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Published byBeverly McDaniel Modified over 8 years ago
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TaqMan Gene Expression Arrays for Accurate Quantification of Toxicity Targets
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Agenda Test Procedure Specifications Validation Results
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Test Procedure RNA isolation cDNA generation (may be skipped with one-step RT-PCR) Card loading and sealing Thermal cycling Data Analysis
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Dynamic Range in the Cytokine Card
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Average plus 6 Stdev is Fold Discrimination Calculate Average of 40 RQ values Normalize RQ values to positive fold Calculate RelQuant Values (20 for each sample--40 total ) Calculate dCt CalibratorCalculate dCT sample 1Calculate dCt sample 2 3 cytokine cards with 20 plasmid targets Fold-Discrimination in the Cytokine Card
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DNAx: Cytokine Gene Expression and PBMC Activation
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Source Precision Medicine: Cytokine Gene Expression and PBMC Activation
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Phase 1 Molecular Toxicology: Cytokine Gene Expression in Liver Cell Cultures
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Phase 1 Molecular Toxicology: MMC Incubations of Liver Cells in the Human Toxicology Card
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Phase 1 Molecular Toxicology: MMS Incubations of Liver Cells in the Human Toxicology Card
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Phase 1 Molecular Toxicology: Carnustine Incubations of Liver Cells in the Human Toxicology Card
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Phase 1 Molecular Toxicology: Cytokine Gene Expression in RNA Isolated from Blood Cells
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Phase 1 Molecular Toxicology: Toxicology Gene Expression in RNA Isolated from Blood Cells
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Phase 1 Molecular Toxicology: Cytokine Gene Expression in RNA Isolated from Lymphocyte Cultures
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Phase 1 Molecular Toxicology: Toxicology Gene Expression in RNA Isolated from Lymphocyte Cultures
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