1. Cell Structure Analysis of the functional inter- relationships of cell structures

2. Cell Biology: the study of cells – the structural and function units of all living organisms morphologically cells vary in shape and size bacteria red blood cells white blood cells platelets nerve cell human sperm cells cancer cells (myeloma) portion of skeletal muscle cells cells also vary physiologically 1:2

3. Cell Theory 1.Cells are the basic unit of life 2.All organisms are made of one or more cells 3.Cells come from pre-existing cells

4. Cells Tissues Organs 1:3

5. Nucleus and Nucleoid a nucleoid (which consists of the organisms DNA) is NOT enclosed in a membrane = prokaryotic cell eukaryotic cell = DNA contained within a membrane 1:5

6. Eukaryotic Cells Bounded by cell membrane Cytoplasm inside cells composed of aqueous solution, organelles and molecules Various fibres compose a cytoskeleton to give shape and structure

7. Nucleus Contains genetic material which controls/directs the activities of the cell function: “brain” of cell, i.e. determines metabolism, growth, differentiation, & reproduction of cell Nucleus enclosed in a nuclear envelope Within nuclear envelope is chromosome/chromatin, nucleoplasm and nucleoli Chromosomes/chromatin are long strands of DNA wrapped around protein molecules which make up genes (units of heredity) nucleoplasm is a highly viscous liquid Nucleoli is the site where rRNA is assembled (rRNA then travels to the cytoplasm for protein syntheis)

8. DNA packed in Chromosomes

9. Chromatin vs. Chromosome

10. Ribosome consist of 2 non– identical subunits formed from rRNA & proteins function: protein synthesis found attached to endoplasmic reticulum or free in cytoplasm polysome: group of ribosomes simultaneously synthesizing same protein

11. Membranous Canals Includes endoplasmic reticulum, golgi apparatus, vacuoles and lysosomes

12. Endoplasmic Reticulum Series of membranous canals exist in cytoplasm Can extend from nuclear envelope to cell membrane rough endoplasmic reticulum: –ribosomes attached to surface –function: synthesize proteins for secretion NOTE: unlike free ribosomes that synthesize proteins for use within cell smooth endoplasmic reticulum: –no ribosomes attached to surface –functions: 1.synthesis of lipids, e.g. cell membrane & hormones 2.metabolism of carbohydrates, e.g. synthesis of glycogen 3.detoxify drugs & wastes in cell, e.g. alcohol

13. Golgi Apparatus resembles flattened stack of hollow tubes, i.e. similar to smooth endoplasmic reticulum, but closer to cell membrane function: sorting, modification (assembly), & packaging of substances for storage or secretion e.g. produces glycolipids by joining carbohydrates to lipids receives vesicles from endoplasmic reticulum that contain newly synthesized proteins/lipids proteins/lipids sorted, modified, & packaged into new vesicles that bud off vesicles transported within cell or moved to cell membrane for export by exocytosis

14. Vacuoles large, membrane enclosed sac vesicle: small vacuole functions: storage or transport formed by: 1. budding off (pinching off) from Golgi apparatus 2. endocytosis of cell membrane 3. extension of endoplasmic reticulum membrane

15. Lysosomes vesicle formed by Golgi apparatus, which contains hydrolytic enzymes functions: 1. cellular digestion of food particles, i.e. fuses with food vacuoles & enzymatically digests proteins, carbohydrates, & lipids 2. disposal of damaged cell organelles, e.g. mitochondria 3. breakdown of whole cell, i.e. releases contents into cell cytoplasm

16. Energy related organelles

17. Mitochondria function: cellular respiration (aerobic metabolism), i.e. converts glucose (carbohydrates) to energy (ATP) chemical reaction: C 6 H 12 O 6 + 6O 2 6CO 2 + 6H 2 O + energy (ATP) cristae (singular: crista): shelf–like structures formed from folded inner membrane with enzymes on surface for cellular respiration –Provides lots of surface area for cell. resp. reaction. matrix: solution surrounded by cristae contains DNA

18. Choloroplast function: photosynthesis in plant cells, i.e. converts sunlight energy to carbohydrates Made up of membranous stacks called grana, which contain chlorophyll pigments chlorophyll: chemical that absorbs sunlight energy & allows photosynthesis to occur

19. Basic Photosynthesis Works hand in hand with cellular respiration

20. Organelles made up of Membranes: Nuclear envelope Endoplasmic reticulum Golgi apparatus Lysosomes Vacuoles Vesicles Cell membrane Mitochondria Chloroplast Note: Membranes are composed of phospholipids

21. Cell Shape

22. Other Organelles Cell wall: –rigid cellular layer that surrounds the cell membrane in plant cells –Provides protection, shape, and support for the cell –Composed of cellulose Cytoskeleton –network of protein fibres –functions: maintain cell shape, anchor organelles, & involved in motility

23. Characteristics of Living Things Living things are made of cells. Living things obtain and use energy. Living things grow, reproduce and repair. Living things produce waste. Living things respond to their environment. Living things have a life span.

24. By the end of this section you should be able to Prokaryotic cell verses a eukaryotic cell Describe the following cell structures and their functions –Cell membrane –Cell wall –Chloroplast –Cytoskeleton –Cytoplasm –Golgi bodies –Lysosomes –Mitochondria – including cristae and matrix –Nucleus - including nuclear pore, nucleolus, chromatin, nuclear envelope, and chromosomes –Ribosomes (and polysomes) –Smooth and rough endoplasmic reticulum –Vacuoles –Vesicles State the balanced chemical equation for cellular respiration and photosynthesis Describe how the following organelles function to compartmentalize the cell and more materials through it: –Rough ER and smooth ER –Vesicles –Golgi bodies –Cell membrane Identify cell structures depicted in diagrams and electron micrographs