1. Dragline in operation along FL 62 nr Ft Green Springs Design of Water Quality Monitoring

4. How do you find trends in water quality, without historic data?

5. Assessment of Effects of Differing Watershed Land Uses on Water Quality based on Comparisons of Six Stream/River Systems: 1.St. Mary’s River- Control 2.Myakka River-Large Agricultural Impact 3.Horse Creek – Limited Impacts of Agriculture & Mining 4.Payne Creek- Large Phosphate Mining Impact 5.Peace River at Arcadia- Combined Agricultural & Mining Impacts 6.S. Prong Alafia River- 60-70% Mined Basin

6. St Mary’s River- control No Trends in Water Quality over Time

7. Myakka River- Agricultural Pollution: Increasing sp. cond. & [K]

8. Specific Conductance- Myakka River

9. Potassium- Myakka River

11. 95th 75th 50th 25th 5th

12. Six Site Comparison of Mean Data for Seven Water Quality Parameters- Mined Watersheds Elevated in: 1. Specific Conductance 6. [F] 2. [Na] 7. [TP] 3. [Ca] 4. [Mg] 5. [SO4]

13. Specific Conductance

14. Sodium

15. Calcium

16. Magnesium

17. Sulfate

18. Fluoride

19. Total Phosphorus

20. Will such changes in water quality affect the fauna?

21. Bishop Harbor Algal Bloom in Response to Piney Pt. Effluent

22. Effluent Treatment & Discharge

25. Water Quality in Reclaimed Wetland Streams 1.Hall’s Branch 2.Jamerson Jr. 3.Lizard Branch 4.Dogleg 5.Pickle 6.Tadpole

27. The Normal Progression by Levels of Approximation in Field Toxicity Testing: 1.TIER 1- Is there toxicity by standard lab. bioassays? 2.TIER 2- Is there toxicity for native species in situ? Are species “missing” that should be present? 3.TIER 3- What is the toxicity due to? 4.TIER 4- What is the source of the toxin(s)?

28. Chronic Toxicity in Reclaimed Wetlands (Charlotte Cty. Data: 10/25/03; 3/2/04) Crustacean FETAX Site pH Sp. Cond. Repr. Malfor. Growth Jam. 6.9 106,148 + + Lizard 7.6 290,296 + + Pickle 6.8 395,486 + + Halls 6.8 340,483 + Dog 7.5 309,328 + Tadpole 7.2 220,403 +

29. WATER CHEMISTRY OF RECLAIMED STREAMS USED FOR BIOASSAYS BY CHARLOTTE COUNTY, Oct. 2003; March 2004 Parameter Dog Hall’s Jamerson Lizard Pickle Tadpol e pH 7.8 6.7 6.5 8.1 6.7 6.9 7.1 6.9 7.1 7.1 6.9 7.5 Sp Cond, uS/cm 328 483 148 290 486 403 309 340 106 296 395 220 Oxygen, mg/l 6.8 1.5 7.0 12.6 0.8 2.8 8.6 2.1 8.0 7.1 1.2 2.1 Iron, ug/l 325 117,000 583 547 4230 11,300 212 4025 195 352 786 1100 Manganese,ug/l 41 1880 136 71 127 166 22 392 21 30 72 20 Aluminum, ug/l 160 226 391 445 107 669 132 35 101 45 <5 95

30. Acute (A) and Chronic (C) Toxicity in Horse Creek Tributaries (IMC- Marinco data;1/21/04) Site pH Sp. Cond. Ceriodaph. Shiner Fathead 8W 4.4 74 A,C A C 1W 5.1 122 C C 2E 5.6 88 C C 6W 5.9 97 C C Piez. 7.8 266

31. HCSP Needs Changes Water quality - re-set trigger levels; add sampling sites above and below points of discharge and increase number of samples Bio-monitoring - use full 3 tier system of bioassays on field observations, lab tests and in-situ enclosures

32. HCSP Needs Changes The Bottom Line - Design rigorous plan for bio-monitoring prior to agreement - Convene outside panel free from conflicts of interest to design plan and oversee implementation

33. A Physical Analogy for the Cumulative Adverse Ecological Impacts of Mining