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Finding and Understanding Research Protocols Angelina Hoefle The University of Texas San Antonio MBRS-RISE & MARC-U*STAR Programs 08/2006.

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Presentation on theme: "Finding and Understanding Research Protocols Angelina Hoefle The University of Texas San Antonio MBRS-RISE & MARC-U*STAR Programs 08/2006."— Presentation transcript:

1 Finding and Understanding Research Protocols Angelina Hoefle The University of Texas San Antonio MBRS-RISE & MARC-U*STAR Programs 08/2006

2 What is a protocol? A detailed plan of a scientific or medical experiment, treatment, or procedure (Merriam Webster Online) A detailed plan of a scientific or medical experiment, treatment, or procedure (Merriam Webster Online) Indicates specific conditions, such as weight, volume, concentration, temperature, and time Indicates specific conditions, such as weight, volume, concentration, temperature, and time Similar to a recipe Similar to a recipe

3 Understanding the theory behind it More efficient experiments More efficient experiments Makes troubleshooting easier Makes troubleshooting easier Helps you optimize conditions to test a different idea/set of samples Helps you optimize conditions to test a different idea/set of samples

4 Where can you find protocol books? Current Protocols in Molecular Biology - reference section of John Peace Library (QH506.C87 1993) Current Protocols in Molecular Biology - reference section of John Peace Library (QH506.C87 1993) Other Current Protocols titles online (Cell Bio, Immuno, Neuro, Pharmacology, Protein Science) – accessible through UTHSC computers Other Current Protocols titles online (Cell Bio, Immuno, Neuro, Pharmacology, Protein Science) – accessible through UTHSC computers

5 Where can you find protocols online? www.protocol-online.org/ www.protocol-online.org/www.protocol-online.org/ The most comprehensive free online protocol website. The most comprehensive free online protocol website. Type the technique name + “theory,” “introduction,” or “background” to get more information Type the technique name + “theory,” “introduction,” or “background” to get more information www.bioexplorer.net/Methods and Protocols/ www.bioexplorer.net/Methods and Protocols/www.bioexplorer.net/Methods and Protocols/www.bioexplorer.net/Methods and Protocols/ www.cellbio.com/protocols.html www.cellbio.com/protocols.htmlwww.cellbio.com/protocols.html

6 Where else can you find protocols online? UTSA JPL netLibrary UTSA JPL netLibrary www.lib.utsa.edu www.lib.utsa.edu www.lib.utsa.edu Click Databases A-Z Click Databases A-Z Click “n” – find and click on netLibrary (or go directly to http://www.netlibrary.com.libweb.lib.utsa.edu/ Search/BasicSearch.aspx) Click “n” – find and click on netLibrary (or go directly to http://www.netlibrary.com.libweb.lib.utsa.edu/ Search/BasicSearch.aspx) http://www.netlibrary.com.libweb.lib.utsa.edu/ Search/BasicSearch.aspx http://www.netlibrary.com.libweb.lib.utsa.edu/ Search/BasicSearch.aspx Type the technique name and “method” Type the technique name and “method” e.g. “immunohistochemistry method” e.g. “immunohistochemistry method”

7 netLibrary Books of Interest to You Molecular Biology Problem Solver – A Laboratory Guide by Gerstein, Alan S. Molecular Biology Problem Solver – A Laboratory Guide by Gerstein, Alan S. Practical HPLC Method Development by Snyder, et. al. Practical HPLC Method Development by Snyder, et. al. Microscopy, Immunohistochemistry, and Antigen Retrieval Methods: For Light and Electron Microscopy by Hayat, M.A. Microscopy, Immunohistochemistry, and Antigen Retrieval Methods: For Light and Electron Microscopy by Hayat, M.A. Electron Microscopy Methods and Protocols, by Hajibagheri, M.A. Electron Microscopy Methods and Protocols, by Hajibagheri, M.A.

8 Other Online Resources Online Books (for background information) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi? db=Books Online Books (for background information) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi? db=Books http://www.ncbi.nlm.nih.gov/entrez/query.fcgi? db=Books http://www.ncbi.nlm.nih.gov/entrez/query.fcgi? db=Books Helpful Tools http://www.mgb.pitt.edu/moleculartoolbox.htm Helpful Tools http://www.mgb.pitt.edu/moleculartoolbox.htm http://www.mgb.pitt.edu/moleculartoolbox.htm Computational Resources for molecular biology http://molbio.info.nih.gov/molbio/ Computational Resources for molecular biology http://molbio.info.nih.gov/molbio/http://molbio.info.nih.gov/molbio/ Collections of books (must enter research field) http://crs.nsdl.org/collection/ Collections of books (must enter research field) http://crs.nsdl.org/collection/http://crs.nsdl.org/collection/

9 Acknowledgements Dr. Gail P. Taylor Dr. Gail P. Taylor All of you!!! All of you!!!

10 Questions???

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