1. What we’ll cover: 1.Tissues of the skeletal system 2.Functions of the skeletal system 3.Cartilages of the skeletal system 4.Bone development 5.Ossification 6.Cells of the skeletal system 7.Types of bone 8.Gross anatomy of bones 9.Classification of bones 10.Bone features 11.Bone injuries 12.Aging bones

2. Skeletal Tissues JACKI HOUGHTON, DC Contains cartilages, ligaments, blood vessels, nerves and connective tissues Provides support/framework for the body Stores mineral, lipids (in yellow bone marrow) Blood cell production Provides levers for muscles to produce actions Contains calcium and phosphate ions

3. Define Cartilage – remember your connective tissues? What should I know about cartilage? 1.What are the types of cartilages? 2.Where would I find each of these types of cartilages? 3.What are the functional properties of cartilage as a tissue?

4. Cartilage Embryo –More prevalent in the embryo than in adult –Skeleton is initially mostly cartilage –Bone replaces cartilage in fetal and childhood periods –3 types: hyaline, elastic and fibrocartilage So what is cartilage? It is a connective tissue which has differing properties, depending on it’s function. Hyaline cartilage lines the ends of bones and cushions them. The hyaline wears better than bone. Elastic cartilage is still but will bend and return to it’s original shape. Fibrocartilage has great tensile strength and can absorb shock.

5. Cartilages 3 types –Fibrocartilage –Elastic cartilage –Hyaline cartilage

8. Location of cartilage in adults External ear - elastic Nose - hyaline “Articular” – covering the ends of most bones and movable joints - hyaline “Costal” – connecting ribs to sternum - hyaline Larynx - voice box -elastic

9. Epiglottis – flap keeping food out of lungs - elastic Cartilaginous rings holding open the air tubes of the respiratory system (trachea and bronchi) hyaline Intervertebral discs - fibrocartilage Pubic symphysis - fibrocartilage Articular discs such as meniscus in knee joint - fibrocartilage

10. Functions of the Skeletal System –Support against gravity –Protection of soft internal organs –Movement (Leverage) –Storage Minerals (calcium, phosphorous) – within the matrix of bone tissue Energy reserve (adipose) – within the yellow marrow of long bones –Blood cell production – within red marrow of spongy bone tissue

11. Chemical composition of bones Cells, matrix of collagen fibers and ground substance (organic: 35%) –Contribute to the flexibility and tensile strength Mineral crystals (inorganic: 65%) –Primarily calcium phosphate –Lie in and around the collagen fibrils in extracellular matrix –Contribute to bone hardness Small amount of water

12. Bone development Osteogenesis: “formation of bone” –From osteoblasts –Bone tissue first appears in week 8 (embryo) Ossification: “to turn into bone” –Intramembranous ossification (also called “dermal” since occurs deep in dermis): forms directly from mesenchyme (not modeled first in cartilage) Most skull bones except a few at base Clavicles (collar bones) Sesamoid bones (like the patella) –Endochondral ossification: modeled in hyaline cartilage then replaced by bone tissue All the rest of the bones

13. Remember the three germ tissues… 1.Ectoderm - epithelial 2.Endoderm - epithelial 3.Mesoderm is a mesenchyme tissue –Mesenchyme cells are star shaped and do not attach to one another, therefore migrate freely –From the last slide: Intramembranous ossification: forms directly from mesenchyme (not modeled first in cartilage) Most skull bones except a few at base Clavicles (collar bones) Sesamoid bones (like the patella)

14. Intramembranous ossification 1.ossification centers appear in the fibrous connective tissue membrane. Certain mesenchymal cells cluster and differentiate into osteoblasts, forming an ossification center. 2.Bone matrix (osteoid) is secreted within the fibrous membrane and calcifies. Osteoblasts begin to secrete osteoid which is calcified within a few days. Trapped osteoblasts become osteocytes. 3.Woven bone and periosteum form. Blood vessels perforate the growing bone in a random manner resulting in a network instead of lamellae and forms trabeculae called woven bone. 4.Lamellar bone replaces woven bone, just deep to the periosteum. Red marrow appears. Eventually a plate of compact bone forms on the cortex of the flat bones. (osteoid is the organic part)

