1. Microbial Growth & Control of Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements for Growth Chemical Requirements for Growth Growth of Bacterial Cultures Control of Growth Miscellaneous FINAL ROUND

2. Topic 1: Physical Requirements $100 Question How is a Psychrophile different from a Psychrotroph? BACK TO GAME ANSWER

3. Topic 1: Physical Requirements $100 Answer How is a Psychrophile different from a Psychrotroph? A Psychrophile is a microbe that can grow at   C, grows optimally at 15  C, and usually won’t grow at 25  C. A Psychrotroph is a microbe that can grow at    C, grows optimally at 20   -30  C, and usually won’t grow above 40  C. BACK TO GAME

4. Topic 1: Physical Requirements $200 Question Which one of the following is most likely to cause food spoilage in your refrigerator? a. Psychrophile b. Thermophile c. Psychrotroph d. Mesophile BACK TO GAME ANSWER

5. Topic 1: Physical Requirements $200 Answer Which one of the following is most likely to cause food spoilage in your refrigerator? a. Psychrophile b. Thermophile c. Psychrotroph (correct answer) d. Mesophile BACK TO GAME

6. Topic 1: Physical Requirements $300 Question What type of organism is Listeria monocytogenes? a. Psychrophile b. Thermophile c. Psychrotroph d. Mesophile BACK TO GAME ANSWER

7. Topic 1: Physical Requirements $300 Answer What type of organism is Listeria monocytogenes? a. Psychrophile b. Thermophile c. Psychrotroph (correct answer) d. Mesophile BACK TO GAME

8. Topic 1: Physical Requirements $400 Question What happens to most cells when they are exposed to a salt water solution? A) Nothing B) The cell swells and may undergo osmotic lysis C) The cell immediately divides D) The cell undergoes plasmolysis and shrinks BACK TO GAME ANSWER

9. Topic 1: Physical Requirements $400 Answer What happens to most cells when they are exposed to a salt water solution? A) Nothing B) The cell swells and may undergo osmotic lysis C) The cell immediately divides D) The cell undergoes plasmolysis and shrinks (correct answer) BACK TO GAME

10. Topic 1: Physical Requirements $500 Question Where might a hyperthermophile live? a. At the ocean floor b. In a human body c. In acid d. In salt water BACK TO GAME ANSWER

11. Topic 1: Physical Requirements $500 Answer Where might a hyperthermophile live? a. At the ocean floor (correct answer) b. In a human body c. In acid d. In salt water BACK TO GAME

12. Topic 2: Chemical Requirements $100 Question Chemoautotrophs and photoautotrophs derive their carbon from a. Carbon dioxide b. Proteins c. Lipids d. Carbohydrates BACK TO GAME ANSWER

13. Topic 2: Chemical Requirements $100 Answer Chemoautotrophs and photoautotrophs derive their carbon from a. Carbon dioxide (correct answer) b. Proteins c. Lipids d. Carbohydrates BACK TO GAME

14. Topic 2: Chemical Requirements $200 Question Microbial metabolism is responsible for biogeochemical cycles. A) True B) False BACK TO GAME ANSWER

15. Topic 2: Chemical Requirements $200 Answer Microbial metabolism is responsible for biogeochemical cycles. A) True (correct answer) B) False BACK TO GAME

16. Topic 2: Chemical Requirements $300 Question In which of the above tubes are Facultative Anaerobes growing? BACK TO GAME ANSWER A E D C B

17. Topic 2: Chemical Requirements $300 Answer In which of the above tubes are Facultative Anaerobes growing? Correct Answer: B BACK TO GAME A B C D E

18. Topic 2: Chemical Requirements $400 Question All organisms require amino acids to make proteins. How might amino acids be acquired? a. Kreb cycle intermediates b. Environment c. Glycolysis intermediates d. All of the above BACK TO GAME ANSWER

19. Topic 2: Chemical Requirements $400 Answer All organisms require amino acids to make proteins. How might amino acids be acquired? a. Kreb cycle intermediates b. Environment c. Glycolysis intermediates d. All of the above (correct answer) BACK TO GAME

20. Topic 2: Chemical Requirements $500 Question Give an example of a biofilm residing inside the body of an animal. BACK TO GAME ANSWER

21. Topic 2: Chemical Requirements $500 Answer Give an example of a biofilm inside the body of an animal. Examples include: digestive systems, bacterial endocarditis, on teeth BACK TO GAME

22. Topic 3: Growth of Bacterial Cultures $100 Question Generation time can best be defined as a. The length of time it takes for lag phase b. The duration of log phase c. The length of time it takes for a cell to divide d. The amount of time it takes the cell to replicate its chromosome BACK TO GAME ANSWER

23. Topic 3: Growth of Bacterial Cultures $100 Answer Generation time can best be defined as a. The length of time it takes for lag phase b. The duration of log phase c. The length of time it takes for a cell to divide (correct answer) d. The amount of time it takes the cell to replicate its chromosome BACK TO GAME

24. Topic 3: Growth of Bacterial Cultures $200 Question Why can’t obligate anaerobes live in the presence of oxygen (yes, they die, but why?) BACK TO GAME ANSWER

