1. The National Children’s Study Biospecimen Collections Mother – blood, urine, hair, nails, saliva, vaginal swab, breast milk Father – blood, urine, hair, nails, saliva Child – placenta (sections), cord blood, cord (section), meconium, blood, urine, hair, nails, saliva All biospecimens are aliquotted and stored There is no ‘real-time’ analysis of biospecimens

2. The National Children’s Study Chemical Exposures classified by “Domain of Exposure,” sub-classified by “Specific Measure” – Allergens (pets, dust, mold) – Carbonyls (formaldehyde, acrolein) – Metals (Pb, Hg, Cd, Mn, Uranium, As) – Gaseous Air Pollutants (ozone, SO2) – Pesticides (carbamates, pyrethroids, atrazine) – VOCs (benzene, chloroform) – ETS – Nitrates – Halogenated Phenols, & more

3. The National Children’s Study Chemical Exposure Measures divided into Household & Community samples Related Data Collection – Biological (blood, hair, nails, urine, breast milk) – Questionnaires – Observation (home, yard, neighborhood, agricultural area)

4. The National Children’s Study How will Birth Defects be identified? 1 st Trimester U/S Placenta photos, wt & dimensions, blocks &/or specimens Dysmorphology Assessment Instrument exam after birth while still in hospital PE at 6 & 12 months NUMEROUS physical measurements of pregnant Mo & child throughout

5. The National Children’s Study Data Collection related to Glucose Metabolism HbA1C @ pre-pregnancy & 1 st trimester Glucose, insulin, IGF, & IGF-BP3 @ 3 rd trimester