15. Endochondral ossification Modeled in hyaline cartilage, called cartilage model Gradually replaced by bone: begins late in second month of development Perichondrium is invaded by vessels and becomes periosteum Osteoblasts in periosteum lay down collar of bone around diaphysis Calcification in center of diaphysis Primary ossification centers Secondary ossification in epiphyses Epiphyseal growth plates close at end of adolescence –Diaphysis and epiphysis fuse –No more bone lengthening See next slide

16. Endochondral ossification Stages 1-3 during fetal week 9 through 9 th month Stage 4 is just before birth Stage 5 is process of long bone growth during childhood & adolescence

18. Epiphyseal growth plates in child, left, and lines in adult, right (see arrows)

19. Factors regulating bone growth Vitamin D: increases calcium from gut Parathyroid hormone (PTH): increases blood calcium (some of this comes out of bone) Calcitonin: decreases blood calcium (opposes PTH) Growth hormone & thyroid hormone: modulate bone growth Sex hormones: growth spurt at adolescense and closure of epiphyses

20. Bone Composition Cells and matrix Matrix –Calcium phosphate 2/3, collagen fibers 1/3 –Collagen forms a lattice frame to hold salts Cells Osteocytes – mature bone cells which create and maintain a structure  ”osteon” Osteoblast – produces new osteocytes Osteoclasts – destroy worn out bone cells – are actually derived from blood cells (ref:http://edrv.endojournals.org/content/32/1/31.full) Osteoprogenitor Cells  from mesenchyme for bone repair

21. Compact bone Osteons: pillars (functional unit) Lamellae: concentric tubes Haversian canals Osteocytes

22. Spongy bone No osteons Layers of lamellae and osteocytes Seem to align along stress lines - traebeculae

23. Nutrients diffuse from vessels in central canal Alternating direction of collagen fibers increases resistance to twisting forces Isolated osteon: canaliculi

25. Long bones Tubular diaphysis or shaft Epiphyses at the ends: covered with “articular” (=joint) cartilage Epiphyseal line in adults – the epiphyseal plate usually closes at 20 years old –Kids: epiphyseal growth plate (disc of hyaline cartilage that grows to lengthen the bone) Blood vessels –Nutrient arteries and veins through nutrient foramen

26. Classification of bones by shape Long bones Short bones Flat bones Irregular bones Pneumatized bones Sesamoid bones (Short bones include sesmoid bones)

27. Gross anatomy of bones Compact bone Spongy (trabecular) bone Blood vessels Medullary cavity Membranes –Periosteum –Endosteum

28. Know these!

29. Flat bones Spongy bone is called diploe when its in flat bones –Have bone marrow but no marrow cavity

34. Bone markings reflect the stresses

35. Periosteum Periosteal Bud - A vascular connective tissue bud from the perichondrium that enters the cartilage of a developing long bone and contributes to the formation of a center for ossification.perichondriumcartilageossification Connective tissue membrane Covers entire outer surface of bone except at epiphyses Two sublayers –1. Outer fibrous layer of dense irregular connective tissue –2. Inner (deep) cellular osteogenic layer on the compact bone containing osteoprogenitor cells (stem cells that give rise to osteoblasts) Osteoblasts: bone depositing cells Also osteoclasts: bone destroying cells (from the white blood cell line) Secured to bone by perforating fibers (Sharpey’s fibers) Endosteum Covers the internal bone surfaces Is also osteogenic

36. Bone markings *Bone pain is called ostealgia* Projections that are the attachments sites for muscles and ligaments Surfaces that form joints Depressions and openings Learn them using: Marieb lab book p 101, Table 8.1, Bone Markings or Martini p 128, Table 5.1, Common Bone Marking Terminology (next slide)

37. Bone remodeling Osteoclasts –Bone resorption Osteoblasts –Bone deposition Triggers –Hormonal: parathyroid hormone –Mechanical stress Osteocytes are transformed osteoblasts

38. Terms (examples) chondro refers to cartilage –chondrocyte –endochondral –perichondrium osteo refers to bone –osteogenesis –osteocyte –periostium blast refers to precursor cell or one that produces something –osteoblast cyte refers to cell –osteocyte

39. Repair of bone fractures (breaks) Simple and compound fractures Closed and open reduction