc, lead, iron, nickel  Storage Precautions: Store ___________in a cool, dry place

25. Chemical Classes: Peroxide-Forming Chemicals  Def: Organic compounds that spontaneously form peroxides by a free-radical reaction with molecular oxygen in a process of auto-oxidation.  Examples: ethyl ether, isopropyl ether, acetaldehyde  Storage Precautions:  Store in airtight containers in dark, cool, dry place.  Label containers with receiving, opening, and disposal dates.  Dispose of peroxide-forming chemicals before expected date of first peroxide formation.  Test for presence of peroxides periodically.

26. Chemical Classes: __________  Def: Dangerous or extremely dangerous to health and life when inhaled, swallowed, or absorbed by skin contact.  Examples: phenol, arsenic, chloroform, nitric acid, sulfuric acid, hydrogen peroxide, hydrochloric acid  Storage Precautions:  Store according to hazardous nature of chemical, using appropriate security when necessary. Turn to your partner – Where would you find the information about how to store the chemical?

27. Find The Safety Equipment!  Take a quick tour of your facility!  Locate and record in your lab notebook the location(s) of:  Hazards in the Lab (biohazards or chemical)  Safety Equipment  Chemical Storage Locations (look for labeled, specialty cabinets and standard cabinets with chemicals)

28. Chemical Hazards Key issue in use, storage, and disposal is: ___________________ ___________________!! Thumbs Up-Thumbs Down Is chemical compatibility information on an MSDS?

29. Chemical Compatibility Chart

30. Closure  Why are material safety data sheets required in a facility?  Take one minute to THINK, then WRITE for one minute about why facilities require MSDS, and why YOU as a technician should read them often.

31. Homework 1.Complete the MSDS Activity Sheet using the MSDS you found. If an electronic version is used, you DO NOT need to print it, but you do have to record your source website. 2.Identify at least 3 hazards in your facility. Describe them, and determine ways to prevent or minimize them. Due tomorrow at the beginning of class.

32. References  Roy, Ken; Safe Science Series http://www.nsela.org/index.php?option=com_content&view=category&id =71&Itemid=79 June 09, 2011. http://www.nsela.org/index.php?option=com_content&view=category&id =71&Itemid=79  Lab Safety  http://cfo.asu.edu/ehs-labsafety, June 9, 2011 http://cfo.asu.edu/ehs-labsafety  http://cfo.asu.edu/ehs-labsafety-references, June 9, 2011. http://cfo.asu.edu/ehs-labsafety-references  Chemical Safety  “Chemical Safety for Teachers and Their Supervisors- Grades 7-12”, A publication of the American Chemical Society and the ACS Board-Council Committee on Chemical Safety. Send email to oss@acs.org for a copy.oss@acs.org  MSDS  http://www.flinnsci.com/search_MSDS.asp June 9, 2011 http://www.flinnsci.com/search_MSDS.asp  http://www.fishersci.com/ Click MSDS Search June 10, 2011 http://www.fishersci.com/