1. DNA Fingerprinting Daniel Lim Lin Siqi Si Hongbo Wang Chengxu

2. Guideline Definition of DNA Fingerprinting The techniques and methods When to use Problem with it Interesting Cases

3. Definition The process of comparing the nucleotide sequences of different DNA samples to find out if the samples are from the same individual or not. It was invented by Sir Alec Jeffreys at the University of Leicester in 1985.

4. Techniques and Methods RFLP Analysis STR Analysis

5. RFLP Analysis Performing a Southern Blot Making a Radioactive Probe Creating a Hybridization Reaction VNTRs (Variable Number Tandem Repeats)

6. Performing a Southern Blot http://protist.biology.washington.edu/fingerprint/blot.gif

7. Making a Radioactive Probe

8. Creating a Hybridization Reaction

9. VNTRs

10. STR Analysis Short Tandem Repeats PCR Amplication Electrophoresis

11. When to use it? Forensic evidence --- Criminal Identification Parenthood disputes --- Paternity and Maternity Establishment

12. Problem with it Generate a high probability Problems with this: 1.Population genetics 2.Technical difficulties

13. Interesting Cases In the 1920s, Anna Anderson claimed that she was Princess Anastasia Romanov of Russia; in the 1980s after her death, samples of her tissue that had been stored at a Charlottesville, Virginia hospital following a medical procedure were tested using DNA fingerprinting and showed that she bore no relation to the Romanovs.

14. Interesting Cases In 1987, British baker Colin Pitchfork was the first criminal caught using DNA fingerprinting in Leicester, the city where it was first discovered

15. Interesting Cases In 1994, Royal Canadian Mounted Police (RCMP) detectives successfully tested hairs from a cat known as “Snowball”, and used the test to link a man to the murder of his wife, thus marking for the first time in forensic history the use of non-human DNA to identify a criminal.

16. Q & A

17. Bibliography Campbell, N, A. Reece, J, B. Biology, Seventh Edition, 2005, Pearson. Audesirk, T. Audesirk, G. Byers, B, E. Biology: Life on Earth, Seventh Edition, 2005, Pearson. http://www.cartoonstock.com/ http://en.wikipedia.org/wiki/Genetic_fingerprintin g http://protist.biology.washington.edu/fingerprint