25. Topic 3: Growth of Bacterial Cultures $200 Answer Why can’t obligate anaerobes live in the presence of atmospheric oxygen (yes, they die, but why?) Correct answer: Obligate anaerobes do not produce the enzymes (superoxide dismutase, catalase, peroxidase) necessary to neutralize the toxic forms oxygen takes in the cell BACK TO GAME

26. Topic 3: Growth of Bacterial Cultures $300 Question What is happening in cell A? BACK TO GAME ANSWER A B C

27. Topic 3: Growth of Bacterial Cultures $300 Answer BACK TO GAME What is happening in cell A? Correct answer: DNA Replication A B C

28. Topic 3: Growth of Bacterial Cultures $400 Question Which of the areas in the bacterial growth curve represent the lag phase? BACK TO GAME ANSWER

29. Topic 3: Growth of Bacterial Cultures $400 Answer Which of the areas in the bacterial growth curve represent the lag phase? Correct Answer: 1 BACK TO GAME

30. Topic 3: Growth of Bacterial Cultures $500 Question Why did the population level off at area 3? BACK TO GAME ANSWER

31. Topic 3: Growth of Bacterial Cultures $500 Answer Why did the population level off at area 3? Correct answer: An increase in wastes and decrease in nutrients in an enclosed environment. BACK TO GAME

32. Topic 4: Control of Microbial Growth $100 Question In this graph, an antimicrobial agent was applied. Was this antimicrobial agent Bacteriostatic or Bactericidal BACK TO GAME ANSWER

33. Topic 4: Control of Microbial Growth $100 Answer In this graph, an antimicrobial agent was applied. Was this antimicrobial agent Bacteriostatic (correct answer) BACK TO GAME

34. Topic 4: Control of Microbial Growth $200 Question What will increase the effectiveness of most antiseptics and disinfectants? A) Large number of microbes present B) Presence of endospores C) Presence of organic matter D) Presence of biofilms E) None of the above BACK TO GAME ANSWER

35. Topic 4: Control of Microbial Growth $200 Answer What will increase the effectiveness of most antiseptics and disinfectants? A) Large number of microbes present B) Presence of endospores C) Presence of organic matter D) Presence of biofilms E) None of the above (correct answer) BACK TO GAME

36. Topic 4: Control of Microbial Growth $300 Question What works better- Dry heat or Moist heat? BACK TO GAME ANSWER

37. Topic 4: Control of Microbial Growth $300 Answer What works better- Dry heat or Moist heat? Moist heat (correct answer) BACK TO GAME

38. Topic 4: Control of Microbial Growth $400 Question Radiation primarily affects A) Plasma membranes B) Protein synthesis C) DNA D) Protein structure BACK TO GAME ANSWER

39. Topic 4: Control of Microbial Growth $400 Answer Radiation primarily affects A) Plasma membranes B) Protein synthesis C) DNA (correct answer) D) Protein structure BACK TO GAME

40. Topic 4: Control of Microbial Growth $500 Question Pasteurization is the same as complete sterilization. A) True B) False BACK TO GAME ANSWER

41. Topic 4: Control of Microbial Growth $500 Answer Pasteurization is the same as complete sterilization. A) True B) False (correct answer) BACK TO GAME

42. Topic 5: Misc. $100 Question What are the building blocks of DNA and RNA called? BACK TO GAME ANSWER

43. Topic 5: Misc. $100 Answer What are the building blocks of DNA and RNA called? Nucleotides (correct answer) BACK TO GAME

44. Topic 5: Misc. $200 Question Which is the deoxyribose? BACK TO GAME ANSWER E D C B A

45. Topic 5: Misc. $200 Answer Correct Answer: A BACK TO GAME

46. Topic 5: Misc. $300 Question Nitrogen bases can be ‘complimentary’. What does that mean? BACK TO GAME ANSWER

47. Topic 5: Misc. $300 Answer Nitrogen bases can be ‘complimentary’. What does that mean? Correct answer: A only forms weak hydrogen bonds with T and C only forms weak hydrogen bonds with G. BACK TO GAME

48. Topic 5: Misc. $400 Question A ‘gene’ is a section of DNA that codes for a functional product, such as….. BACK TO GAME ANSWER

49. Topic 5: Misc. $400 Answer A ‘gene’ is a section of DNA that codes for a functional product, such as….. Correct Answer: A protein (primarily) BACK TO GAME

50. Topic 5: Misc. $500 Question In the Keepers of the Biosphere video, relationships between organisms in the rain forest were examined. Fungi in the rain forest had a symbiotic relationship with what organism? BACK TO GAME ANSWER

51. Topic 5: Misc. $500 Answer In the Keepers of the Biosphere video, relationships between organisms in the rain forest were examined. Fungi in the rain forest had a symbiotic relationship with what organism? Correct Answer: Ants BACK TO GAME

52. FINAL ROUND Question An experiment began with 5 cells and ended with 160 cells. How many generations did the cells go through? a. 20 b. 6 c. 4 d. 5 BACK TO GAME ANSWER

53. FINAL ROUND Answer An experiment began with 5 cells and ended with 160 cells. How many generations did the cells go through? a. 20 b. 6 c. 4 d. 5 (correct answer) BACK TO GAME